Beren KARAOSMANOĞLU, Ekim Zihni TAŞKIRAN

Transcriptome analysis reveals differentially expressed genes between human primary bone marrow mesenchymal stem cells and human primary dermal fibroblasts

: Stromal cells have been widely used in biomedical research and disease modeling studies in vitro. The most commonly usedtypes of stromal cells are mesenchymal stem cells and fibroblasts. Their cellular phenotypes and differentiation capabilities are quitesimilar and there are no specific distinction criteria. In order to identify transcriptomic differences between these 2 cell types, weperformed next-generation RNA sequencing. Using the global gene expression profile and pathway analysis, we showed that humanprimary bone marrow mesenchymal stem cells and human primary dermal fibroblasts have different molecular signatures. We alsoidentified critical transcription factors that are differentially expressed between these cells. We then proposed that homeobox genes andsome other sequence-specific transcription factors are not only responsible for transcriptional differences, but also discriminate bonemarrow mesenchymal stem cells and dermal fibroblasts at the transcriptional level.

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