Proximate Composition, Chlorophyll a, and Carotenoid Content in Dunaliella salina (Dunal) Teod (Chlorophycea: Dunaliellaceae) Cultured with Cost-Effective Seaweed Liquid Fertilizer Medium

Growth, proximate composition, chlorophyll a, and carotenoid content were estimated in static cultures of micro alga Dunaliella salina (Dunal) Teod (Chlorophycea: Dunaliellaceae) to compare the quality and quantity of biomass produced with seaweed liquid fertilizer (SLF) against Walne´s medium. Culture of micro algae was performed with controlled temperature of 27 ± 1 °C, salinity of 33, and an irradiance of 120 ± 3 µ mol m-2 s-1, and daily samples were taken to estimate the above-mentioned parameters. Mean cellular density from samples of both medium were similar and growth rates were 0.44 and 0.42 doubling per day, respectively. Maximum values of protein content 5.2 and 5.5 pg per cell were estimated on day 5 in Walne´s medium and SLF, respectively. Carbohydrate and lipid content decreased during the first 4 days corresponding to the culture exponential growth. Higher carbohydrate content was found in both media during the first 2 days and thereafter reduced partially compared to their concentrations. Generally lipid contents in cultures with SLF were significantly higher (P

Proximate Composition, Chlorophyll a, and Carotenoid Content in Dunaliella salina (Dunal) Teod (Chlorophycea: Dunaliellaceae) Cultured with Cost-Effective Seaweed Liquid Fertilizer Medium

Growth, proximate composition, chlorophyll a, and carotenoid content were estimated in static cultures of micro alga Dunaliella salina (Dunal) Teod (Chlorophycea: Dunaliellaceae) to compare the quality and quantity of biomass produced with seaweed liquid fertilizer (SLF) against Walne´s medium. Culture of micro algae was performed with controlled temperature of 27 ± 1 °C, salinity of 33, and an irradiance of 120 ± 3 µ mol m-2 s-1, and daily samples were taken to estimate the above-mentioned parameters. Mean cellular density from samples of both medium were similar and growth rates were 0.44 and 0.42 doubling per day, respectively. Maximum values of protein content 5.2 and 5.5 pg per cell were estimated on day 5 in Walne´s medium and SLF, respectively. Carbohydrate and lipid content decreased during the first 4 days corresponding to the culture exponential growth. Higher carbohydrate content was found in both media during the first 2 days and thereafter reduced partially compared to their concentrations. Generally lipid contents in cultures with SLF were significantly higher (P

