Effects of arbuscular mycorrhizal inoculation on biochemical parameters in Capsicum annuum grown under long term salt stress

Salt stress is an important environmental stress. Plants cope with salt stress with different strategies. In this study the effects of 2 different arbuscular mycorrhiza species (Glomus mosseae and G. intraradices) on some biochemical parameters in pepper plants (Capsicum annuum L. cv. Cumaovası) exposed to long term salt stress were studied. It was found that mycorrhizal inoculation increased RWC, P, total chlorophyll, and carotenoid content of pepper plants during salt application. The enzyme activities changed depending on the enzyme and salt stress application. The lowest MDA content was found in the plants inoculated with G. intraradices; however, there was no significant difference between the NaCl applications. It was found that plants inoculated with G. intraradices had less lipid peroxidation, and therefore it can be said that these plants have an advantage under salt stress.

Effects of arbuscular mycorrhizal inoculation on biochemical parameters in Capsicum annuum grown under long term salt stress

Salt stress is an important environmental stress. Plants cope with salt stress with different strategies. In this study the effects of 2 different arbuscular mycorrhiza species (Glomus mosseae and G. intraradices) on some biochemical parameters in pepper plants (Capsicum annuum L. cv. Cumaovası) exposed to long term salt stress were studied. It was found that mycorrhizal inoculation increased RWC, P, total chlorophyll, and carotenoid content of pepper plants during salt application. The enzyme activities changed depending on the enzyme and salt stress application. The lowest MDA content was found in the plants inoculated with G. intraradices; however, there was no significant difference between the NaCl applications. It was found that plants inoculated with G. intraradices had less lipid peroxidation, and therefore it can be said that these plants have an advantage under salt stress.

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  • Aebi HE, Bergmayer J & Grabl M (1983). Catalase In: Methods of Enzymatic Analysis, pp. 273-286. Weinheim: Verlag Chemie.
  • Al-Khaliel AS (2010). Eff ect of salinity stress on mycorrhizal association and growth response of peanut infected by Glomus mosseae. Plant Soil Environ 56: 318-324.
  • Aliasgharzadeh N, Rastin SN, Towfi ghi H & Alizadeh A (2001). Occurrence of arbuscular mycorrhizal fungi in saline soils of the Tabriz Plain of Iran in relation to some physical and chemical properties of soil. Mycorrhiza 11: 119-122.
  • Beltrano J & Ronco MG (2008). Improved tolerance of wheat plants (Triticum aestivum L.) to drought stress and rewatering by the arbuscular mycorrhizal fungus Glomus claroideum: Eff ect on growth and cell membrane stability. Brazilian Journal of Plant Physiology 20: 29-37.
  • Beyer WF & Fridowich I (1987). Assaying for superoxide dismutase activity: Some large consequences of minor changes in conditions. Anal Biochem 161: 559-566.
  • Blilou P, Bueno JA & Ocampo Garcia-Garrido J (2000). Induction of catalase and ascorbate peroxidase activities in tobacco roots inoculated with the arbuscular mycorrhizal Glomus mosseae. Mycol Res 104: 722-725.
  • Bonnet M, Camares O & Veisserie P (2000). Eff ects of zinc and infl uence of Acremonium lolii on growth parameters, chlorophyll a fl urescence and antioxidant enzyme activities of ryegrass. J Exp Bot 51: 945-953.
  • Caravaca F, Alguacil MM, Hernández JA & Roldán A (2005). Involvement of antioxidant enzyme and nitrate reductase activities during water stress and recovery of mycorrhizal Myrtus communis and Phillyrea angustifolia plants. Plant Sci 169: 191-197.
  • Carlberg I & Mannervik B (1985). Glutation Reductase. Method Enzymol 113: 484-490.
  • Cekic FO & Unyayar S (2006). Interactive eff ects of NaCl and CdCl on the antioxidant enzyme activities and some 2 biochemical compounds in two tomato genotypes. Fresen Environ Bull 15: 633-639.
