Andre Dias de AZEVEDO NETO, Hewsley Her Baleeiro SILVA, Renata Velasques MENEZES, Petterson Costa Conceiçao SILVA

Use of hydrogen peroxide in acclimation of basil (Ocimum basilicum L.) to salt stress

The aim of this study was to verify the effects of application of H2O2 on salt tolerance and oil production in basil cv. ‘Gennarode Menta’ grown in a hydroponic system. Seedlings were subjected to 3 levels of H2O2 (0, 1, and 1000 μM) in a nutrient solution during2 different exposure periods (24 and 48 h) and 2 levels of NaCl (0 and 80 mM). The plants were harvested at preflowering (at 15 days)and postflowering (at 25 days). At 25 days, the application of 1 μM H2O2/48 h and its reapplication at preflowering increased leafdry mass production compared to the other salt treatments. Relative water content decreased while leaf succulence and sclerophyllyindex increased with salinity. The increase of succulence was more pronounced with the application of H2O2. Salinity increased thechlorophylls content only in plants without the application of H2O2. Salinity increased Cl–, Na+, and organic solutes content, this effectbeing more pronounced in salt treatments that received H2O2. Essential oil content, yield, and productivity were induced by salinity andH2O2. This study shows that H2O2 application in nutrient solution for basil cultivation in hydroponic systems using brackish water is aviable technique for promoting biomass or essential oil yield.

