Betül DEMİRCİ, Nurhayat TABANCA, A. Ikhlas KHAN, Vijayasankar RAMAN

Studies on the floral anatomy and scent chemistry of titan arum(Amorphophallus titanum, Araceae)

Amorphophallus titanum (Becc.) Becc. ex Arcang., the titan arum, is popularly known for having the world s largest unbranched inflorescence. The enormous flower-like inflorescence is also called corpse flower or carrion flower due to the characteristic putrid odor of the bloom. The present study illustrates detailed anatomy and micromorphology of the inflorescence by light and scanning electron microscopy. Collenchyma was found to be the major tissue providing mechanical support in the spathe. The appendix consisted of a dense parenchymatous cortex enclosing a hollow center traversed by loose networks of aerenchyma. Anthocyanin pigments found in the epidermises and cortical tissues imparted a purple color to various parts of the inflorescence. Two types of raphide crystals, Psychotria type and Lemna type, and cluster crystals were observed. The volatile compounds from different parts of the inflorescence were collected by a modified headspace-solid phase microextraction (HS-SPME) technique and the samples were analyzed by gas chromatography-mass spectrometry (GC-MS). The major volatile compounds found by this method included isovaleric acid (21.6%), butyric acid (17.0%), benzyl alcohol (16.2%), and g-butyrolactone (12.1%) in the appendix; g-butyrolactone (27.0%), tetradecane (13.4%), and 4-hydroxy-4-methyl-2-pentanone (10.5%) in the male flowers; tetradecane (19.9%), 4-hydroxy-4-methyl-2-pentanone (13.0%), and 3-hydroxy-2-butanone (10.6%) in the female flowers; and butyl acetate (44.5%) and 3-hydroxy-2-butanone (12.2%) in the spathe. Use of different types of SPME fibers and multiple analytical methods is necessary in order to obtain a complete picture of the volatile composition of titan arum blooms.

