Ployphenol oxidase activity during rooting in cuttings of grape (Vitis vinifera L.) varieties
Farklı asma (Vitis vinifera 1. cvs. Muscat, Cardinal ve Perlelte) çeliklerinde koklenme sırasındaki polifenol oksidaz (PPO) aktivitesi incelenmiş ve enzim aktivitesi ile koklenme yeteneği arasındaki ilişki karşılaştırılmıştır. Bu çalışmada, Muscat ve Perlelte çeliklerinde koklenme oluşurken, Cardinal çeliklerinde oluşmadı. Muscat ve Perlelte çeliklerinin PPO aktivitesinde başlangıçtan itibaren artış görülmüş ve köklenmeyle birlikte azalma görülmüştür. Cardinal çeliklerinde ise PPO aktivitesi sürekli artmaya devam etmiştir. Sonuç olarak, çeliklerin PPO aktivitesi ile koklenme yeteneği arasında belirgin bir ilişki bulunamamıştır.
Farklı asma (Vitis vinifera L.) variyetlerinin çeliklerinde köklenme sırasındaki polifenol oksidaz aktivitesi
Polyphenol oxidase (PPO) activity was investigated during rooting in cuttings from three different grape cultivars (Vitis vinifera I. cvs. Muscat, Cardinal and Perlelte), and the enzyme activity and rooting ability were compared. Rooting was observed on the Muscat and Perlelte cuttings, but not on the Cardinal cuttings. PPO activity started to increase in the early stage of the experiment, and decreased after root emergence in the Muscat and Perlelte cuttings. However, enzyme activity started to increase in the early stages and continued throughout the experiment in the Cardinal cuttings. No apparent correlation was found between PPO activity and rooting ability in the cutting.
___
- 1. Cash JN, Sistrunk WA & Stutte CA (1976). Characterization of catecholase and cresolase activities in Monastrell grape polyphenol oxidase. J Food Sci 41:1398-1402.
- 2. Dalet F & Cornu D (1988). Lignification levels and peroxidase activity during in vitro rooting of Prunus avium. Can J Bot 67: 2192-2186.
- 3. Gonzales R, Sanchez R & Rodriguez R (1991). Ethylene in relation to protein, peroxidase and polyphenol oxidase activities during rooting in hazelnut cotyledons. Phys Platarum 83: 611-620.
- 4. Hahlbrock K & Grisebach H (1979). Enzymatic controls in the biosynthesis of lignin and flavonoids. Annu Rev Plant Phys 30: 105-136.
- 5. Huystee RB & Cairns WL (1982). Progreno and prospects in the use of peroxidase to study cell development. Phytochem 21: 1843-1847.
- 6. Vaughn KC & Duke SO (1984). Tentoxin stops the processing of polyphenol into an active enzyme. Plant Physiol 60: 106-112.
- 7. Whitaker JR (1972). Principles of Enzymology for the Food Sciences. New York: Marcel Dekker Inc.
- 8. Wong TC, Luh BS & Whitaker JR (1971). Isolation and characterization of polyphenol oxidase isoenzyme of Clingstone peach. Plant Physiol 48: 19-23.