Regulation of functional activities and essential oil production in Vetiveria zizanioides L. Nash after γ-irradiated sodium alginate elicitation

Regulation of functional activities and essential oil production in Vetiveria zizanioides L. Nash after γ-irradiated sodium alginate elicitation

Upon irradiation, marine polysaccharides undergo depolymerization, leading to formation of oligosaccharides that elicit various biological activities in plants. Taking a step further on the previously established growth-promoting activity of irradiated sodium alginate (ISA), structural rearrangements in ISA were analyzed using complementary techniques to develop an understanding of the structure property relationship. The essential oil (EO) of vetiver (Vetiveria zizanioides L. Nash) immensely benefits the perfumery industry, proving itself as an economically important crop. A pot experiment was designed to test the effect of water-soluble ISA fractions on the growth, physiology, and EO production of vetiver. The structural characterization of radiation-induced sodium alginate was carried out using SEM, FT-IR, and UV-Vis spectroscopy. Of the various treatments employed, ISA-120 (ISA applied at 120 mg L 1) proved the best for most of the parameters studied, including fresh and dry weight of plants and photosynthetic attributes. As compared to the control (water-spray treatment), foliar feeding of ISA-120 resulted in an increase of 21.2% in chlorophyll content, while this treatment enhanced the chlorophyll fluorescence by 21.8% at 300 days after transplanting. Application of ISA-120 also increased the EO content by 21.1% and EO yield by 47.6%. Gas chromatography revealed an increase of 30.1% and 92.6% in the values of content and yield of khusimol, respectively, over the control.

___

  • Adams PR (2007). Identification of Essential Oil Components by Gas Chromatography/Mass Spectrometry. Carol Stream, IL, USA: Allured Publishing Corporation.
  • Aftab T, Khan MM, Idrees M, Naeem M, Hashmi N, Varshney L (2011). Enhancing the growth, photosynthetic capacity and artemisinin content in Artemisia annua L. by irradiated sodium alginate. Radiat Phys Chem 80: 833-836.
  • Aftab T, Khan MMA, Naeem M, Idrees M, Siddiqi TO, Varshney L (2014). Effect of irradiated sodium alginate and phosphorus on biomass and artemisinin production in Artemisia annua. Carbohyd Polym 110: 396-404.
  • Aftab T, Naeem M, Idrees M, Khan MMA, Moinuddin, Varshney L (2013). Cumulative role of irradiated sodium alginate and nitrogen fertilizer on growth, biochemical processes and artemisinin production in Artemisia annua. Ind Crop Prod 50: 874-881.
  • Aftab T, Naeem M, Idrees M, Khan MMA, Moinuddin, Varshney L (2016). Simultaneous use of irradiated sodium alginate and nitrogen and phosphorus fertilizers enhance growth, biomass and artemisinin biosynthesis in Artemisia annua L. J Appl Res Med Aromat Plants 3: 186-194.
  • Ahmad B, Khan MMA, Jaleel H, Sadiq Y, Shabbir A, Uddin M (2017). Exogenously sourced γ-irradiated chitosan-mediated regulation of growth, physiology, quality attributes and yield in Mentha piperita L. Turk J Biol 41: 388-401.
  • Ahni KJ, Park L, Byun MW (2001). The production of chitosan and alginate monomer and oligomer by irradiation. In: IFT Annual Meeting Session 59A, New Orleans, LA, USA.
  • Ali A, Khan MMA, Moinuddin, Naeem M, Idrees M, Hashmi N, Dar TA, Varshney L (2014). Radiolytically depolymerized sodium alginate improves physiological activities, yield attributes and composition of essential oil of Eucalyptus citriodora Hook. Carbohyd Polym 112: 134-144.
  • Antiochia R, Campanella L, Ghezzi P, Movassaghi K (2007). The use of vetiver for remediation of heavy metal soil contamination. Anal Bioanal Chem 388: 947-956.
  • Avella M, Di Pace E, Immirzi B, Impallomeni G, Malinconico M, Santagata G (2007). Addition of glycerol plasticizer to seaweeds derived alginates: influence of microstructure on chemicalphysical properties. Carbohydr Polym 69: 503-511.
