Pınar SÖZER BAHADIR, Fakhra LIAQAT, Rengin ELTEM

Plant growth promoting properties of phosphate solubilizing Bacillus species isolated from the Aegean Region of Turkey

Bacillus species, due to their soil amendment properties, are important members of plant-growth-promoting rhizobacteria (PGPR). In this study, with the aim to discover potential biofertilizers, 440 Bacillus isolates from different sources were screened qualitatively for phosphate solubilizing and positive isolates were processed for quantitative estimation of solubilized phosphate and organic acid production. Organic acid production was initially detected by pH change of the media, whereas further confirmation and quantitative estimation were done by gas chromatography (GC). The results indicate that phosphate solubilization ranges from 6.9 ± 1.00 to 95.5 ± 1.83 µg mL−1 for Bacillus isolates and most of the isolates were able to produce more than one organic acid in substantial quantities range between 70.70 ± 1.90 and 619.20 ± 1.40 (ng µL−1). Correlations among total organic acid concentration, final pH of the media, and soluble phosphate were statistically calculated. The six best phosphate-solubilizing isolates were further tested for indole-3-acetic acid (IAA) production, molecular identification, in vitro seed germination, and pot trials. All six strains produced IAA, significantly enhanced radicle and hypocotyl development, and considerably increased plant growth by improving growth of roots and stems. On the basis of results, these Bacillus strains can be considered as potential biofertilizers.

Keywords:

Phosphate solubilization, organic acids indole-3-acetic acid (IAA), pot trials, biofertilizer,