  • Cho K, Toler H, Lee J, Ownley B, Stutz JC, Moore JL & Auge RM (2006). Mycorrhizal symbiosis and response of sorghum plants to combined drought and salinity stresses. J Plant Physiol 163: 517-528.
  • Daei G, Ardekani MR, Rejali F, Teimuri S & Miransari M (2009). Alleviation of salinity stress on wheat yield, yield components, and nutrient uptake using arbuscular mycorrhizal fungi under fi eld conditions. J Plant Physiol 166: 617-625.
  • Davies Jr FT, Potter JR & Linderman RG (1992). Mycorrhiza and repeated drought exposure aff ect drought resistance and extraradical hyphae development of pepper plants independent of plant size and nutrient content. J Plant Physiol 139: 289-294.
  • Demir S (2004). Infl uence of arbuscular mycorrhiza on some physiological growth parameters of pepper. Turk J Biol 28: 85-90.
  • Giovannetti M & Mosse B (1980). An evaluation of techniques for measuring vesicular-arbuscular mycorrhiza in roots. New Phytol 84: 489-500.
  • Hartree EF (1972). Determination of protein: A Modifi cation of Lowry Method that Gives a Linear Photometric Response. Anal Biochem 48: 422-427.
  • Juniper S & Abbott L (1993). Vesicular arbuscular mycorrhizas and soil salinity. Mycorrhiza 4: 45-57.
  • Kaya C, Ashraf M, Sonmez O, Aydemir S, Tuna AL & Cullu MA (2009). Th e infl uence of arbuscular mycorrhizal colonisation on key growth parameters and fruit yield of pepper plants grown at high salinity. Scientia Horticulturae 121: 1-6.
  • Koca H, Ozdemir F & Turkan I (2006). Eff ect of salt stress on lipid peroxidation and superoxide dismutase and peroxidase activities of Lycopersicon esculentum and L. pennellii. Biol Plantarum 50: 745-748.
  • Koske RE & Gemma JN (1989). A modifi ed procedure for staining roots to detect vam. MycolRes 92: 486-505.
  • Lambais MR, Rios-Ruiz WF & Andrade RM (2003). Antioxidant responses in bean (Phaseolus vulgaris) roots colonized by arbuscular mycorrhizal fungi. New Phytol 160: 421-428.
  • Malan C, Greyling MM & Gressel J (1990). Correlation between CuZn superoxide dismutase and glutathione reductase and environmental and xenobiotic stress tolerance in maize inbreds. Plant Sci 69: 157-166.
  • Martin CA & Stutz JC (2004). Interactive eff ects of temperature and arbuscular mycorrhizal fungi on growth, P uptake and root respiration of Capsicum annuum L. Mycorrhiza 14: 241-244.
  • Marulanda A, Porcel R, Barea JM & Azcón R (2007). Drought tolerance and antioxidant activities in lavender plants colonized by native drought-tolerant or drought-sensitive Glomus species. Microb Ecol 54: 543-552.
  • Miransari M, Bahrami HA, Rejali F & Malakouti MJ (2009). Eff ects of soil compaction and arbuscular mycorrhiza on corn (Zea mays L.) nutrient uptake. Soil Till Res 103: 282- 290.
  • Moore TC (1974). Research Experiences in Plant Physiology. New York: Springer Verlag.
  • Munir N & Aft ab F (2009). Th e role of polyethylene glycol (PEG) pretreatment in improving sugarcane’s salt (NaCl) tolerance. Turk J Bot 33: 407-415.
  • Murphy Y & Riley JP (1962). A modifi ed single solution method for determination of phosphate in natural waters. Anal Chim Acta 27: 31-36.
  • Ohkawa H, Ohishi N & Yagi Y (1979). Assay of lipid peroxides in animal tissue by thiobarbituric acid reaction. Anal Biochem 95: 351-358.
  • Ortas I & Akpınar C (2006).Response of kidney bean to arbuscular mycorrhizal inoculation and mycorrhizal dependency in P and Zn defi cient soils. Acta Agr Scand B-S P 56: 101-109.