  • Bhushan B, Kumar SS, Tanuja S, Lalit S, Hema A (2013). Vetiveria zizanioides (Linn.) Nash: A pharmacological overview. Int Res J Pharm 4: 18-20.
  • Brandt R, Merkl N, Schultze-Kraft R, Infante C, Broll G (2006). Potential of vetiver (Vetiveria zizanioides (L.) Nash) for phytoremediation of petroleum hydrocarbon contaminated soils in Venezuela. Int J Phytoremediation 8: 273-284.
  • Cabalfin EG (2002). Status of radiation treatment of liquid samples in the Philippines. In: The FNCA Workshop on Application of Electron Accelerator, Takasaki, Japan.
  • Chen F, Wang X, Kim HJ (2003). Antioxidant, anticarcinogenic and termiticidal activities of vetiver oil. In: Proceedings of the Third International Vetiver Conference, Guangzhou, China, pp. 546-549.
  • Craigie JS, Morris ER, Rees DA, Thom D (1984). Alginate block structure in Phaeophyceae from Nova Scotia: variation with species, environment and tissue type. Carbohydr Polym 4: 237-252.
  • Danh T (2007). Development of process for purification of α and β-vetivone from vetiver essential oil and investigation of effects of heavy metals on quality and quantity of extracted vetiver oil. PhD, University of New South Wales, Sydney, Australia.
  • Dar TA, Uddin M, Khan MMA, Ali A, Varshney L (2016). Modulation of alkaloid content, growth and productivity of Trigonella foenum-graecum L. using irradiated sodium alginate in combination with soil applied phosphorus. J Appl Res Med Aromat Plants 3: 200-210.
  • Darvill A, Augur C, Bergmann C, Carlson RW, Cheong JJ, Eberhard S, Mohnen D (1992). Oligosaccharins--oligosaccharides that regulate growth, development and defense responses in plants. Glycobiology 2: 181-198.
  • Demole EP, Holzner GW, Youssefi MJ (1995). Malodor formation in alcoholic perfumes containing vetiveryl acetate and vetiver oil. Perfum Flav 20: 35-40.
  • Dwivedi RS, Randhawa NS (1974). Evaluation of rapid test for the hidden hunger of zinc in plants. Plant Soil 40: 445-451.
  • El-Mohdy HA (2012). Radiation-induced degradation of sodium alginate and its plant growth promotion effect. Arab J Chem 10 (Suppl. 1): S431-S438.
  • El-Rehim HAA (2006). Characterization and possible agricultural application of polyacrylamide/sodium alginate crosslinked hydrogels prepared by ionizing radiation. J Appl Polym Sci 101: 3572-3580.
  • Farmer EE, Thomas DM, Michael JS, Clarence AR (1991). Oligosaccharide signaling in plants. J Biol Chem 266: 3140- 3145.
  • Gacesa P (1988). Alginates. Carbohyd Polym 8: 161.
  • Guenther E (1972). The Essential Oils: History - Origin in Plants - Production - Analysis. Vol. 1. Huntington, NY, USA: Robert E. Krieger Publishing Company.
  • Hafiz M, Muhamad O, Muhammad SY, Ahmad MS (2003). Irradiated chitosan as plant promoter. In: Prosiding Simposium Biologic Gunaan Ke-7, Mines Beach Resort & Spa, Seri Kumbangan, Selangor, Malaysia, pp. 3-4.
  • Hecht H, Srebnik S (2016). Structural characterization of sodium alginate and calcium alginate. Biomacromolecules 17: 2160- 2167.
  • Henry RP (1996). Multiple roles of carbonic anhydrase in cellular transport and metabolism. Annu Rev Physiol 58: 523-538.
  • Hien NQ, Nagasawa N, Tham LX, Yoshii F, Dang VH, Mitomo H, Kume T (2000). Growth-promotion of plants with depolymerized alginates by irradiation. Rad Phys Chem 59: 97-101.
  • Hu X, Jiang X, Hwang H, Liu S, Guan H (2004). Promotive effects of alginate-derived oligosaccharide on maize seed germination. J Appl Phycol 16: 73-76.
  • Idrees M, Dar TA, Naeem M., Aftab T, Khan MMA, Ali A, Moinuddin, Varshney L (2014). Effects of gamma-irradiated sodium alginate on lemongrass: field trials monitoring production of essential oil. Ind Crop Prod 63: 269-275.
