Genome and metabolome mining of marine obligate Salinispora strains to discover new natural products

Marine microorganisms are receiving more attention as a promising potential source of new natural products. In the presentstudy, we performed genomic and metabolomic analyses to explore the metabolic potential of the obligate marine actinomycete genusSalinispora. The genomes of thirty Salinispora strains were prospected in search of biosynthetic gene clusters including polyketidesynthase (PKS), nonribosomal peptide synthetase (NPRS), terpene, indole, lantibiotics, and siderophores. We determined considerablediversity of natural product biosynthetic gene clusters in their genome. There were a total of 1428 putative gene clusters involved in thebiosynthesis of various bioactive natural products. Furthermore, 1509 ketosynthase (KS) and condensation (C) domains were detectedby using NapDoS belonging to PKS and NRPS genes, respectively. Metabolic profiling was performed by a nontargeted LC-MS/MSapproach combined with spectral networking using Global Natural Product Social Molecular Networking (GNPS). Dereplication andtentative identification of natural products were evaluated for common chemical properties and their associated pathways. Significantbioactive natural products such as lomaiviticin C, 7-OH-staurosporine, staurosporine, and cyanosporaside B were determined. Moreimportantly, an unknown glycosylated compound associated with an NRPS/PKS-I hybrid gene cluster in Salinispora pacifica CNY703was established through chemical and genomic analyses.

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