Mohd GULFISHAN, Ainul Haq KHAN, Tarik Ahmad BHAT

Studies on cytotoxicity induced by DES and SA in Vicia faba var. major

The effect of diethyl sulphate (DES) and sodium azide (SA) was studied on the meiotic behaviour of Vicia faba L. var. major in the M1 generation. Five concentrations of SA (0.01%, 0.02%, 0.03%, 0.04%, and 0.05%) were combined with DES (0.02%) to study the synergistic and antagonistic effects of these 2 mutagens. A dose-dependent increase in chromosomal aberrations was recorded in populations treated with both the mutagens individually and their combination doses. The main chromosomal aberrations were stickiness, precocious separations, univalents, multivalents, laggards, unequal separations of chromosomes, micronuclei, disturbed polarity, multinucleate condition, and cytomixis. The stickiness and stray bivalents at metaphase I/II, bridges with or without fragments at anaphase I/II, disturbed polarity, and cytomixis at telophase I/II were the dominant anomalies. The univalents, rod bivalents, and multivalents showed dose-dependent increases, whereas the ring bivalents and chiasma frequency per cell showed dose-dependent decreases both in single as well as in combined mutagenic treatments as compared to the control.

Anahtar Kelimeler:

Key words: Vicia faba, chromosomal aberrations, DES, SA, cytotoxicity, chiasma frequency

Studies on cytotoxicity induced by DES and SA in Vicia faba var. major

Keywords:

Key words: Vicia faba, chromosomal aberrations, DES, SA, cytotoxicity, chiasma frequency,

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Bhat TA, Khan AH, Parveen S & Ganai FA (2005b). Clastogenic effect of EMS and MMS in Vicia faba L. J Cytol Genet 6: 7-122.
Bhat TA, Khan AH, Parveen S & Ganai FA (2006a). Studies on the effects of EMS on meiosis and pollen fertility in Vicia faba L. Ad Plant Sci 19: 243-247.
Bhat TA, Parveen S & Khan AH (2006b). MMS induced cytomixis in pollen mother cells of broad bean (Vicia faba L.). Turk J Bot 30: 273-279.
Bhat TA, Parveen S & Khan AH (2007). Meiotic studies in two varieties of Viciaf aba L. (Fabaceae) after EMS treatment. Asian J Plant Sci 61: 51-55.
Bhattacharjee SK (1953). Cytogenetics of Lens esculenta Moncsch. Caryologia 5: 159-166.
Dhamyanthi KPM & Reddy VRK (2000). Cytogenetic effect of gamma rays and ethyl methane sulphonate in chilli piper (Capsicum annumm). Cytologia 65: 129-133.
Evans HJ (1962). Chromosome aberrations induced by ionising radiations. Int Rev Cytol 13: 221-232.
Gaulden ME (1987). Hypothesis: Some mutagens directly alter specific chromosomal proteins to produce chromosome stickiness. Mutagenesis 2: 357-365.
Jayabalan N & Rao GR (1987). Gamma radiation induced cytological abnormalities in Lycopersicon esculentum Mill. Var. Pusa Ruby. Cytologia 52: 1-4.
Koul KK (1990). Cytomixis in pollen mother cells of Alopecurus arundinaceus Poir. Cytologia 55: 169-173.
Kumar G & Singh V (2003). Comparative analysis of meiotic abnormalities induced by gamma rays and EMS in barley. J Ind Bot Soc 82: 19-22.
Natrajan AT & Shivshankar G (1965). Studies on modification of mutations responses of barley seeds to ethyl methane sulphonate on the fertility of barley. Red Bot 7: 7-15.
Permjit K & Grover IS (1985). Cytological effects of some organophosphorus pesticides II. Meiotic effects. Cytologia 50: 199-211.
Saylor LG & Smith BN (1996). Meiotic irregularities in species of interspecific hybrids in Pisum. Am J Bot 53: 453-468.
Singh AK (2003). Effect of doses of gamma rays and EMS on germination and plant survival in mungbean Vigna radiata (L.) Wilczek. Int J Mendel 20: 99-102.
Soheir H, Antointee H & Atif H (1989). Cytological effect of herbicide Garlon-4 on root mitosis of Allium cepa. Cytologia 54: 465-472.
Yen C, Yang JL & Sun GL (1993). Intermeiocytic connection and cytomixis in intergeneric hybrids of Roegneria ciliaris (Trin) Nevski with Psathyrstachys huashanica Keng. Cytologia 58: 187- 193.
Zeerak NA (1991). Cytogenetical effect of gamma rays and ethyl methane sulphunate in brinjal (Solanum melongena L.) Cytologia 56: 639-643.