  • Idrees M, Hassan IU, Naikoo GA, Naeem M, Aftab T, Khan MMA, Varshney L (2016). Functional activities and essential oil production in coriander plants supported with application of irradiated sodium alginate. Int J Appl Environ Sci 11: 467-474.
  • Idrees M, Naeem M, Alam M, Aftab T, Hashmi N, Khan MMA, Varshney L (2011). Utilizing the γ-irradiated sodium alginate as a plant growth promoter for enhancing the growth, physiological activities, and alkaloids production in Catharanthus roseus L. Agr Sci China 10: 1213-1221.
  • Idrees M, Nasir S, Naeem M, Aftab T, Khan MMA, Moinuddin, Varshney L (2012). Gamma irradiated sodium alginate induced modulation of phosphoenolpyruvate carboxylase and production of essential oil and citral content of lemongrass. Ind Crop Prod 40: 62-68.
  • Iwasaki KI, Matsubara Y (2000). Purification of alginate oligosaccharides with root growth-promoting activity toward lettuce. Biosci Biotechnol Biochem 64: 1067-1070.
  • Jaworski EJ (1971) Nitrate reductase assay in intact plant tissues. Biochem Biophys Res Commun 43: 1247-1279.
  • John M, Röhrig H, Schmidt J, Walden R, Schell J (1997). Cell signalling by oligosaccharides. Trends Plant Sci 2: 111-115.
  • Khan NA (1994) Variation in carbonic anhydrase activity and its relationship with dry mass of mustard. Photosynthetica 30: 317-320.
  • Khan NA, Javid S, Samiullah (2004). Physiological role of carbonic anhydrase in CO2 fixation and carbon partitioning. Physiol Mol Biol Plants 10: 153-166.
  • Khan ZH, Khan MMA, Aftab T, Idrees M, Naeem M (2011). Influence of alginate oligosaccharides on growth, yield and alkaloid production of opium poppy (Papaver somniferum L.). Front Agric China 5: 122-127.
  • Kloer DP, Welsch R, Beyer P, Schulz GE (2006). Structure and reaction geometry of geranyl geranyl diphosphate synthase from Sinapis alba. Biochemistry 45: 15197-15204.
  • Kume T, Nagasawa N, Yoshii F (2002). Utilization of carbohydrates by radiation processing. Rad Phys Chem, 63: 625-627.
  • Laule O, Furholz A, Chang HS, Zhu T, Wang X, Heifetz PB, Gruissem W, Lange BM (2003). Crosstalk between cytosolic and plastidial pathways of isoprenoid biosynthesis in Arabidopsis thaliana. P Natl Acad Sci USA 100: 6866-6871.
  • Lavania UC (2003). Vetiver Root – Oil and Its Utilization. Tech. Bull. No. 2003/1. Bangkok, Thailand: PRVN/ORDPB.
  • Lee DW, Choi WS, Byun MW, Park HJ, Yu YM, Lee CM (2003). Effect of γ-irradiation on degradation of alginate. J Agric Food Chem 51: 4819-4823.
  • Luan LQ, Ha VTT, Nagasawa N, Nakanishi TM (2009). A study of degradation mechanism of alginate by gamma-irradiation. Radioisotopes 58: 1-11.
  • Luan LQ, Hien NQ, Nagasawa N, Kume T, Yoshii F, Nakanishi TM (2003). Biological effect of radiation-degraded alginate on flower plants in tissue culture. Appl Biochem Biotechnol 38: 283-288.
  • MacKinney G (1941). Absorption of light by chlorophyll solutions. J Biol Chem 140: 315-322.
  • MacLachlan S, Zalik S (1963). Plastid structure, chlorophyll concentration, frees amino acid composition of chlorophyll mutant of barley. Can J Bot 41: 1053-1062.
  • Mollah MZI, Khan MA, Khan RA (2009). Effect of gamma irradiated sodium alginate on red amaranth (Amaranthus cruentus L.) as growth promoter. Radiat Phys Chem 78: 61-64.
  • Naeem M, Aftab T, Ansari AA, Idrees M, Ali A, Khan MMA, Varshney L (2015). Radiolytically degraded sodium alginate enhances plant growth, physiological activities and alkaloids production in Catharanthus roseus L. J Radiat Res Appl Sci 8: 606-616.
