Air pollution effects on flavonoids in pollen grains of some ornamental plants

Phenolic compounds function as stress indicators because they accumulate to high levels in many plant tissues in response to a wide range of biotic and abiotic signals. These compounds are involved in pollen development, pollination, pollen germination, and pollen tube growth. The mature pollen grains of Spartium junceum L., Lagerstroemia indica L., Thuja orientalis L., and Petunia hybrida L. collected from control (less polluted) and polluted areas [mainly SO2, NO2, CO, hydrocarbons (HC), and airborne particulate material (APM)]. The ethanolic aquatic extracts of pollen grains were prepared. The extracts were analysed by high pressure liquid chromatography (HPLC). HPLC analysis demonstrated that air pollution induces flavonoids accumulation to significantly higher levels in the polluted pollen of Spartium junceum, Lagerstroemia indica, and Thuja orientalis than in the controls. In Petunia hybrida the flavonoids level increased more slightly in the pollen grains exposed to air pollutants compared to controls.

Air pollution effects on flavonoids in pollen grains of some ornamental plants

Phenolic compounds function as stress indicators because they accumulate to high levels in many plant tissues in response to a wide range of biotic and abiotic signals. These compounds are involved in pollen development, pollination, pollen germination, and pollen tube growth. The mature pollen grains of Spartium junceum L., Lagerstroemia indica L., Thuja orientalis L., and Petunia hybrida L. collected from control (less polluted) and polluted areas [mainly SO2, NO2, CO, hydrocarbons (HC), and airborne particulate material (APM)]. The ethanolic aquatic extracts of pollen grains were prepared. The extracts were analysed by high pressure liquid chromatography (HPLC). HPLC analysis demonstrated that air pollution induces flavonoids accumulation to significantly higher levels in the polluted pollen of Spartium junceum, Lagerstroemia indica, and Thuja orientalis than in the controls. In Petunia hybrida the flavonoids level increased more slightly in the pollen grains exposed to air pollutants compared to controls.

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  • Alscher RG, Donahue JL & Cramer CL (1997). Reactive oxygen spe- cies and antioxidants: relationships in green cells. Physiol Plant 100: 224-233.
  • Bors W, Michel C & Saran M (1994). Flavonoid antioxidants: rate constants for reactions with oxygen radicals. Methods Enzymol 234: 420-429.
  • Bortnick SM & Stetzer SL (2002). Sampling frequency guidance for ambient air toxics monitoring. J Air Waste Manage Assoc 52: 867-875.
  • Burbulis IE, Iacobucci M & Shirley BW (1996). Null mutation in the fi rst enzyme of fl avonoid biosynthesis does not aff ect male fertility in Arabidopsis. Plant Cell 8: 1013-1025.
  • Campos M, Markham KR, Mitchell KA & Proenca da Cunha A (1997). An approach to the characterization of bee pollens via their fl avonoid/phenolic profi les. Phytochem Anal 8: 181-185.
  • Chaves N, Escudero JC & Gutiérrez-Merino C (1993). Seasonal variation of exudate of Cistus ladanifer. J Chem Ecol 19: 2577- 2591.
  • Cooper-Driver GA & Bhattacharya M (1998). Role of phenolics in plant evolution. Phytochem 49: 1165-1174.
  • Dixon RA. & Paiva NL (1995). Stress-Induced Phenylpropanoid Me- tabolism. Plant Cell 7: 1085-1097.
  • Emberlin J (2000). Th e problems of pollen. Current Medical Literature (CML): Allergy 8: 25-28.
  • Giertych MJ & Karolewski P (1993). Changes in phenolic compounds content in needles of Scots pine (Pinus sylvestris L.) seedlings following short term exposition to sulphur dioxide. Arbor Kórnickie 38: 43-52.
  • Giertych MJ, Karolewski P & De Temmerman LO (1999). Foliage age and pollution alter content of phenolic compounds and chemical elements in Pinus nigra needles. Water Air Soil Pollut 110: 363-377.
  • Karolewski P & Giertych MJ (1995). Changes in the level of phenols during needle development in Scots-pine populations in a control and polluted environment. Eur J Plant Pathol 25: 297- 306.
  • Loponen J, Lempa K, Ossipov V, Kozloov MV, Girs A, Hangasmaa K, Haukioja E & Pihlaja K (2001). Patterns in content of phenolic compounds in leaves of mountain birches along a strong pollution gradient. Chemosphere 45: 291-301.
  • Murphy A, Peer WA & Taiz L (2000). Regulation of auxin transport by aminopeptidases and endogenous fl avonoids. Planta 211: 315-324.
  • Nandi PK, Agrawal M, Agrawal SB & Rao DN (1990). Physiological responses of Vicia faba L. to sulfur dioxide. Ecotoxicol Environ Safe 19: 64-71.
  • Pisani JM & Distel RA (1998). Inter and intraspecifi c variations in production of spines and phenols in Prosopis caldenia and Prosopis fl exuosa. J Chem Ecol 24: 23-36.
  • Rezanejad F (2007). Th e eff ect of air pollution on microsporogenesis, pollen development and soluble pollen proteins in Spartium junceum L. (Fabaceae). Turk J Bot 31: 183-191.
  • Rezanejad F, Majd A, Shariatzadeh SMA, Moein M, Aminzadeh M & Mirzaeian M (2003). Eff ect of air pollution on pollen soluble proteins, structure and cellular material release in Lagerstroemia indica. Acta Biol Cracov Ser Bot 45: 129-132.
  • Rhodes MJC (1994). Physical role for secondary metabolites in plants: Some progress, many outstanding problems. Plant Mol Biol 24: 1-20.
  • Robles C, Greff S, Pasqualini V, Garzino S, Bousquet-Mélou A, Fernandez C, Korboulewsky N & Bonin G (2003). Phenols and fl avonoids in Aleppo pine needles as bioindicators of air pollution. J Environ Qual 32: 2265-2271.
  • Takahama U (1989). A role of hydrogen peroxide in the metabolism of phenolics in mesophyll cells of Vicia faba L. Plant Cell Physi- ol 30: 295-301.
  • Van Tunen AJ, Mur LA, Brouns GS, Rienstra J, Koes RE & Mol JNM (1990). Pollen and anther specifi c chi promoters from Petunia: tandem promoter regulation of the chiA gene. Plant cell 2: 393- 401.
  • Vogt T, Pollak P, Tarlyn N & Taylor LP (1994). Pollination- or wound-induced Kaempferol accumulation in Petunia stigmas enhances seed production. Plant Cell 6: 11-23.
  • Wolters JHB & Martens MJM (1987). Eff ects of air pollutants on pollen. Bot Rev 53: 372-414.
  • Woo HH, Jeong BR & Hawes MC (2005). Flavonoids: From cell cycle to biotechnology. Biotechnol Lett 27: 365-374.
  • Yamasaki H (1997). A function of colour. Trends Plant Sci 2: 7-8.
  • Ylstra B, Busscher J, Franken J, Hollman PCH, Mol JNM & van Tunen AJ (1994). Flavonols and fertilization in Petunia hybrida: localization and mode of action during pollen tube growth. Plant J 6: 201-212.
  • Zobel A & Nighswander JE (1991). Accumulation of phenolic compounds in the necrotic areas of Austrian and red pine needles aft er spraying with sulphuric acid: A possible bioindicator of air pollution. New Phytol 117: 565-574.