PDF

___

Alves LS, Paz VPS, Silva AJP, Oliveira GXS, Oliveira FER, Amorim EL (2015). Teor, rendimento e composição química do óleo essencial de plantas de manjericão submetidas. Revista Brasileira de Plantas Medicinais 17: 807-813 (article in Portuguese). http://dx.doi.org/10.1590/1983-084X/14_031
Azevedo Neto AD, Prisco JT, Enéas-Filho J, Medeiros JVR, GomesFilho E (2005). Hydrogen peroxide pretreatment induces salt stress acclimation in maize plants. J Plant Physiol 162: 1114- 1122. https://doi.org/10.1016/j.jplph.2005.01.007
Azevedo Neto AD, Prisco JT, Gomes-Filho E (2009). Changes in soluble amino-N, soluble proteins and free amino acids in leaves and roots of salt stressed maize genotypes. J Plant Interact 4: 137-144. https://doi.org/10.1080/17429140902866954
Azevedo Neto AD, Silva EC (2015). Physiology and biochemistry of salt stress tolerance in plants. In: Chakraborty U, Chakraborty B, editors. Abiotic Stresses in Crop Plants. Wallingford, Oxfordshire, UK: CABI, pp. 81-101. https://doi. org/10.1079/9781780643731.0081
Barrs HD, Weatherley PE (1962). A re-examination of the relative turgidity technique for estimating water deficits in leaves. Aust J of Biol Sci 15: 413-428. https://doi.org/10.1071/BI9620413
Bernstein N, Kravchik M, Dudai N (2010). Salinity-induced changes in essential oil, pigments and salts accumulation in sweet basil (Ocimum basilicum) in relation to alterations of morphological development. Ann Appl Biol 156: 167-177. https://doi. org/10.1111/j.1744-7348.2009.00376.x
Bione MAA, Paz VPS, Silva F, Ribas RF, Soares TM (2014). Crescimento e produção de manjericão em sistema hidropônico NFT sob salinidade. Rev Bras Eng Agr Amb 18: 1228-1234 (article in Portuguese). http://dx.doi.org/10.1590/1807-1929/ agriambi.v18n12p1228-1234
Carvalho FEL, Lobo AKM, Bonifácio A, Martins MO, Lima Neto MC, Silveira JAG (2011). Aclimatação ao estresse salino em plantas de arroz induzida pelo pré-tratamento com H2 O2 . Rev
Bras Eng Agr Amb 15: 416-423 (article in Portuguese). http:// dx.doi.org/10.1590/S1415-43662011000400014
Christou A, Manganaris GA, Fotopoulos V (2014). Systemic mitigation of salt stress by hydrogen peroxide and sodium nitroprusside in strawberry plants via transcriptional regulation of enzymatic and non-enzymatic antioxidants. Environ Exp Bot 107: 46-54. https://doi.org/10.1016/j. envexpbot.2014.05.009
Cova AMW, Azevedo Neto AD, Ribas RF, Gheyi HR, Menezes RV (2016). Inorganic solute accumulation in noni (Morinda citrifolia Linn) under salt stress during initial growth. Afr J Agr Res 11: 3347-3354. https://doi.org/10.5897/AJAR2016.11416
Delavari M, Enteshari S, Kalantari KM (2014). Effects of response of Ocimum basilicum to the interactive effect of salicylic acid and salinity stress. Iranian J Plant Physiol 4: 983-990. https://doi. org/10.22034/IJPP.2014.540308
Dias NS, Blanco FF, Souza ER, Ferreira JFS, Sousa Neto ON, Queiroz ISR (2016). Efeitos dos sais na planta e tolerância das culturas à salinidade. In: Gheyi HR, Dias NS, Lacerda CF, Gomes Filho E, editors. Manejo da salinidade na agricultura: Estudos básicos e aplicados. 2nd ed. Fortaleza, CE, BR: INCTSal, pp. 151-162 (in Portuguese).
Ferreira DF (2011). Sisvar: A computer statistical analysis system. Ciênc Agrotec 35: 1039-1042. http://dx.doi.org/10.1590/ S1413-70542011000600001
Furlani PR (1998). Instrução para o cultivo de hortaliça de folha pela técnica de hidroponia– NFT. Campinas: Instituto Agronômico, Boletim Técnico Nº 168 (in Portuguese). Gondim FA, Gomes Filho E, Marques EC, Prisco JT (2011). Efeitos do H2 O2 no crescimento e acúmulo de solutos em plantas de milho sob estresse salino. Rev Ciênc Agron 42: 373-381 (article in Portuguese). http://dx.doi.org/10.1590/S1806- 66902011000200016
Heidari M (2012). Effects of salinity stress on growth, chlorophyll content and osmotic components of two basil (Ocimum basilicum L.) genotypes. Afr J Biotechnol 11: 379-384. https:// doi.org/10.5897/AJB10.489
Lichtenthaler HK, Buschmann C (2001). Chlorophylls and carotenoids: Measurement and characterization by UV-VIS spectroscopy. In: Wrolstad RE, Acree TE, An H, Decker EA, Penner MH, Reid DS, Schwartz SJ, Shoemaker CF, Sporns P, editors. Current Protocols in Food Analytical Chemistry. New York, NY, USA: John Wiley and Sons, pp. 431-438. https://doi. org/10.1002/0471142913.faf0403s01
Lorenzi H, Matos FJA (2008). Plantas medicinais no Brasil: Nativas e exóticas. 2nd ed. Nova Odessa, SP, BRA: Instituto Plantarum. Mengel K (2007). Potassium. In: Barker AV, Pilbeam DJ, editors. Handbook of plant nutrition. 5th ed. New York, NY, USA: CRC Press, pp. 91-120. https://doi.org/10.1007/978-94-010- 1009-2_10
Miranda RS, Mesquita RO, Costa JH, Alvarez-Pizarro JC, Prisco JT, Gomes Filho E (2017). Integrative control between proton pumps and SOS1 antiporters in roots is crucial for maintaining low Na+ accumulation and salt tolerance in ammoniumsupplied Sorghum bicolor. Plant Cell Physiol 58: 522-536. https://doi.org/10.1093/pcp/pcw231
Niu G, Cabrera RI (2010). Growth and physiological responses of landscape plants to saline water irrigation: A Review. Hortscience 45: 1605-1609.
Niu L, Liao W (2016). Hydrogen peroxide signaling in plant development and abiotic responses: Crosstalk with nitric oxide and calcium. Front Plant Sci 7: 1-14. https://doi.org/10.3389/ fpls.2016.00230
Paulus D, Dourado Neto D, Frizzone JA, Soares TM (2010). Produção e indicadores fisiológicos de alface sob hidroponia com água salina. Hortic Bras 28: 29-35 (article in Portuguese). http:// dx.doi.org/10.1590/S0102-05362010000100006
Sacramento BL, Cruz TS, Silva LL, Mota KNAB, Azevedo Neto AD (2014). Pigmentos e teores de solutos orgânicos em plantas de aguapé sob estresse salino. Enciclopédia Biosfera 10 :33-44 (article in Portuguese).
Santos RSS, Dias NS, Duarte SN, Lima CJGS (2012). Uso de águas salobras na produção de rúcula cultivada em substrato de fibra de coco. Rev Caatinga 25: 113-118.
Santos Júnior JA, Gheyi HR, Cavalcante AR, Francilino AH, PerezMarin AM (2016). Crescimento de girassóis ornamentais sob estresse salino em hidroponia de baixo custo. Irriga 21: 591-604 (article in Portuguese). http://dx.doi.org/10.15809/ irriga.2016v21n3p591-604
Selmar D (2008). Potential of salt and drought stress to increase pharmaceutical significant secondary compounds in plants. Landbauforschung - vTI Agric Forest Res 58: 139-144.
Silveira JAG, Araújo SAM, Lima PMS, Viégas RA (2009). Roots and leaves display contrasting osmotic adjustment mechanisms in response to NaCl-salinity in Atriplex nummularia. Environ Exp Bot 66: 1-8. https://doi.org/10.1016/j.envexpbot.2008.12.015
Singh AK, Dubey RS (1995). Changes in chlorophyll a and b contents and activities of photosystems I and II in rice seedlings induced by NaCl. Photosynthetica 31: 489-499.
Taiz L, Zeiger E (2003). Fisiologia vegetal. 5th ed. Porto Alegre, RS, BRA: ARTMED.
Wahid A, Perveen M, Gelani S, Basra SMA (2007). Pretreatment of seed with H2 O2 improves salt tolerance of wheat seedlings by alleviation of oxidative damage and expression of stress proteins. J Plant Physiol 164: 283-294. https://doi.org/10.1016/j. jplph.2006.01.005