  • Naeem M, Aftab T, Idrees M, Singh M, Ali A, Khan MM, Uddin M, Varshney L (2017). Modulation of physiological activities, active constituents and essential oil production of Mentha arvensis L. by concomitant application of depolymerised carrageenan, triacontanol and 28-homobrassinolide. J Essent Oil Res 29:179-88.
  • Naeem M, Idrees M, Aftab T, Khan MMA, Moinuddin, Varshney L (2011). Irradiated sodium alginate improves plant growth, physiological activities and active constituents in Mentha arvensis L. J Applied Pharm Sci 2: 28-35.
  • Naeem M, Idrees M, Aftab T, Khan MMA, Varshney L (2012). Depolymerised carrageenan enhances physiological activities and menthol production in Mentha arvensis L. Carbohyd Polym 87: 1211-1218.
  • Nagasawa N, Mitomo H, Yoshii F, Kume T (2000). Radiation induced degradation of sodium alginate. Polym Degrad Stab 69: 279- 285.
  • Natsume M, Kamo Y, Hirayama M, Adachi T (1994). Isolation and characterization of alginate-derived oligosaccharides with root growth-promoting activities. Carbohydr Res 258: 187-197.
  • Okabe K, Yang S, Tsuzuki M, Miyachi S (1984). Carbonic anhydrase: its content in spinach leaves and its taxonomic diversity studied with anti-spinach leaf carbonic anhydrase antibody. Plant Sci Lett 33: 145-153.
  • Patel MS, Golakia BA (1988). Effect of water stress on yield attributes and yield of groundnut (Arachis hypogaea L.). Indian J Agr Sci 58: 701-703.
  • Rao KM, Rao KK, Sudhakar P, Rao KC, Subha MCS (2013). Synthesis and characterization of biodegradable poly (vinyl caprolactam) grafted on to sodium alginate and its microgels for controlled release studies of an anticancer drug. J Appl Pharm Sci 3: 61.
  • Sabharwal S (2004). Radiation processing in India: current status and future programme. In: Radiation Processing of Polysaccharides. Vienna, Austria: International Atomic Energy Agency, pp. 9.
  • Sarfaraz A, Naeem M, Nasir S, Idrees M, Aftab T, Hashmi N, Varshney L (2011). An evaluation of the effects of irradiated sodium alginate on the growth, physiological activities and essential oil production of fennel (Foeniculum vulgare Mill.). J Med Plants Res 5: 15-21.
  • Sartori C, Finch DS, Ralph B, Gilding K (1997). Determination of the cation content of alginate thin films by FT-IR spectroscopy. Polymer 38: 43-51.
  • Schalk M, Deguerry F (2013). Cytochrome P450 and Use Thereof for the Enzymatic Oxidation of Terpenes. Patent WO 2013064411 A1.
  • Sellimi S, Younes I, Ayed HB, Maalej H, Montero V, Rinaudo M, Nasri M (2015). Structural, physicochemical and antioxidant properties of sodium alginate isolated from a Tunisian brown seaweed. Int J Biol Macromol 72: 1358-1367.
  • Siddiqui MH, Khan MN, Mohammad F, Khan MMA (2008). Role of nitrogen and gibberellin (GA3 ) in the regulation of enzyme activities and in osmoprotectant accumulation in Brassica juncea L. under salt stress. J Agron Crop Sci 194: 214-224.
  • Swamy KN, Rao SSR (2009). Effect of 24-epibrassinolide on growth, photosynthesis, and essential oil content of Pelargonium graveolens (L.) Herit. Russ J Plant Physiol 56: 616-620.
  • von Wiren N, Gazzarini S, Gojon A, Frommer WB (2000). The molecular physiology of ammonium uptake and retrieval. Curr Opin Plant Biol 3: 254-261.
  • Xin Bin D, Rongxian Z, Wei L, Xiaming X, Schuqing C (2001). Effects of carbonic anhydrase in wheat leaf on photosynthetic function under low CO2 concentration. Sci Agric Sinica 34: 97-100.
Turkish Journal of Biology-Cover
  • ISSN: 1300-0152
  • Yayın Aralığı: Yılda 6 Sayı
  • Yayıncı: TÜBİTAK