The use of random amplified polymorrphic DNA (RAPD) marrkers in sex discrimination in nile tilapia, oreochromis niloticus (Pisces: cichlidae)

Rastgele çogaltılmıs polimorfik DNA (RAPD) markerleri kullanarak lineer ayrıcı fonksiyon analizi, Nil tilapia balıgının (Oreochromis niloticus) cinsiyetlerini ayırmada basarılı olarak kullanıldı. Sonuçlar tilapiada ana genetiksel cinsiyet belirleme faktörlerinin oldugu görüsüne destek saglamaktadır.

Nil Tilapia Balığının (Pisces. cichlidae) cinsiyet ayırımında rastgele coğaltılmış polimorfik DNA (RAPD) markerlerinin kullanımı

Random amplified polymorphic DNA (RAPD) markers were successfully used in discrimination of sexes in Nile tilapia fish ( Oreochromis niloticus) using linear discriminant function analysis. The results provide support for the view that major genetical sex determining factors exist in tilapia.

___

  • 1. Bull J.J., Evolution of sex determining mechanisms. Benjamin/Cummings Inc., Menlo Park, CA, 1983. 2. Kirpichnikov V.S., Genetic Basis of fish selection. Springer-Verlag, Heidelberg, 1981 3. Thorgaard G.H., Heteromorphic sex chromosomes in male rainbow trout. Science 196, 900-902, 1977. 4. Lloyd M.A. and Thorgaard G.H., Restriction endonuclease banding of rainbow trout chromosomes. Chromosoma 96, 171-177, 1988. 5. Reed K.M. and Phillips R.B., Molecular characterization and cytogenetic analysis of highly repeated DNAs of lake trout, Salvelinus namaycush. Chromosoma 104, 242-251, 1995. 6. Nanda I.W., Feichtinger W., Schmid M., Schroeder J.H., Zischler H. and Epplen J.T. Simple repetitive sequences are associated with differentiation of the sex chromosomes in the guppy fish. J. Mol. Evol. 30, 456-462, 1990.
  • 7. Devlin R.H., McNeil B.K., Groves, T.D.D., Donaldson, E.M., Isolation of a Y-chromosomal DNA probe capable of determining genetic sex in chinook salmon (Oncorhynchus tshawytscha). Can. J. Fish. Aquat. Sci. 48, 1606-1612, 1991. 8. Forbes S.H., Knudson K.L., North T.W. and Allendorf F.W., One of two growth hormone genes in coho salmon is sex-linked. Proc. Natl. Acad. Sci. USA 91, 1628-1631, 1994. 9. Nakayama I., Foresti F., Tewari R., Schartl M. and Chourrout D., Sex chromosome polymorphism and heterogametic males revealed by two cloned DNA probes in the ZW/ZZ fish Lepomis elongatus, Chromosoma, 103, 31-39, 1994. 10. Wohlfarth, G.W. and Wedekind, H., The heredity of sex determination in tilapias. Aquaculture, 92, 143-156, 1991. 11. Mair, G.C., Chromosome-set manipulation in tilapia- techniques, problems and prospects. Aquaculture, 111, 227- 244, 1993. 12. Trombka, D. and Avtalion, R., Sex determination in tilapia - a review. The Israeli Journal of Aquaculture-Bamidgeh, 45, 26-37, 1993. 13. Kornfield, I.L., Descriptive genetics of Cichlid fishes. In Evolutionary Genetics of Fishes (ed. by B.J. Turner ), New York, 1984, Plenum Publ. Corp., pp. 519-516. 14. Majumdar, K.C. and McAndrew, B.J., Relative DNA content of somatic cell nuclei and chromosomal studies in three genera, Tilapia, Sarotherodon and Oreochromis of the tribe Tilapiini. Genetica, 68, 165-168, 1986. 15. Crosetti, D., Sola, L., Brunner, P. and Cataudella, S. (1988). Cytogenetical characterization of O. niloticus and O. mossambicus and their hybrid. In 2nd Int. Symp. on Tilapia in Aquaculture (ed. by R.S.V. Pullin, T. Bhukaswan, K. Tanguthai, and J.L. Maclean), 1988, ICLARM Conf Proc., pp. 143-151. 16. McConnel, S.K.J., Studies on the Molecular Biology of Sex Determination in the Tilapias. PhD. Thesis. University of Wales, Swansea, 1993. 17. Williams, J.G.K., Kubelik, A.R., Livak, K.J., Rafalski, J.A. and Tingey, S.V., DNA polymorphisms by arbitrary primers are useful as genetic markers. Nucleic Acids Res., 18, 6531-6535, 1990. 18. Bardakci, F. and Skibinski, D.O.F., Applications of the RAPD technique in tilapia fish: species and subspecies identification. Heredity, 73, 117-123, 1994. 19. Mair, G.C., Penman, D.J., Scott, A., Skibinski, D.O.F. and Beardmore, J.A. , Hormonal sex-reversal and the mechanism of sex determination in Oreochromis. In Proc. World Symp. on Hybridization and Genetic Engineering in Aquaculture (ed. by K. Tiews), 1987, Bordeaux, France, pp. 301-312. 20. Michelmore R.W., Paran I. and Kesseli R.V., Identification of markers linked to disease- resistance genes by bulked segregant analysis: A rapid method to detect markers in specific genomic regions by using segregating populations. Proc. Natl. Acad. Sci. 88, 9828-9832, 1991. 21. Levin, I., Crittenden, L.B., and Dodgson, J.B., Genetic map of the chicken Z chromosome using random amplified polymorphic DNA (RAPD) markers. Genomics, 16, 224-230, 1993. 22. Hormaza, J.I., Dollo, L. and Polito, V.S., Identification of a RAPD marker linked to sex determination in Pistacia vera, using bulked segregant analysis. Theor. Appl. Genet., 89, 9-13, 1994.
  • 23. Mair, G.C., Scott, A.G., Penman, D.J., Beardmore, J.A. and Skibinski, D.O.F., Sex determination in the genus Oreochromis. 1. sex reversal, gynogenesis and triploidy in O. niloticus (L.). Theor. Appl. Genet., 82, 144-152, 1991. 24. Baroiller J.F., Clota F. and Geraz E. (1995). Temperature sex determination in two tilapia, Oreochromis niloticus and red tilapia (red Florida strain): effect of high or low temperature. In: Proc. of the fifth int. symp. on the reproductive physiology of fish (ed. by F.W. Goetz and P Thomas), Austin TX, 1995, pp. 158-160.