Investigation of mode of action of DNA insecticides on the basis of LdMNPV IAP-3 gene

This study investigates the mechanism of action of DNA insecticides, a novel preparation against gypsy moth (Lymantria dispar) based on the DNA fragments of the antiapoptotic gene of its nuclear polyhedrosis virus. In our experiments gypsy moth showed significant susceptibility to applied DNA insecticides on the basis of BIR and RING domains fragments of LdMNPV IAP-3, whereas common fruit fly and tobacco hornworm did not. PCR with the same fragments of BIR and RING domains of the LdMNPV IAP-3 gene as primers revealed parts of the genomes of gypsy moth, common fruit fly, and tobacco hornworm. Part of the gypsy moth genome cloned with the fragments of BIR and RING domains of the LdMNPV IAP-3 gene as primers has an overlap with the corresponding part of the LdMNPV IAP-3 gene and Lymantria dispar IAP-1 mRNA for an inhibitor of apoptosis protein with the high cover of the latter by query, which allows assuming that we cloned a part of a gypsy moth antiapoptosis gene. This finding suggests that DNA insecticides might act through the mechanisms characteristic for blocking of posttranscriptional expression of gypsy moth antiapoptosis genes.

Anahtar Kelimeler:

LdMNPV IAP-3 gene, BIR and RING domains, posttranscriptional silencing, DNA insecticides, crop protection

Investigation of mode of action of DNA insecticides on the basis of LdMNPV IAP-3 gene

Keywords:

LdMNPV IAP-3 gene, BIR and RING domains, posttranscriptional silencing, DNA insecticides, crop protection,