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Barthlott W, Lobin W, editors (1998). Amorphophallus titanum - Tropische und subtropische Pflanzenwelt, Band 99. Stuttgart, Germany: Franz Steiner Verlag (in German).
Barthlott W, Szarzynski J, Vlek P, Lobin W, Korotkova N (2009). A torch in the rain forest: thermogenesis of the titan arum (Amorphophallus titanum). Plant Biol11: 499-505.
Chen G, Gong W, Ge J, Dunn BL, Sun W (2012). Floral scents of typical Buddleja species with different pollination syndromes. Biochem Syst Ecol 44: 173-178.
Endress PK, Baas P, Gregory M (2000). Systematic plant morphology and anatomy: 50 years of progress. Taxon 2000: 401-434.
ESO-2000 (1999). The Complete Database of Essential Oils. Huizen, the Netherlands: Boelens Aroma Chemical Information Service.Filella I, Bosch J, Llusià J, Peñuelas A, Peñuelas J (2011). Chemical cues involved in the attraction of the oligolectic bee Hoplitis aduncato its host plant Echium vulgare. Biochem Syst Ecol 39: 498-508.
Fujioka K, Shirasu M, Manome Y, Ito N, Kakishima S, Minami T, Tominaga T, Shimozono F, Iwamoto T, Ikeda K et al. (2012). Objective display and discrimination of floral odors from Amorphophallus titanum, bloomed on different dates and at different locations, using an electronic nose. Sensors 12: 2152-2161.
Gandawijaja D, Idris S, Nasution R, Nyman L, Arditti J (1983). Amorphophallus titanum Becc.: a historical review and some recent observations. Ann Bot-London 51: 269-278.
Giordano C (1999). Karyological and palynological observations on Amorphophallus titanum (Becc.) Becc. ex Arcangeli (Araceae). Caryologia 52: 65-73.
Giri A, Okamoto A, Okazaki E, Ohshima T (2010). Headspace volatiles along with other instrumental and sensory analyses as indices of maturation of horse mackerel miso. J Food Sci 75: S406-417.
Green PW (2014). Volatile compounds from Liposcelis bostrychophila(Psocoptera: Liposcelididae) and their environment and their effects on settling behaviour. Biochem Syst Ecol 57: 81-89.
Guo N, Gao J, He Y, Zhang Z, Guo Y (2014). Variations in leaf epicuticular n-alkanes in some Broussonetia, Ficus and Humulusspecies. Biochem Syst Ecol 54: 150-156.
Hayat MA (2000). Principles and Techniques of Electron Microscopy: Biological Applications. New York, NY, USA: Cambridge University Press.
Heiduk A, Brake I, Tolasch T, Frank J, Jürgens A, Meve U, Dötterl S (2010). Scent chemistry and pollinator attraction in the deceptive trap flowers of Ceropegia dolichophylla. S Afr J Bot 76: 762-769.
Hejnowicz Z (2005). Unusual metaxylem tracheids in petioles of Amorphophallus (Araceae) giant leaves. Ann Bot-London 96: 407-412.
Hejnowicz Z, Barthlott W (2005). Structural and mechanical peculiarities of the petioles of giant leaves of Amorphophallus (Araceae). Am J Bot 92: 391-403.
Heslop‐Harrison Y (1981). Stigma characteristics and angiosperm taxonomy. Nord J Bot 1: 401-420.
Horner HT, Wagner BL (1995). Calcium oxalate formation in higher plants. In: Khan SR, editor. Calcium Oxalate in Biological Systems. Boca Raton, FL, USA: CRC Press, pp. 53-72.
Johnson S, Jürgens A (2010). Convergent evolution of carrion and faecal scent mimicry in fly-pollinated angiosperm flowers and a stinkhorn fungus. S Afr J Bot 76: 796-807.
Joulain D, König WA (1998). The Atlas of Spectral Data of Sesquiterpene Hydrocarbons. Hamburg, Germany: EB-Verlag.
Katsuno T, Kasuga H, Kusano Y, Yaguchi Y, Tomomura M, Cui J, Yang Z, Baldermann S, Nakamura Y, Ohnishi T et al. (2014). Characterisation of odorant compounds and their biochemical formation in green tea with a low temperature storage process. Food Chem 148: 388-395.
Kite GC, Hetterschieid WL (1997). Inflorescence odours of Amorphophallus and Pseudodracontium (Araceae). Phytochemistry 46: 71-75.
Korotkova N, Barthlott W (2009). On the thermogenesis of the titan arum (Amorphophallus titanum). Pl Signal Behav 4: 1096-1098.
Kundu S, Sinhababu A (2013). Analysis of n-alkanes in the cuticular wax of leaves of Ficus glomerata Roxb. J Appl Nat Sci 5: 226-229.
Lasekan O, Abbas K (2010). Analysis of volatile flavour compounds and acrylamide in roasted Malaysian tropical almond (Terminalia catappa) nuts using supercritical fluid extraction. Food Chem Toxicol 48: 2212-2216.
Levin RA, Raguso RA, McDade LA (2001). Fragrance chemistry and pollinator affinities in Nyctaginaceae. Phytochemistry 58: 429-440.
Liu XS, Liu JB, Yang ZM, Song HL, Liu Y, Zou TT (2014). Aroma-active compounds in jinhua ham produced with different fermentation periods. Molecules 19: 19097-19113.
McLafferty FW, Stauffer DB, Stenhagen E, Heller SR (1989). The Wiley/NBS Registry of Mass Spectral Data. New York, NY, USA: Wiley.
Nadakuduti SS, Pollard M, Kosma DK, Allen C, Jr., Ohlrogge JB, Barry CS (2012). Pleiotropic phenotypes of the sticky peel mutant provide new insight into the role of CUTIN DEFICIENT2 in epidermal cell function in tomato. Plant Physiol 159: 945-960.
Prychid CJ, Jabaily RS, Rudall PJ (2008). Cellular ultrastructure and and crystal development in Amorphophallus (Araceae). Ann Bot 101: 983-995.
Raman V, Horner HT, Khan IA (2014). New and unusual forms of calcium oxalate raphide crystals in the plant kingdom. J Plant Res 127: 721-730.
Selli S, Prost C, Serot T (2009). Odour-active and off-odour components in rainbow trout (Oncorhynchus mykiss) extracts obtained by microwave assisted distillation-solvent extraction. Food Chem 114: 317-322.
Shi X, Zhang X, Song S, Tan C, Jia C, Xia S (2013). Identification of characteristic flavour precursors from enzymatic hydrolysis-mild thermal oxidation tallow by descriptive sensory analysis and gas chromatography-olfactometry and partial least squares regression. J Chromatogr B 913-914: 69-76.
Shirasu M, Fujioka K, Kakishima S, Nagai S, Tomizawa Y, Tsukaya H, Jin M, Manome Y, Touhara K (2010). Chemical identity of a rotting animal-like odor emitted from the inflorescence of the titan arum (Amorphophallus titanum). Biosci Biotech Bioch 74: 2550-2554.
Steenhuisen SL, Raguso R, Jürgens A, Johnson S (2010). Variation in scent emission among floral parts and inflorescence developmental stages in beetle-pollinated Protea species (Proteaceae). S Afr J Bot 76: 779-787.
Tasdemir D, Demirci B, Demirci F, Dönmez AA, Baser K, Rüedia P (2003). Analysis of the volatile components of five Turkish Rhododendron species by headspace solid-phase microextraction and GC-MS (HS-SPME-GC-MS). Z Naturforsch C Bio Sci 58: 797-803.
Ubeda C, San-Juan F, Concejero B, Callejon RM, Troncoso AM, Morales ML, Ferreira V, Hernandez-Orte P (2012). Glycosidically bound aroma compounds and impact odorants of four strawberry varieties. J Agr Food Chem 60: 6095-6102.
van der Pijl L (1937). Biological and physiological observations on the inflorescence of Amorphophallus. Recueil Trav Bot Neerl 34: 157-167.