Melek KÜÇÜKOĞLU, Uğur Seçil BİNOKAY, Ayper Boğa PEKMEZEKMEK

The effects of zinc chloride during early embryonic development in zebrafish (Brachydanio rerio)

This study investigated the developmental toxicity of zinc chloride (ZnCl2) in zebrafish embryos (Brachydanio rerio, Cyprinidae, Cypriniformes). Zebrafish embryos were exposed to 5 different concentrations of ZnCl2, from the blastula stage, for 15 days under static renewal test conditions. As a result, the corresponding median lethal concentration (LC50) value determined for ZnCl2 exposure was 1.36 mg/L (0.65 mg/L as a lone Zn2+ ion). At 1.0 mg/L ZnCl2, the exposed group's hatching began at 7 days instead of at 4 days, and most of the embryos died in the chorion without hatching at 11 and 12 days. Developmental deformities such as abnormal embryogenesis, low hatchability, delayed hatching, and reduction of newly hatched larvae, and a poor survival ratio (mortality ratio of 1.5 and 10 mg/L concentrations compared to control, P < 0.001), were observed during the embryo larval stage due to zinc exposure. Based on these results, we observed that critical and teratogenic effects of ZnCl2 on embryonic development of zebrafish occurred at concentrations greater than 0.5 mg/L. Moreover, our results confirm that the zebrafish embryo teratogenesis assay can be a useful pretest for integrated biological hazard assessment of chemical agents used in industrial production and drug development technologies.

Anahtar Kelimeler:

Key words: Embryonic development, teratogenicity, zinc chloride, zebrafish, toxicity

The effects of zinc chloride during early embryonic development in zebrafish (Brachydanio rerio)

Keywords:

Key words: Embryonic development, teratogenicity, zinc chloride, zebrafish, toxicity,

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Scholz S, Fischer S, Gündel U et al. The zebrafish embryo model in environmental risk assessment – applications beyond acute toxicity testing. Environ Sci Pollut Res 15: 394–404, 2008.
Binetti R, Costamagna FM, Marcello I. Exponential growth of new chemicals and evolution of information relevant to risk control. Ann Ist Super Sanità 44: 13–15, 2008.
Lee HY, Inselman AL, Kanungo J et al. Alternative models in developmental toxicology. Syst Biol Reprod Med 58: 10–22, 20 Sabourin TD, Faulk RT, Goss LB. The efficacy of three nonmammalian test systems in the identification of chemical teratogens. J Appl Toxicol 5: 227–233, 1995.
Davis K, Schultz TW, Dumont J. Toxic and teratogenic effects of selected aromatic amines on embryos of the amphibian Xenopus laevis. Arch Environ Contam Toxicol 10: 371–391, 19 Dial NA. Methylmercury: teratogenic and lethal effects in frog embryos. Teratology 13: 327–333, 1976.
Birge WJ, Black JA, Westerman AG. Short term fish and amphibian embryo-larval tests for determining the effects of toxicant stress on early life stages and estimating chronic values for single compounds and complex effluents. Environ Toxicol Chem 4: 807–821, 1985.
McKim JM. Evaluation of tests with early life stages of fish for predicting long-term toxicity. J Fish Res Board Can 34: 1148– 1154, 1977.
Parng C, Seng WL, Semino C et al. Zebrafish: a preclinical model for drug screening. Assay Drug Dev Techn 1: 41–48, 200 Yen J, Donerly S, Levin ED et al. Differential acetylcholinesterase inhibition of chlorpyrifos, diazinon and parathion in larval zebrafish. Neurotoxicol Teratol 33: 735–741, 2011.
Xu Z, Williams FE, Liu MC. Developmental toxicity of dextromethorphan in zebrafish embryos/larvae. J Appl Toxicol 31: 157–163, 2011.
Cowden J, Padnos B, Hunter D et al. Developmental exposure to valproate and ethanol alters locomotor activity and retinotectal projection area in zebrafish embryos. Reprod Toxicol 33: 165–173, 2012.
Shaukat A, Champagne DL, Spaink HP et al. Zebrafish embryos and larvae: a new generation of disease models and drug screens. Birth Defects Res C Embryo Today 93: 115–133, 20 Panzica-Kelly JM, Zhang CX, Danberry TL et al. Morphological score assignment guidelines for the dechorionated zebrafish teratogenicity assay. Birth Defects Res B Dev Reprod Toxicol 89: 382–95, 2010.
Gustafson AL, Stedman DB, Ball J et al. Inter-laboratory assessment of a harmonized zebrafish developmental toxicology assay: progress report on phase I. Reprod Toxicol 33: 155–164, 2012.
Hill AJ, Teraoka H, Heideman W et al. Zebrafish as a model vertebrate for investigating chemical toxicity. Toxicol Sci 86: 6–19, 2005.
McCollum CW, Ducharme NA, Bondesson M et al. Developmental toxicity screening in zebrafish. Birth Defects Res C Embryo Today 93: 67–114, 2011.
Lieschke GJ, Currie PD. Animal models of human disease: zebrafish swim into view. Nat Rev Genet 8: 353–367, 2007.
Stebler EF, Burks SL, Bantle JA et al. Evaluation of the developmental toxicity of metal-contaminated sediments using short-term fathead minnow and frog embryo larval assay. Environ Toxicol Chem 7: 27–34, 1988.
Hisaoka KK, Battle HI. The normal developmental stages of the zebrafish, Brachydanio rerio (Hamilton-Buchanan). J Morphol 102: 311–328, 1958.
Dave G, Damgaard B, Grande M et al. Ring test of an embryo– larval toxicity test with zebrafish using chromium and zinc as toxicants. Environ Toxicol Chem 6: 61–71, 1987.
Birge WJ, Black JA, Westerman AG et al. Fish and amphibian embryos: a model system for evaluating teratogenicity. Toxicol Sci 3: 237–242, 1983.
Nagel R. DarT: The embryo test with the zebrafish Danio rerio – a general model in ecotoxicology and toxicology. ALTEX 1: 38–48, 2002.
Skidmore JF. Resistance to zinc sulphate of the zebrafish (Brachydanio rerio Hamilton-Buchanan) at different phases of its life history. Ann Appl Biol 56: 47–53, 1965.
Huang W, Cao L, Shan X. Toxic effects of zinc on the development, growth, and survival of red sea bream Pagrus major embryos and larvae. Arch Environ Contam Toxicol 58: 140–150, 2010.
Güner U. Heavy metal effects on P, Ca, Mg, and total protein contents in embryonic pleopodal eggs and stage-1 juveniles of freshwater crayfish Astacus leptodactylus (Eschscholtz, 1823). Turk J Biol 34: 405–412, 2010.
Leblebici Z, Aksoy A, Duman F. Influence of salinity on the growth and heavy metal accumulation capacity of Spirodela polyrrhiza (Lemnaceae). Turk J Biol 35: 215–220, 2011.
Boztepe H. Inorganic Chemistry. Çukurova University Press. Adana, Turkey; 1987.
Brannen KC, Panzica-Kelly JM, Danberry TL et al. Development of a zebrafish embryo teratogenicity assay and quantitative prediction model. Birth Defects Res Part B Dev Reprod Toxicol 89: 66–77, 2010.
Laale HW. The biology and use of zebrafish, Brachydanio rerio in fisheries research. J Fish Biol 10: 121–173, 1977.