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Asghar HN, Zahir ZA, Arshad M, Khaliq A (2002). Relationship between in vitro production of auxins by rhizobacteria and their growth promoting activities in Brassica juncea L. Biol Fert Soils 35: 231-237.
Banik S, Dey BK (1982). Available phosphate content of an alluvial soil is influenced by inoculation of some isolated phosphate solubilizing-microorganisms. Plant Soil 69: 353-364.
Behera BC, Singdevsachan SK, Mishra RR, Dutta SK, Thatoi HN (2014). Diversity, mechanism and biotechnology of phosphate solubilising microorganism in mangrove - A review. Biocatalysis and Agricultural Biotechnology 3: 97-110.
Bhattacharyya PN, Jha DK (2012). Plant growth-promoting rhizobacteria (PGPR): emergence in agriculture. World J Microb Biot 28: 1327-1350.
Cabra Cendales T, Rodríguez González CA, Villota Cuásquer CP, Tapasco Alzate OA, Hernández Rodríguez A (2017). Bacillus effect on the germination and growth of tomato seedlings ( Solanum lycopersicum L). Acta Biolơgica Colombiana 22: 37- 44.
Chauhan H, Bagyaraj DJ, Selvakumar G, Sundaram SP (2015). Novel plant growth promoting rhizobacteria-Prospects and potential. Appl Soil Ecol 95: 38-53.
Chen YP, Rekha PD, Arun AB, Shen FT, Lai WA, Young CC (2006). Phosphate solubilizing bacteria from subtropical soil and their tricalcium phosphate solubilizing abilities. Appl Soil Ecol 34: 33-41.
Datta C, Basu PS (2000). lndole acetic acid production by a Rhizobium species from root nodules of a leguminous shrub Cajanus cojan . Microbiol Res 155: 123-127.
Delvasto P, Valverde A, Ballester A, Munoz JA, Gonzales F, Blazquez ML, Igual JM, Garcia-Balboa C (2008). Diversity and activity of phosphate bioleaching bacteria from a high-phosphorus iron ore. Hydrometallurgy 92: 124-129.
Erturk Y, Ercisli S, Cakmakci R (2012). Yield and growth response of strawberry to plant growth-promoting rhizobacteria inoculation. J Plant Nutr 35: 817-826.
Hayat R, Ali S, Amara U, Khalid R, Ahmed I (2010). Soil beneficial bacteria and their role in plant growth promotion: a review. Ann Microbiol 60: 579-598.
Illmer P, Schinner F (1992). Solubilization of inorganic phosphates by microorganisms isolated from forest soils. Soil Biol Biochem 24: 389-95.
Islam S, Akanda AM, Prova A, Islam MT, Hossain MM (2016). Isolation and identification of plant growth promoting rhizobacteria from cucumber rhizosphere and their effect on plant growth promotion and disease suppression. Front Microbiol 6: 1360. doi: 10.3389/fmicb.2015.01360.
Kaymak HC (2010). Potential of PGPR in agricultural innovations. In: Maheshwari DK, editor. Plant Growth and Health Promoting Bacteria, Microbiology Monographs, Vol. 18. Berlin, Germany: Springer, pp. 45-79.
Khan MS, Zaidi A, Ahmad E (2014). Mechanism of phosphate solubilization and physiological functions of phosphate- solubilizing microorganisms. In: Khan MS, Zaidi A, Musarrat J, editors. Phosphate Solubilising Microorganisms: Principles and Application of Microphos Technology. Cham, Switzerland: Springer International Publishing, pp. 31-62.
Kumar A, Prakash A, Johri BN (2011). Bacillus as PGPR in crop ecosystem. In: Maheshwari DK, editor. Bacteria in Agrobiology: Crop Ecosystems. Berlin, Germany: Springer, pp. 37-59.
Lamsal K, Kim SW, Kim YS, Lee YS (2012). Application of rhizobacteria for plant growth promotion effect and biocontrol of anthracnose caused by Colletotrichum acutatum on pepper. Mycobiology 40: 244-251.
Lane DJ (1991). 16S/23S rRNA sequencing. In: Stackebrandt E, Goodfellow M, editors. Nucleic Acid Techniques in Bacterial Systematic. New York, NY, USA: John Wiley and Sons, pp. 115- 175.
Liaqat F, Eltem R (2016). Identification and characterization of endophytic bacteria isolated from in vitro cultures of peach and pear rootstocks. 3 Biotech 6: 120-127.
Maitra N, Bandopadhyay C, Samanta S, Sarkar K, Sharma AP, Manna KS (2015). Isolation, identification and efficacy of inorganic phosphate solubilizing bacteria from oxbow lakes of West Bengal, India. Geomicrobiol J 32: 751-758.
Malboobi MA, Owlia P, Behbahani M, Sarokhani E, Moradi S, Yakhchali B, Deljou A, Heravi KM (2009). Solubilization of organic and inorganic phosphates by three highly efficient soil bacterial isolates. World J Microb Biot 25: 1471-1477.
Mehta P, Walia A, Kulshrestha S, Chauhan A, Shirkot CK (2015). Efficiency of plant growth-promoting P-solubilizing Bacillus circulans CB7 for enhancement of tomato growth under net house conditions. J Basic Microb 55: 33-44.
Mena-Violante HG, Olalde-Portugal V (2007). Alteration of tomato fruit quality by root inoculation with plant growth- promoting rhizobacteria (PGPR): Bacillus subtilis BEB-13bs. Sci Hortic- Amsterdam 113: 103-106.
Mohite B (2013). Isolation and characterization of indole acetic acid (IAA) producing bacteria from rhizospheric soil and its effect on plant growth. J Soil Sci Plant Nut 13: 638-649.
Murphy J, Riley JP (1962). A modified single solution method for the determination of phosphate in natural waters. Anal Chim Acta 27: 31-36.
Nautiyal CS (1999). An efficient microbiological growth medium for screening phosphate solubilizing microorganisms. FEMS Microbiol Lett 170: 265-270.
Ng LC, Sariah M, Sariam O, Radziah O, Zainal Abidin MA (2012). Rice seed bacterization for promoting germination and seedling growth under aerobic cultivation system. Aust J Crop Sci 6: 170-175.
Orhan E, Esikten A, Ercisli S, Turan M, Sahin F (2006). Effects of plant growth promoting rhizobacteria (PGPR) on yield growth and nutrient contents in organically growing raspberry. Sci Hortic-Amsterdam 111: 38-43.
Oufdou K, Bechtaoui N, El Alaoui A, Benidire L, Daoui K, Göttfert M (2016). Symbiotic rhizobacteria for improving of the agronomic effectiveness of phosphate fertilizers. Procedia Engineering 138: 325-331.
Park JH, Bolan N, Megharaj M, Naidu R (2011). Isolation of phosphate solubilizing bacteria and their potential for lead immobilization in soil. J Hazard Mater 185: 829-836.
Podile AR, Dube HC (1988). Plant growth-promoting activity of Bacillus subtilis AF1. Curr Sci India 57: 183-186.
Prabakaran, G, Balaraman K, Hoti SL, Manonmani, AM (2007). A cost-effective medium for the large-scale production of Bacillus sphaericus H5a5b (VCRC B42) for mosquito control. Biol Control 41: 379-383.
Rashid M, Khalil S, Ayub N, Alam S, Latif F (2004). Organic acids production and phosphate solubilization by phosphate solubilizing microorganisms (PSM) under in vitro conditions. Pakistan Journal of Biological Sciences 7: 187-196.
Rodr íguez H, Fraga R (1999). Phosphate solubilizing bacteria and their role in plant growth promotion. Biotechnol Adv 17: 319- 339.
Sarkar A, Islam T, Biswas GC, Alam S, Hossain M, Talukder NM (2012). Screening for phosphate solubilizing bacteria inhabiting the rhizoplane of rice grown in acidic soil in Bangladesh. Acta Microbiol Imm H 59: 199-213.
Sharma SB, Sayyed RZ, Trivedi MH, Gobi TA (2013). Phosphate solubilizing microbes: sustainable approach for managing phosphorus deficiency in agricultural soils. SpringerPlus 2: 587. doi: 10.1186/2193-1801-2-587.
Teymouri M, Akhtari J, Karkhane M, Marzban A (2016). Assessment of phosphate solubilization activity of Rhizobacteria in mangrove forest. Biocatalysis and Agricultural Biotechnology 5: 168-172.
Vassilev N, Mendes G, Costa M, Vassileva M (2014). Biotechnological tools for enhancing microbial solubilization of insoluble inorganic phosphates. Geomicrobiol J 31: 751-763.
Vazquez P, Holguin G, Puente ME, Lopez-Cortes A, Bashan Y (2000). Phosphate-solubilizing microorganisms associated with the rhizosphere of mangroves in a semiarid coastal lagoon. Biol Fert Soils 30: 460-468.
Whitelaw MA (1999). Growth promotion of plants inoculated with phosphate-solubilizing fungi. Adv Agron 69: 99-151.
Zahid M, Abbasi MK, Hameed S, Rahim N (2015). Isolation and identification of indigenous plant growth promoting rhizobacteria from Himalayan region of Kashmir and their effect on improving growth and nutrient contents of maize ( Zea mays L.). Front Microbiol 6: 207.
Zaidi A, Khan MS, Ahemad M, Oves M, Wani PA (2009). Recent advances in plant growth promotion by phosphate-solubilizing microbes. In: Khan MS, Zaidi A, Musarrat J, editors. Microbial Strategies for Crop Improvement. 1st ed. Berlin, Germany: Springer, pp. 23-50.
Zaidi A, Khan MS, Ahmad E, Saif S, Rizvi A, Shahid M (2016). Growth stimulation and management of diseases of ornamental plants using phosphate solubilizing microorganisms: current perspective. Acta Physiol Plant 38: 117.