PDF

___

Altschul SF, Madden TL, Schäffer AA, Zhang J, Zhang Z, Miller W, Lipman DJ (1997). Gapped BLAST and PSI-BLAST, a new generation of protein database search programs. Nucleic Acids Res 25: 3389–3402.
Dias N, Stein CA (2002). Antisense oligonucleotides: basic concepts and mechanisms. Mol Cancer Ther 1: 347–355.
Duckett CS, Nava VE, Gedrich RW, Clem RJ, Van Dongen JL, Gilfillan MC, Shiels H, Hardwick JM, Thompson CB (1996). A conserved family of cellular genes related to the baculovirus iap gene and encoding apoptosis inhibitors. EMBO J 11: 2685– 2694.
Fire A, Xu S, Montgomery M, Kostas S, Driver S, Mello C (1996). Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans. Nature 391: 806–811.
Hamshou M, Van Damme EJ, Caccia S, Cappelle K, Vandenborre G, Ghesquière B, Gevaert K, Smagghe G (2013). High entomotoxicity and mechanism of the fungal GalNAc/Gal- specific Rhizoctonia solani lectin in pest insects. J Insect Physiol 59: 295–305.
Herniou EA, Olszewski JA, O’Reilly DR, Cory JS (2004). Ancient coevolution of baculoviruses and their insect hosts. J Virol 78: 3244–3251.
Hughes AL (2002). Evolution of inhibitors of apoptosis in baculoviruses and their insect hosts. Infect Genet Evol 2: 3–10.
Ikeda M, Yamada H, Ito H, Kobayashi M (2011). Baculovirus IAP1 induces caspase-dependent apoptosis in insect cells. J Gen Virol 92: 2654–2663.
Kawai-Toyooka H, Kuramoto C, Orui K, Motoyama K, Kikuchi K, Kanegae T, Wada M (2004). DNA interference: a simple and efficient gene-silencing system for high-throughput functional analysis in the fern Adiantum. Plant Cell Physiol 45: 1648– 1657.
Kuzio J, Pearson MN, Harwood SH, Funk CJ, Evans JT, Slavicek J, Rohrmann GF (1999). Sequence and analysis of the genome of a baculovirus pathogenic for Lymantria dispar. Virology 253: 17–34.
Lin SL, Ying SY (2001). D-RNAi is a novel defense system against cancers and viral infections. Cur Cancer Drug Tar 1: 241–247.
Lu X, Yu Q, Binder GK, Chen Z, Slepushkina T, Rossi J, Dropulic B (2004). Antisense-mediated inhibition of human immunodeficiency virus (HIV) replication by use of an HIV type 1-based vector results in severely attenuated mutants incapable of developing resistance. J Virol 78: 7079–7088.
Oberemok VV (2008a). Method of elimination of phyllophagous insects from order Lepidoptera. Patent of Ukraine No. 36445, Publ. 27/10/2008a, Bull. 20. Kyiv, Ukraine: Ukrainian Patent Office.
Oberemok VV (2008b). Proof of transovarial transmission of Lymantria dispar nucleopolyhedrovirus (Fam. Baculoviridae) with the RAPD-PCR method. Zh Obshch Biol 69: 397–400.
Oberemok VV (2009). Influence of a single chain DNA fragments of virus IAP gene LdnVgp140 on viability of Lymantria dispar caterpillars. Ukr Biochem J 4: 171.
Oberemok VV (2011). DNA markers in investigation of interaction between Lymantria dispar multiple nucleopolyhedrovirus and its host gypsy moth. Simferopol, Ukraine: Taurida National VI Vernadsky University.
Oberemok VV, Simchuk AP, Gninenko YI (2013). DNA insecticides: application of the iap-2 gene single-stranded fragments from three different nucleopolyhedroviruses against second instar gypsy moth larvae. Universal Journal of Applied Science 1: 33–37.
Oberemok VV, Skorokhod OA (2014). Single-stranded DNA fragments of insect-specific nuclear polyhedrosis virus act as selective DNA insecticides for gypsy moth control. Pestic Biochem Physiol 113: 1–7.
Oberemok VV, Zaytsev AS, Simchuk AP (2011). DNA insecticides versus DNA stimulators: every drug is a poison, every poison is a drug. Uchenye Zapiski TNU Ser Biology Chemistry 24: 136–143.
Schliess F, Häussinger D (2005). The cellular hydration state: role in apoptosis and proliferation. Signal Transduction 5: 297–302.
Simchuk AP, Oberemok VV, Ivashov AV (2012). Genetics of interactions between moths, host plants and their enemies in Crimean oak forests and its perspective for their control. In: Cauterruccio L, editor. Moths: Types, Ecological Significance and Control. Hauppauge, NY, USA: Nova Science Publishers, pp. 187–205.
Srinivasula SM, Ashwell JD (2008). IAPs: what’s in a name? Mol Cell 2: 123–135.
Stormo GD (2009). An introduction to sequence similarity (“homology”) searching. Curr Protoc Bioinformatics 27: 3.1.1–3.1.7.
Susurluk H, Toprak U, Gürkan MO (2013). Concentration of sodium dodecyl sulfate used in occlusion body extraction affects Spodoptera littoralis nucleopolyhedrovirus biological activity. Turk J Biol 37: 171–175.
Thompson JD, Higgins DG, Gibson TJ (1994). CLUSTAL W: Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22: 4673–4680.
Toprak U, Coutu C, Baldwin D, Erlandson M, Hegedus D (2014). Development of an improved RNA interference vector system for Agrobacterium-mediated plant transformation. Turk J Biol 38: 40–47.
Wang Y, Zhang H, Li H, Miao X (2011). Second generation sequencing supply an effective way to screen RNAi targets in large scale for potential application in pest insect control. PLoS ONE 6: e18644.
Weiss B, Davidkova G, Zhou LW (1999). Antisense RNA gene therapy for studying and modulating biological processes. Cell Mol Life Sci 55: 334–358.
Zhang Z, Schwartz S, Wagner L, Miller W (2000). A greedy algorithm for aligning DNA sequences. J Comput Biol 7: 203–214.