Validation of RAPD markers associated with frost tolerancein winter faba bean (Vicia faba L.)

A set of 189 single seed lines (validation set, v-set), derived from a natural crossing crossing between 11 founder lines, were used as a different genetic background to validate markers associated with frost tolerance and leaf fatty acid composition content in the winter faba bean. These lines were evaluated in 20 replications with lattice design under three freezing temperatures ( 16 °C, 18 °C, and 19 °C). Two morphological traits, regrowth after frost (REG) and area under symptom progress curve (AUSPC), were scored. In addition, leaf fatty acid composition content was analyzed after hardening conditions (without frost). High significant correlation was found between REG and AUSPC (r =0.58**). AUSPC showed positive significant correlations with C18:0 and C18:2, and negative significant correlations with C18:3. Three RAPD markers were validated in a v-set and were found to be associated with REG and fatty acid contents (C18:0, C18:2, and C18:3). Of these markers, two were found to be associated with more than one trait. The results of the present study indicate that improving the winter faba bean using marker-assisted selection (MAS) is feasible and effective in accelerating breeding programs for producing cultivars with high frost tolerance.

PDF

___

Amar S, Ecke W, Becker HC, Möllers C (2008). QTL for phytosterol and sinapate ester content in Brassica napus L. collocate with the two erucic acid genes. Theor Appl Genet 116: 1051-1061.
Arbaoui M (2007). Detailed genetic analysis of faba bean (Vicia faba L.) winter-hardiness and related traits. PhD, University of Göttingen, Göttingen, Germany.
Arbaoui M, Link W (2007). Effect of hardening on frost tolerance and fatty acid composition of leaves and stems of a set of faba bean (Vicia faba L.) genotypes. Euphytica 162: 211-219.
Arbaoui M, Link W, Satovic Z, Torres AM (2008). Quantitative trait loci of frost tolerance and physiologically related trait in faba bean (Vicia faba L.). Euphytica 164: 93-104.
Basunanda P, Radoev M, Ecke W, Friedt W, Becker HC, Snowdon RJ (2010). Comparative mapping of quantitative trait loci involved in heterosis for seedling and yield traits in oilseed rape (Brassica napus L.). Theor Appl Genet 120: 271-281.
Cheng L, Yang A, Jiang C, Ren M, Zhang Y, Feng Q, Wang S, Guan Y, Luo C (2015). Quantitative trait loci mapping for plant height in tobacco using linkage and association mapping methods. Crop Sci 55: 641.
Chessel D, Dufour A, Thioulouse J (2004). The ade4 package - I: One-table methods. R News 4: 5-10.
Collard BCY, Jahufer MZZ, Brouwer JB, Pang ECK (2005). An introduction to markers, quantitative trait loci (QTL) mapping and marker-assisted selection for crop improvement: the basic concepts. Euphytica 142: 169-196.
Díaz-Ruiz R, Torres AM, Satovic Z, Gutierrez M V, Cubero JI, Román B (2010). Validation of QTLs for Orobanche crenataresistance in faba bean (Vicia faba L.) across environments and generations. Theor Appl Genet 120: 909-919.
Gupta M, Verma B, Kumar N, Chahota RK, Rathour R, Sharma SK, Bhatia S, Sharma TR (2012). Construction of intersubspecific molecular genetic map of lentil based on ISSR, RAPD and SSR markers. J Genet 91: 279-287.
Hassan M (2013). QTL Mapping: Methodology and Applications in Wheat Breeding. Saarbrücken, Germany: LAP LAMBERT Academic Publishing. Herzog H (1988). Winter hardiness in faba beans: varietal differences and interrelations among selection criteria. Plant Breed 101: 269-276.
Kolb FL, Bai G-H, Muehlbauer GJ, Anderson JA, Smith KP, Fedak G (2001). Host plant resistance genes for Fusarium head blight. Crop Sci 41: 611.
Li H, Hearne S, Bänziger M, Li Z, Wang J (2010). Statistical properties of QTL linkage mapping in biparental genetic populations. Heredity 105: 257-267.
Novalina N, Sagala AD (2013). Construction of hevea brasiliensis geneticlinkage map and identification of quantitative trait loci using RAPD markers. Int J Adv Sci Eng Inf Technol 3: 71-75.
Palta JP, Whitaker BD, Weiss LS (1993). Plasma membrane lipids associated with genetic variability in freezing tolerance and cold acclimation of solanum species. Plant Physiol 103: 793-803.
Sallam A, Dhanapal A, Liu S (2015a). Association mapping of winter hardiness and yield traits in winter faba bean (Vicia faba L.). Crop Pasture Sci 67: 55-68.
Sallam A, Martsch R (2015). Association mapping for frost tolerance using multi-parent advanced generation inter-cross (MAGIC) population in faba bean (Vicia faba L.). Genetica 143: 501-514.
Sallam A, Martsch R, Moursi YS (2015b). Genetic variation in morpho-physiological traits associated with frost tolerance in faba bean (Vicia faba L.). Euphytica 205: 395-408.
Singh AKSBD (2015). Marker-Assisted Plant Breeding: Principles and Practices. 1st ed. New Delhi, India: Springer.
Torres AM, Weeden NF, Martín A (1993). Linkage among isozyme, RFLP and RAPD markers in Vicia faba. Theor Appl Genet 85: 937-945.
Williams AF, Tse AG, Gagnon J (1988). Squid glycoproteins with structural similarities to Thy-1 and Ly-6 antigens. Immunogenetics 27: 265-272.
Williams JG, Kubelik AR, Livak KJ, Rafalski JA, Tingey SV (1990). DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res 18: 6531-6535.
Xu L, Wang L, Gong Y, Dai W, Wang Y, Zhu X, Wen T, Liu L (2012). Genetic linkage map construction and QTL mapping of cadmium accumulation in radish (Raphanus sativus L.). Theor Appl Genet 125: 659-670.
Zhao J, Dimov Z, Becker HC, Ecke W, Möllers C (2007). Mapping QTL controlling fatty acid composition in a doubled haploid rapeseed population segregating for oil content. Mol Breed 21: 115-125.