  • Paranychianakis NV & Chartzoulakis KS (2005). Irrigation of Mediterranean crops with saline water: from physiology to management practices. Agriculture, Ecosystems and Environment 106: 171-187.
  • Parida AK & Das AB (2005). Salt tolerance and salinity eff ects on plants: a review. Ecotox Environ Safe 60: 324-349.
  • Porcel R, Barea JM & Ruiz JM (2003). Antioxidant activities in mycorrhizal soybean plants under drought stress and their possible relationship to the process of nodule senescence. New Phytol 157: 135-143.
  • Porcel R & Ruiz-Lozano M (2004). Arbuscular mycorrhizal infl uence on leaf water potential, solute accumulation, and oxidative stress in soybean plants subjected to drought stress. J Exp Bot 55: 1743-1750.
  • Porra RJ, Th ompson RA & Kriedemann PE (1989). Determination of accurate extinction coeffi cients and simultaneous equations for assaying chlorophylls a and b extracted with four diff erent solvent verifi cation of the concentration of chlorophyll standards by atomic absorption spectroscopy. Biochimica et Biophysica Acta 975: 384-394.
  • Rahmaty R & Khara J (2011). Eff ects of vesicular arbuscular mycorrhiza Glomus intraradices on photosynthetic pigments, antioxidant enzymes, lipid peroxidation, and chromium accumulation in maize plants treated with chromium. Turk J Biol 35: 51-58.
  • Rodriguez RJ, Redman RS & Henson JM (2004). Th e role of fungal symbioses in the adaptation of plants to high stress environments. Mitigation and Adaptation Strategies for Global Change 9: 261-272.
  • Roldán A, Díaz-Vivancos P, Hernández JA, Carrasco L & Caravaca F (2008). Superoxide dismutase and total peroxidase activities in relation to drought recovery performance of mycorrhizal shrub seedlings grown in an amended semiarid soil. J Plant Physiol 165: 715-722.
  • Ruiz-Lozano JM, Azcón R & Gómez M (1996). Alleviation of salt stress by arbuscular-mycorrhizal Glomus species in Lactuca sativa plants. Physiol Plantarum 98: 767-772.
  • Ryan MH & Graham JH (2002). Is there a role for arbuscular mycorrhizal fungi in production agriculture? Plant Soil 244: 263-271.
  • Sairam RK & Srivastava GC (2002). Changes in antioxidant activity in sub-cellular fractions of tolerant and susceptible wheat genotypes in response to long term salt stress. Plant Sci 162: 897-904.
  • Sensoy S, Demir S, Turkmen O, Erdinc C & Savur OB (2007). Responses of some diff erent pepper (Capsicum annuum L.) genotypes to inoculation with two diff erent arbuscular mycorrhizal fungi. Scientia Horticulturae 113: 92-95.
  • Smith SE & Read DJ (2008). Mycorrhizal Symbiosis. San Diego, CA: Academic Press.
  • Wheeler TR, Crawford PQ, Ellis RH, Porter JR & Vara Prasad PV (2000). Temperature variability and the yield of annual crops. Agr Ecosyst Environ 82: 159-167.
  • Wu QS, Zou YN & Xue Xia R (2006). Eff ects of  water stress and  arbuscular mycorrhizal fungi on  reactive oxygen metabolism and  antioxidant production by  citrus (Citrus tangerine) roots. Eur J Soil Biol 42: 166-172.
  • Wu QS & Zou YN (2010). Benefi cial roles of arbuscular mycorrhizas in citrus seedlings at temperature stress. Scientia Horticulturae 125: 289-293.
  • Yang X, Wang X & Wei M (2010). Response of photosynthesis in the leaves of cucumber seedlings to light intensity and CO 2 concentration under nitrate stress. Turk J Bot 34: 303-310.
  • ZhongQun H, Chao Xing H, Zhi Bin Z, Zhi Rong Z & Huai Song W (2007). Changes of antioxidative enzymes and cell membrane osmosis in tomato colonized by arbuscular mycorrhizae under NaCl stress. Colloids and Surfaces B: Biointerfaces 59: 128-133.