Mehtap Şahin ÇEVİK, Gloria A. MOORE

Construction of a Genetic Linkage Map of Citrus with Random Amplified Polymorphic DNA (RAPD) Markers Using a Progeny Population from a Complex Intergeneric Cross

This study included 30 plants that were randomly selected from a larger progeny population obtained from a complex intergeneric cross of {C. grandis (L.) Osb. x [C. paradisi Macf. x Poncirus trifoliata (L.) Raf.]} x {[(C. paradisi Macf. x P. trifoliata (L.) Raf.) x C. reticulata Blanco] x [(C. paradisi Macf. x Poncirus trifoliata (L.) Raf.) x C. sinensis (L.) Osb.]}. Genomic DNA was extracted from leaf samples of these plants and analysed for polymorphisms by polymerase chain reaction (PCR), using 10-mer random primers. A total of 111 random amplified polymorphic DNA (RAPD) markers were identified using 38 random primers. A genetic linkage map of the progeny population was constructed with these RAPD markers. The map contains 63 markers distributed into 9 linkage groups, which possibly correspond to the 9 haploid chromosomes of Citrus. The total maximum length of the linkage map generated in this study was 314.8 cM, with an average map distance of 5.07 cM between markers.

Anahtar Kelimeler:

Citrus, DNA markers, RAPD markers, genetic linkage mapping

Construction of a Genetic Linkage Map of Citrus with Random Amplified Polymorphic DNA (RAPD) Markers Using a Progeny Population from a Complex Intergeneric Cross

Keywords:

Citrus, DNA markers, RAPD markers, genetic linkage mapping,

PDF

___

Asins MJ, Bernet GP, Ruiz C, Cambra M, Guerri J & Carbonell EA (2004). QTL analysis of citrus tristeza virus-citradia interaction. Theor Appl Genet 108: 603-611.
Bernet GP, Margaix C, Jacas J, Carbonell EA & Asins MJ (2005). Genetic analysis of citrus leafminer susceptibility. Theor Appl Genet 110: 1393-1400.
Cai Q, Guy CL & Moore GA (1994). Extension of the linkage map in Citrus using random amplified polymorphic DNA (RAPD) markers and RFLP mapping of cold- Genetics 89: 606-614. acclimation responsive loci.
Clark MS & Wall WJ (1996). Chromosomes: The Complex Code. New York: Chapman and Hall.
Cristofani M, Machado MA & Grattapaglia D (1999). Genetic linkage maps of Citrus sunki Hort. ex Tan. and Poncirus trifoliate (L.) Raf. and mapping of citrus tristeza virus resistance gene. Euphytica 109: 25-32.
Deng ZN, Gentile A, Nicolosi E, Domina F, Vardi A & Tribulato E (1995). Identification of in vivo and in vitro lemon mutants by RAPD markers. J Hort Sci 70: 117-125.
Durham RE, Liou PC, Gmitter FG Jr & Moore GA (1992). Linkage of restriction fragment length polymorphisms and isozymes in citrus. Theor Appl Genet 84: 39-48.
Echt CS, Knapp SJ & Liu BH (1992). Genome mapping with non-inbred crosses using Gmendel 2.0. Maize Genet Coop Nwsl 66: 27-29.
Edwards KJ & Thompson C (1991). A simple and rapid method for the preparation of plant genomic DNA for PCR analysis. Nucleic Acids Res 19: 1349-1355.
Garcia R, Asíns MJ, Forner J & Carbonell EA (1999). Genetic analysis of apomixis in Citrus and Poncirus by molecular markers. Theor Appl Genet 99: 511-518.
Jarrell DC, Roose ML, Traugh SN & Kupper RS (1992). A genetic map of citrus based on the segregation of isozymes and RFLPs in an intergeneric cross. Theor Appl Genet 84: 49-56.
Kijas JMH, Fowler JCS & Thomas MR (1995). An evaluation of sequence-tagged microsatellite-site markers for genetic analysis within Citrus and related species. Genome 38: 349-355.
Kijas JMH, Thomas MR, Fowler JCS & Roose ML (1997). Integration of trinucleotide microsatellites into a linkage map of Citrus. Theor Appl Genet 94: 701-706.
Kochert G (1994). RFLP technology. In Phillips RL & Vasil IK (eds.) DNA-Based Markers in Plants, pp 8-38. The Netherlands: Kluwer Academic Publishers.
Lander ES, Green P, Abrahamson J, Barlow A, Daly MJ, Lincoln SE & Newburg L (1987). Mapmaker: An interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics 1: 174-181.
Lander ES & Botstein D (1986). Mapping complex genetic traits in humans: New method using a complex RFLP linkage map. Cold Spring Harbor Symposia on Quantitative Biology 51: 49-62.
Liou PC, Gmitter FG & Moore GA (1996). Characterization of the Citrus genome through analysis of restriction fragment length polymorphisms. Theor Appl Genet 92: 425-435.
Luro F, Laigret F, Lorieux M & Ollitrault P (1996). Citrus genome mapping with molecular markers: two maps obtained by segregation analysis of progeny of one intergeneric cross. Proc Int Soc Citricult 2: 862-866.
Manly KF & Elliot RW (1991). MapManager: A microcomputer program for analysis of data from recombinant inbred strains. Mammalian Genome 1: 123-126.
Mohan M, Nair S, Bhagwat A, Krishna TG, Yano M, Bhatia CR & Sasaki T (1997). Genome mapping, molecular markers and marker- assisted selection in crop plants. Mol Breeding 3: 87-103.
Ruiz C & Asins MJ (2003). Comparison between Poncirus and Citrus genetic linkage maps. Theor Appl Genet 106: 826-836.
Sankar AA & Moore GA (2001). Evaluation of inter-simple sequence repeat analysis for mapping in Citrus and extension of the genetic linkage map. Theor Appl Genet 102: 206-214.
Simone M de, Russo MP, Puelo G, Marsan PA, Lorenzoni C, Marocco A & Recupero GR (1998). Construction of genetic maps for Citrus aurantium and C. latipes based on AFLP, RAPD and RFLP markers. Fruits 53: 383-390.
Stam P (1993). Construction of integrated genetic linkage maps by means of a new computer package: JoinMap. Plant J 3: 739-744.
Stam P & Van Ooijen JW (1995). JoinMap (tm) version 2.0: Software for the calculation of genetic linkage maps. DLO-Centre for Plant Breeding and Reproduction Research. Wageningen: The Netherlands.
Staub JE, Serquen FC & Gupta M (1996). Genetic markers, map construction, and their application in plant breeding. HortScience 31: 729-741.
Suiter KA, Wendel JF & Case JS (1983). Linkage-1: A Pascal computer program for the detection and analysis of genetic linkage. J Hered 74: 203-204.
Tores AM, Mau-Lostovicka T, Williams TE & Soost PK (1985). Segregation distortion and linkage of Citrus and Poncirus isozyme genes. J Hered 76: 289-294.
Tozlu I, Guy CL & Moore GA (1999a). QTL analysis of Na+and Cl- accumulation related traits in an intergeneric BC1 progeny of Citrus and Poncirus under saline and non-saline environments. Genome 42: 692-705.
Tozlu I, Guy CL & Moore GA (1999b). QTL analysis of morphological traits in an intergeneric BC1progeny of Citrus and Poncirus under saline and non-saline environments. Genome 42: 1020-1029.
Weber CA, Moore GA, Deng Z & Gmitter FG (2003). Mapping freeze tolerance quantitative trait loci in a Citrus grandis x Poncirus trifoliata F1pseudo-testcross using molecular markers. J Amer Soc Hort Sci 128: 508-514.