Natalia MILER, İwona JĘDRZEJCZYK

3830

Chrysanthemum plants regenerated from ovaries: a study on genetic and phenotypic variation

Chrysanthemum (Chrysanthemum × grandiflorum /Ramat./Kitam.) is the second most popular ornamental plant in the global flower industry, and there is still a demand for novelty, which forces breeders to search for new sources of variation. The aim of the present study was the evaluation of phenotypic as well as genetic variation of chrysanthemum plants regenerated from ovaries in vitro. In the first vegetative season of plants evaluated in the glasshouse, nine phenotypic variants (16.36%) and 46 (83.6%) true-totype plants were observed. The variation included variegated, marble-like, and lighter-green leaves, and changes in the morphology of inflorescences and ligulate florets, as well as changes in the shape of corymb. Variants with variegated and marble-like leaves were unstable. All 55 regenerants had the same ploidy level (2n = 6x) as control plants, estimated by flow cytometry. Genetic analysis based on RAPD-PCR revealed genetic distances ranging from 0.93% to 7.69% between variants and control plants. It was concluded that variable regenerants did not originate from the gynogenic pathway, but they regenerated from somatic tissue and underwent somaclonal variation.
Keywords:

Genetic distance, ploidy level RAPD, somaclonal variation,

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  • Anderson NO (2007). Chrysanthemum. In: Anderson NO, editor. Flower Breeding and Genetics. Berlin, Germany: Springer Science + Business Media B.V., pp. 389-437.
  • Bohanec B (2009). Doubled haploids via gynogenesis. In: Touraev A, Forster BP, Jain SM, editors. Advances in Haploid Production in Higher Plants. Berlin, Germany: Springer Science + Business Media B.V., pp. 35-46.
  • Chakrabarty D, Datta SK (2010). Management of chimera and in vitro mutagenesis for development of new flower colour/shape and chlorophyll variegated mutants in chrysanthemum. In: Datta SK, Chakrabarty D, editors. Floriculture: Role of Tissue Culture and Molecular Techniques. Jaipur, India: Pointer Publishers, pp. 157-164.
  • Chen JF, Cui L, Malik AA, Mbira KG (2011). In vitro haploid and dihaploid production via unfertilized ovule culture. Plant Cell Tiss Org 104: 311-319.
  • Datta SK (2013). Chrysanthemum morifolium Ramat. A unique genetic material for breeding. Science and Culture 7-8: 307- 313.
  • Drewlow LW, Ascher PD, Widmer RE (1973). Rapid method of determining pollen incompatibility in Chrysanthemum morifolium Ramat. Theor Appl Genet 43: 1-5.
  • Feng SG, He RF, Jiang MY, Lu JJ, Shen XX, Liu JJ, Wang ZA, Wang HZ (2016). Genetic diversity and relationships of medicinal Chrysanthemum morifolium revealed by start codon targeted (SCoT) markers. Sci Hortic-Amsterdam 201: 118-123.
  • Ferrie AMR (2012). Doubled haploidy as a tool in ornamental breeding. Acta Hortic 953: 167-171.
  • Galbraith D W, Harkins KR, Maddox JM, Ayres NM, Sharma DP, Firoozabady E (1983). Rapid flow cytometric analysis of the cell cycle in intact plant tissues. Science 220: 1049-1051.
  • Jain MS (2001). Tissue culture-derived variation in crop improvement. Euphytica 118: 153-166.
  • Karp A (1995). Somaclonal variation as a tool for crop improvement. Euphytica 85: 295-302.
  • Kengkarj P, Smitamana P, Fujime Y (2008). Assessment of somaclonal variation in chrysanthemum ( Dendranthema grandiflora Kitam.) using RAPD and morphological analysis. Plant Tissue Culture and Biotechnology 18: 139-149.
  • Klie M, Schie S, Linde M, Debener T (2014). The type of ploidy of Chrysanthemum is not black or white: a comparison of a molecular approach to published cytological methods. Front Plant Sci 479: 1-8.
  • Leal F, Loureiro J, Rodriguez E, Pais MS, Santos C, Pinto-Carnide O (2006). Nuclear DNA content of Vitis vinifera cultivars and ploidy level analyses of somatic embryo-derived plants obtained from anther culture. Plant Cell Rep 25: 978-985.
  • Miler N, Muszczyk P (2015). Regeneration of callus and shoots from the ovules and ovaries of chrysanthemum in vitro . Acta Hortic 1083: 103-106.
  • Miler N, Zalewska M (2014). Somaclonal variation of chrysanthemum propagated in vitro from different explant types. Acta Sci Pol- Hortoru 13: 69-82.
  • Miňano HS, Gonzales-Benito ME, Martin C (2009). Molecular characterization and analysis of somaclonal variation in chrysanthemum cultivars using RAPD markers. Sci Hortic 122: 238-243.
  • Murashige T, Skoog F (1962). A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol Plantarum 15: 473-497.
  • Nei M, Li WS (1979). Mathematical model for studying genetic variation in terms of restriction nucleases. P Natl Acad Sci USA 76: 5269-5273.
  • Newbury HJ, Ford-Lloyd BV (1993). The use of RAPD for assessing variation in plants. Plant Growth Regul 12: 43-51.
  • Olszewska D, Jędrzejczyk I, Nowaczyk P, Sendel S, Gaczkowska B (2015). In vitro colchicine treatment of anther-derived pepper haploids. Bulg J Agric Sci 21: 806-810.
  • Royal Horticultural Society Colour Chart (1967). London, UK: Royal Horticultural Society. Schum A (2003). Mutation breeding in ornamentals: an efficient breeding method? Acta Hortic 612: 47-60.
  • Taixeira da Silva JA, Lema-Rumińska J, Tymoszuk A, Kulpa D (2015). Regeneration from chrysanthemum flowers: a review. Acta Physiol Plant 37: 36.
  • Tymoszuk A, Zalewska M (2014). Biological factors affecting regeneration of adventitious shoots from in vitro isolated ligulate florets of chrysanthemum. Acta Sci Pol-Hortoru 13: 155-165.
  • Van de Peer Y, De Watcher Y (1994). TREECON for Windows: a software package for the construction and drawing of evolutionary trees for the Microsoft Windows environment. Computer Applications in the Biosciences 10: 569-570.
  • Wang H, Dong B, Jiang J, Fang W, Guan Z, Liao Y, Chen S, Chen F (2014a). Characterization of in vitro haploid and doubled haploid Chrysanthemum morifolium plants via unfertilized ovule culture for phenotypic traits and DNA methylation pattern. Front Plant Sci 738: 1-10.
  • Wang F, Zhang F, Chen F, Fang W, Teng N (2014b). Identification of chrysanthemum ( Chrysanthemum morifolium ) self- incompatibility. Sci World J article ID 625658, 9 pages. Watanabe K (1977). Successful ovary culture and production of F 1 hybrids and androgenic haploids in Japanese Chrysanthemum species. J Hered 68: 317-320.
  • Welsh J, McClelland M (1990). Fingerprinting genomes using PCR with arbitrary primers. Nucleic Acids Res 18: 7213-7218.
  • Williams JGK, Kubelik AR, Livak JL, Rafalski JA, Tingey SV (1990). DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res 18: 6531-6535.
Turkish Journal of Botany-Cover
  • ISSN: 1300-008X
  • Yayın Aralığı: Yılda 6 Sayı
  • Yayıncı: TÜBİTAK
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