Prediction and expression analysis of G protein-coupled receptors in the laboratory stick insect, Carausius morosus
Prediction and expression analysis of G protein-coupled receptors in the laboratory stick insect, Carausius morosus
G protein-coupled receptors (GPCRs) are 7-transmembrane proteins that transduce various extracellular signals intointracellular pathways. They are the major target of neuropeptides, which regulate the development, feeding behavior, mating behavior,circadian rhythm, and many other physiological functions of insects. In the present study, we performed RNA sequencing and denovo transcriptome assembly to uncover the GPCRs expressed in the stick insect Carausius morosus. The transcript assemblies werepredicted for the presence of 7-transmembrane GPCR domains. As a result, 430 putative GPCR transcripts were obtained and 43 ofthese revealed full-length sequences with highly significant similarity to known GPCR sequences in the databases. Thirteen differentGPCRs were chosen for tissue expression analysis. Some of these receptors, such as calcitonin, inotocin, and tyramine receptors, showedspecific expression in some of the tissues. Additionally, GPCR prediction yielded a novel uncharacterized GPCR sequence, which wasspecifically expressed in the central nervous system and ganglia. Previously, the only information about the anatomy of the stick insectwas on its gastrointestinal system. This study provides complete anatomical information about the adult insect.
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- Andrews S. (no date) FastQC, Babraham Bioinformatics. Available at:
https://www.bioinformatics.babraham.ac.uk/projects/fastqc/.
Audsley N, Weaver RJ (2009). Neuropeptides associated with the
regulation of feeding in insects. Gen Comp Endocr 162: 93-104.
doi: 10.1016/j.ygcen.2008.08.003.
- Bhasin M, Raghava GPS (2004). GPCRpred: an SVM-based method
for prediction of families and subfamilies of G-protein coupled
receptors. Nucleic Acids Res 32: 383-389. doi: 10.1093/nar/
gkh416.
Bläsing B, Cruse H (2004). Mechanisms of stick insect locomotion in
a gap-crossing paradigm. J Comp Physiol A 190: 173-183. doi:
10.1007/s00359-003-0482-3.
- Buckley SJ, Fitzgibbon QP, Smith GG, Ventura T (2016). In silico
prediction of the G-protein coupled receptors expressed during
the metamorphic molt of Sagmariasus verreauxi (Crustacea:
Decapoda) by mining transcriptomic data: RNA-seq to
repertoire. Gen Comp Endocr 228: 111-127. doi: 10.1016/j.
ygcen.2016.02.001.
- Chérasse S, Aron S (2017). Measuring inotocin receptor gene
expression in chronological order in ant queens. Horm Behav
doi: 10.1016/j.yhbeh.2017.09.009.
- Dahl SG, Sylte I, Ravna AW (2004). Structures and models of
transporter proteins. J Pharmacol Exp Ther 309: 853-860. doi:
10.1124/jpet.103.059972.
- Dallmann CJ, Hoinville T, Dürr V, Schmitz J (2017). A load-based
mechanism for inter-leg coordination in insects. P Roy Soc
B-Biol Sci 284(1868). doi: 10.1098/rspb.2017.1755.
- Duan Sahbaz B, Sezerman OU, Torun H, Birgül Iyison N (2017).
Ligand binding pocket of a novel Allatostatin receptor type C
of stick insect, Carausius morosus. Sci Rep-UK 7. doi: 10.1038/
srep41266.
- El-Kholy S, Stephano F, Li Y, Bhandari A, Fink C, Roeder T (2015).
Expression analysis of octopamine and tyramine receptors in
Drosophila. Cell Tissue Res 361: 669-684. doi: 10.1007/s00441-
015-2137-4.
- Gasteiger E, Gattiker A, Hoogland C, Ivanyi I, Appel RD, Bairoch
A (2003). ExPASy: the proteomics server for in-depth protein
knowledge and analysis. Nucleic Acids Res. doi: 10.1093/nar/
gkg563.
- Grabherr MG, Haas BJ, Yassour M, Levin JZ, Thompson DA, Amit
I, Adiconis X, Fan L, Raychowdhury R, Zeng Q et al. (2013).
Trinity: reconstructing a full-length transcriptome without a
genome from RNA-Seq data. Nat Biotechnol 29: 644-652. doi:
10.1038/nbt.1883.Trinity.
- Gruhn M, Rosenbaum P, Bockemühl T, Büschges A (2016). Body
side-specific control of motor activity during turning in a
walking animal. eLife doi: 10.7554/eLife.13799.
- Guerrero FD, Kellogg A, Ogrey AN, Heekin AM, Barrero R, Bellgard
MI, Dowd SE, Leung MY (2016). Prediction of G proteincoupled receptor encoding sequences from the synganglion
transcriptome of the cattle tick, Rhipicephalus microplus. Ticks
Tick-borne Dis. doi: 10.1016/j.ttbdis.2016.02.014.
- Hanin O, Azrielli A, Zakin V, Applebaum S, Rafaeli A (2011).
Identification and differential expression of a sex-peptide
receptor in Helicoverpa armigera. Insect Biochem Molec. doi:
10.1016/j.ibmb.2011.03.004.
- Hemminger W, Sarge SM, Bundesanstalt P (1998). Post-Targeting
Functions of Signal Peptides. Protein Transport into the
Endoplasmic Reticulum. doi: 10.1007/BFb0111316.
