Marko SABOVLJEVIC, Aneta BIJELOVIC, Ivana DRAGICEVIC

In vitro cultre of mosses: Aloina alodes (K.F.Schultz) Kindb.,Brachythecium velutinum (Hedw.) B.S.& G., Ceraton purpureus (Hedw.) Brid.,Eurhynchium praelongum (Hedw.) B.S.& G. and Grimmia pulvinata (Hedw.) Sm.

Hormonsuz veya farklı konsantrasyonlarda oksin ve sitokinin içeren besi ortamlarında (MS) yaşamları tehlikede olmayan çok sayıdaki yosun türü için in vitro kültürleri oluşturulmuştur. Eurhynchium praelongum (Hedw.) B.S. & G.'nin in vitro kültürüne gametofitlerin uç sürgünlerinden, Aloina aloides (K.F.Schultz) Kindb., Brachythecium velutinum (Hedw.) B.S. & G., Ceratodon purpureus (Hedw.) Brid. ve Grimmia pu/vınata(Hedw.) Sm.'nin in vitro kültürlerine ise olgunlaşmamış saprofitlerin sporlarından alınarak başlanmıştır. Eurhynchium praelongum'nln sekunder protonema kültürü doğal olarak başarılı bir şekilde oluşmaktadır. Aloina aloides, Brachythecium velutinum ve Ceratodon purpureus'nm protonema kültürleri tomurcuk formasyonunun gerçekleştiği kaulonema kültürüne dönüşmüş, Grimmia pulvinata ise protonema evresinde kalmıştır.

Aloina aloides (K.F.Schultz) Kindb.,Brachythecium velutinum (Hedw.) B.S& G. Ceratodon purpureus (Hedw.) Brid., Eurhynchium praelongum (Hedw.) B.S.& G. ve Grimmia pulvinata (Hedw.) Sm. yosunlarının in vitro kültürü

In vitro cultures of five moss species were established on hormone-free MS medium, or on MS medium supplemented with different concentrations of 2,4-dichlorophenoxyacetic acid and kinetin. In vitro culture of Eurhynchium praelongum (Hedw.) B., S. & G., has been initiated from the apical shoots of the gametophytes, and the cultures of Aloina aloides (K.F.Schultz) Kindb. Brachythecium velutinum (Hedw.) B., S. &G., Ceratodon purpureus (Hedw.) Brid. and Grimmia pulvinata (Hedw.) Sm. were initiated from the spores of immature sporophytes. In secondary protonema culture of Eurhynchium praelongum, spontaneous regeneration occurred successfully. Protonema cultures of Aloina aloides, Brachythecium velutinum and Ceratodon purpureus reversed to caulonema culture where bud formation occurred. Grimmia pulvinata cultures remained at the protonema stage.

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