Two new species found in Northwestern Pakistan in the mushroom genus Amanita are described and illustrated. Phylogenetic data derived from nuclear ribosomal ITS and LSU regions along with morphological characterizations indicate that these species are novel. Amanita cinis is a member of section Roanokenses, while A. olivovaginata is representative of the section Vaginatae. Amanita emodotrygon, recently described from the state of Uttarakhand, India, is new to Pakistan.