- Jang YH, Chae HS, Kim YJ (2017). Female-specific myoinhibitory
peptide neurons regulate mating receptivity in Drosophila
melanogaster. Nat Commun doi: 10.1038/s41467-017-01794-9.
- Kokot F, Ficek R (1999). Effects of neuropeptide Y on appetite. Miner
Electrol Metab 25: 303-305. doi: 10.1159/000057464.
- Krogh A, Larsson B, von Heijne G, Sonnhammer EL (2001).
Predicting transmembrane protein topology with a hidden
Markov model: application to complete genomes. J Mol Biol
305: 567-580. doi: 10.1006/jmbi.2000.4315.
- Liesch J, Bellani LL, Vosshall LB (2013). Functional and genetic
characterization of neuropeptide Y-like receptors in Aedes
aegypti. PLoS Neglect Trop D 7(10). doi: 10.1371/journal.
pntd.0002486.
- Liessem S, Ragionieri L, Neupert S, Büschges A, Predel R (2018).
Transcriptomic and neuropeptidomic analysis of the stick
insect, Carausius morosus. J Proteome Res 17: 2192-2204. doi:
10.1021/acs.jproteome.8b00155.
- Marco HG, Katali OKH, Gäde G (2018). Influence of aminergic and
peptidergic substances on heart beat frequency in the stick
insect Carausius morosus (Insecta, Phasmatodea). Arch Insect
Biochem. doi: 10.1002/arch.21469.
- Min XJ, Butler G, Storms R, Tsang A (2005). OrfPredictor: predicting
protein-coding regions in EST-derived sequences. Nucleic
Acids Res 33(SUPPL. 2): 677-680. doi: 10.1093/nar/gki394.
- Monastirioti M (2003). Distinct octopamine cell population residing
in the CNS abdominal ganglion controls ovulation in Drosophila
melanogaster. Dev Biol doi: 10.1016/j.ydbio.2003.07.019.
- Paluzzi JP, Park Y, Nachman RJ, Orchard I (2010). Isolation,
expression analysis, and functional characterization of the first
antidiuretic hormone receptor in insects. Proc Natl Acad Sci U
S A 107: 10290-10295. doi: 10.1073/pnas.1003666107.
- Pándy-Szekeres G, Munk C, Tsonkov TM, Mordalski S, Harpsøe
K, Hauser AS, Bojarski AJ, Gloriam DE (2018). GPCRdb in
2018: adding GPCR structure models and ligands. Nucleic
Acids Res. doi: 10.1093/nar/gkx1109.
- Regard JB, Sato IT, Coughlin SR (2009). Anatomical profiling of G
protein-coupled receptor expression. Cell 135: 561-571. doi:
10.1016/j.cell.2008.08.040.
- Schultz J, Milpetz F, Bork P, Ponting CP (1998). SMART, a
simple modular architecture research tool: identification of
signaling domains. Proc Natl Acad Sci U S A. doi: 10.1073/
pnas.95.11.5857.
- Screpanti E, Hunte C (2007). Discontinuous membrane helices in
transport proteins and their correlation with function. J Struct
Biol 159: 261-267. doi: 10.1016/j.jsb.2007.01.011.
- Secher T, Lenz C, Cazzamali G, Sørensen G, Williamson M, Hansen
GN, Svane P, Grimmelikhuijzen CJ (2001). Molecular
cloning of a functional allatostatin gut/brain receptor and an
allatostatin preprohormone from the silkworm Bombyx mori.
J Biol Chem 276: 47052-47060. doi: 10.1074/jbc.M106675200.
- Shelomi M, Sitepu IR, Boundy-Mills KL, Kimsey LS (2015).
Review of the gross anatomy and microbiology of the
Phasmatodea digestive tract. J Orthoptera Res 24: 29-40. doi:
10.1665/034.024.0105.
- Wadsworth T, Carriman A, Gutierrez AA, Moffatt C, Fuse M (2014).
Ecdysis behaviors and circadian rhythm of ecdysis in the stick
insect, Carausius morosus. J Insect Physiol. doi: 10.1016/j.
jinsphys.2014.10.003.
- Wistrand M, Käll L, Sonnhammer ELL (2006). A general model of
G protein-coupled receptor sequences and its application to
detect remote homologs. Protein Sci 15: 509-521. doi: 10.1110/
ps.051745906.
- Xu G, Gu GX, Teng ZW, Wu SF, Huang J, Song QS, Ye GY, Fanga Q
(2016). Identification and expression profiles of neuropeptides
and their G protein-coupled receptors in the rice stem borer
Chilo suppressalis. Sci Rep-UK 6. doi: 10.1038/srep28976.
- Yamanaka N, Hua YJ, Roller L, Spalovská-Valachová I, Mizoguchi
A, Kataoka H, Tanaka Y (2010). Bombyx prothoracicostatic
peptides activate the sex peptide receptor to regulate
ecdysteroid biosynthesis. Proc Natl Acad Sci U S A. doi:
10.1073/pnas.0907471107.
- · Zandawala M, Li S, Hauser F, Grimmelikhuijzen CJP, Orchard I
(2013). Isolation and functional characterization of calcitoninlike diuretic hormone receptors in Rhodnius prolixus. PLoS
ONE. doi: 10.1371/journal.pone.0082466.