___

  • Ben-Amotz A (1999). Production of β-carotene from Dunaliella. In: Cohen Z (ed.) Chemicals from Microalgae, pp. 196 - 204. Padstow, United Kingdom: International Ltd.
  • Bligh EG & Dyer WJ (1959). A rapid method of total lipid extraction and purification. Can J Biochem Physiol 37: 911-917.
  • Brown MR (1991). The amino-acid and sugar composition of 16 species of micro algae used in mariculture. J Exp Mar Biol Ecol 145: 79- 99.
  • Brown MR, Garland CD, Jefrey SW, Jameson ID & Leroi JM (1993). The gross and amino acid compositions of batch and semi-continuous cultures of Isochrysis sp. (clone T-Iso), Pavlova lutheri and Nannochloropsis oculata. J Appl Phycol 5: 285-296.
  • Coutteau P & Sorgeloos P (1992). The use of algae substitutes and the requirement for live algae in the hatchery and nursery rearing of bivalve molluscs: an international survey. J Shellfish Res 11: 467- 476.
  • Fabregas J, Herrero C, Cabezas B & Abalde J (1985). Mass culture and biochemical variability of the marine microalgae Tetraselmis suecica Kylin (Butch) with high nutrient concentrations. Aquaculture 49: 231-244.
  • Fernandez-Reiriz MJ, Perez-Camacho A, Ferreiro MJ, Blanco J, Planas M, Campos MJ & Labarta U (1989). Biomass production and variation in the biochemical profile (total protein, carbohydrates, RNA, lipids and fatty acids) of seven species of marine micro algae. Aquaculture 83: 17-37.
  • Garcia-Gonzalez M, Manzano JC, Moreno J & Guerrero MG (2000). Biotecnologıa del cultivo de Dunaliella salina en el litoral andaluz. pp. 16/00: 163. Spain: Pescay Acuicultura, Consejeria de Agriculturay Pesca.
  • Gireesh R, Smitha KS, Bindhu KB & Gopinathan CP (2001). Dunaliella salina – a nonconventional live feed. In: Menon NG & Pillai NGK (eds) Perspectives in Mariculture, pp. 235-240. India: Marine Biological Association of India.
  • Herrero C, Cid A, Fabregas J & Abalde J (1991). Yields in biomass and chemical constituents of four commercially important marine micro algae with different culture media. Aquacult Eng 10: 99-110.
  • Jin E, Feth B & Melis A (2003). A mutant of the green alga Dunaliella salina constitutively accumulates zeaxanthin under all growth conditions. Biotechnology and Bioengineering 81: 115-124.
  • Kaladharan P, Gireesh R & Smitha KS (2002). Cost effective medium for the laboratory culture of live feed micro algae. Seaweed Res Utiliz 24: 35-40.
  • Kaladharan P, Gopinathan CP & Sridhar N (1999). Basic biochemical constituents in the laboratory cultures of six species of micro algae. In: Mohan JM, Menon NR & Nair NV (eds) The Fourth Indian Fisheries Forum Proceedings, pp. 311-312. Mangalore, India: Asian Fisheries Society.
  • Kochert G (1978). Carbohydrate determination by phenol-sulfuric acid method. In: Hellebust JA & Craige JS (eds) Handbook of Physiological and Biochemical Methods, pp. 95-97. London: Cambridge University Press.
  • Lopez-Elias J & Voltolina D (1993). Cultivos semicontinuos de cuatro especies de microalgas con un medio no convencional. Ciencias Marinas 19: 169-180.
  • Lopez-Ruız JL, Garcia-Garcia R & Ferreiro-Almeda MS (1995). Marine micro algae culture: Chaetoceros gracilis with zeolitic product zeestec-56 and a commercial fertilizer as a nutrient. Aquacult Eng 14: 367-372.
  • Lowery OH, Rosebrough NH & Fair AL (1969). Protein measurement with the Folin phenol reagent. J Biol Chem 193: 265-275.
  • Myklestad S (1974). Production of carbohydrates by marine planktonic diatoms. I. comparison of nine different species in culture. J Exp Mar Biol Ecol 15: 261-274.
  • Nieves M, Voltolina D, Sapien MT, Gerhardus H, Robles AI & Villa MA (1996). Coltivazione di microalghe con fertilizzanti agricoli. Revista Italiana di Acquacolture 31: 81-84.
  • Pande SV, Parvin R & Venkitasubramanian (1963). Microdetermination of lipids and serum total fatty acids. Anal Biochem 6: 415-425.
  • Parson TR, Maita Y & Lalli CM (1985). A Manual of Chemical and Biological Methods for Seawater Analysis. New York: First Pergamon Press.
  • Renaud SM, Thinh Luong-Van & Perry DL (1999). The gross chemical composition and fatty acid composition of 18 species of tropical Australian micro algae for possible use in mariculture. Aquaculture170: 147-159.
  • Stella R & Ami BA (1986). Effect of NaCl concentration on cadmium uptake by the halophilic alga Dunaliella salina. Mar Ecol Prog Ser 30: 215-219.
  • Valenzuela-Espinoza E, Millan-Nunez R & Nunez-Cebrero F (1999). Biomass production and uptake by Isochrysis aff. galbana (clone T- Iso) cultured with a low cost alternative to the f/2 medium. Aquacult Eng 20: 135-147.
  • Walne PR (1974). Culture of bivalve molluscs. 50 years experience at Conway. United Kingdom: Fishing News Books Ltd.
  • Whyte JNC (1987). Biochemical composition and energy content of six species of phytoplankton used in mariculture of bivalves. Aquaculture 60: 231-241.
  • Whyte JNC, Bourne N & Hodgson CA (1989). Influence of algal diets on biochemical compositions and energy reserves in Platinopecten yessoensis (Jay) larvae. Aquaculture 78: 333-347.
  • Wikfors G (1986). Altering growth and gross chemical composition of two micro algal molluscan food species by varying nitrate and phosphate. Aquaculture 59: 1-14.
  • Zhu CJ, Lee YK & Chao TM (1997). Effects of temperature and growth phase on lipid and biochemical composition ofIsochrysis galbana TK1. J Appl Phycol 9: 451-457.
Turkish Journal of Botany-Cover
  • ISSN: 1300-008X
  • Yayın Aralığı: Yılda 6 Sayı
  • Yayıncı: TÜBİTAK