Ersoy SEVGİ, Gökhan CORAL, A. Murat GİZİR, M. Kemal SANGÜN

141111

Investigation of heavy metal resistance in some bacterial strains isolated from industrial soils

Soil samples were collected from an industrial area in Kazanlı, Mersin, Turkey. Six heavy metals, Cr, Cu, Ni, Co, Cd, and Zn, were tested. ICP-AES analyses were carried out to detect the heavy metal levels of the soil samples. The analysis of test samples revealed high levels of Ni and Cr. A total of 272 Pseudomonas spp. and 161 Bacillus spp. strains were isolated from soils from 4 different sampling locations. The maximum tolerable metal concentrations (MTCs) of Cr, Cu, Ni, Co, Cd, and Zn for each isolate were determined. Resistance to Cr was found in 73.9% of the bacterial strains isolated from soil samples, whereas 26% of the isolates exhibited resistance to Ni, 18.4% to Zn, 11.5% to Cd, 9.2% to Co, and 7.3% to Cu. Observed maximum MTCs were 5 mM for Zn and Cu, 3 mM for Ni, and 2 mM for Cr. Plasmids of sizes 1.8, 2.1, and 28 kb were detected in most Pseudomonas spp. strains with resistance to Cu, Cr, Zn, and Ni.
Anahtar Kelimeler:

Key words: Heavy metal resistance, Pseudomonas, Bacillus, industrial soils

Investigation of heavy metal resistance in some bacterial strains isolated from industrial soils

Soil samples were collected from an industrial area in Kazanlı, Mersin, Turkey. Six heavy metals, Cr, Cu, Ni, Co, Cd, and Zn, were tested. ICP-AES analyses were carried out to detect the heavy metal levels of the soil samples. The analysis of test samples revealed high levels of Ni and Cr. A total of 272 Pseudomonas spp. and 161 Bacillus spp. strains were isolated from soils from 4 different sampling locations. The maximum tolerable metal concentrations (MTCs) of Cr, Cu, Ni, Co, Cd, and Zn for each isolate were determined. Resistance to Cr was found in 73.9% of the bacterial strains isolated from soil samples, whereas 26% of the isolates exhibited resistance to Ni, 18.4% to Zn, 11.5% to Cd, 9.2% to Co, and 7.3% to Cu. Observed maximum MTCs were 5 mM for Zn and Cu, 3 mM for Ni, and 2 mM for Cr. Plasmids of sizes 1.8, 2.1, and 28 kb were detected in most Pseudomonas spp. strains with resistance to Cu, Cr, Zn, and Ni.
Keywords:

Key words: Heavy metal resistance, Pseudomonas, Bacillus, industrial soils,

PDF

___

  • Wiener P, Müller-Graf C, Barcus V. Bacterial evolution in modern times: trends and implications. Integrative Biol 1: 149- 160, 1998. 2. Montuelle B, Latour X, Volat B et al. Toxicity of heavy metals to bacteria in sediments. Bull Environ Contam Toxicol 53: 753- 758, 1994. 3.
  • Atlas RM, Bartha R. Microbial Ecology: Fundamentals and
  • Applications. Addison Wesley Longman, Inc. Menlo Park,
  • California; 1993: pp. 237-238. 4.
  • Nies DH. Microbial heavy metal resistance. Appl Microbiol
  • Biotechnol 51: 730-750, 1999. 5. Said WA, Lewis DL. Quantitative assessment of the effects of metals on microbial degradation of organic chemicals. Appl Environ Microbiol 57: 1498-1503, 1991. 6. Sangün MK, Özdilek HG. Assessment of sea water quality around Sunken MV Ulla ship on İskenderun bay, Hatay-Turkey. Asian J Chem 19: 621-626, 2007.
  • Keilwein G, Stainer RY, Palleroni NJ et al. The aerobic Pseudomonas - a taxonomic study. J Gen Microbiol 42: 159-271, 1966. 8. Bergey’s Manual of Determinative Bacteriology. 9th ed. Hensyl WR. ed. Williams and Wilkins. Baltimore; 1994. 9. Margeay M, Nies D, Schlegel HG et al. Alcaligenes eutrophus CH34 is a facultative chemolitotroph with plasmid bound resistance to heavy metals. J Bacteriol 162: 328-334, 1985.
  • Schmidt T, Schlegel HG. Combined nickel-cobalt-cadmium resistance encoded by the ncc locus of Alcaligenes xylosoxidans 31A. J Bacteriol 176: 7045-7054, 1994.
  • Sambrook J, Russell DW. Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press. Cold Spring Harbor, New York; 2001.
  • Viti C, Giovannetti L. The impact of chromium contamination on soil heterotrophic and photosynthetic microorganisms. Ann Microbiol 51: 201-213, 2001.
  • Malik A, Khan IF, Alem A. Plasmid incidence in bacteria from agricultural and industrial soils. World J Microbiol Biotechnol 18: 827-833, 2002.
  • Malik, A, Jaiswal R. Metal resistance in Pseudomonas strains isolated from soil treated with industrial wastewater. World J Microbiol Biotechnol 16: 177-182, 2000.
  • Konopka A, Zakharova T, Bischoff M et al. Microbial biomass and activity in lead contaminated soil. Appl Environ Microbiol 65: 2256-2259, 1999.
  • Ross DS, Sjorgen RE, Barlett RJ. Behavior of chromium in soils: IV. Toxicity to microorganisms. J Environ Qual 2: 145-168, 1981.
  • Ünaldı MN, Korkmaz H, Arıkan B et al. Plasmid encoded heavy metal resistance in Pseudomonas sp. Bull Environ Contam Toxicol 71: 1145-1150, 2003.
  • Roane TM, Kellogg ST. Characterization of bacterial communities in heavy metal contaminated soils. Can J Microbiol 42: 593-603, 1996.
  • Viti C, Pace A, Giovannetti L. Characterization of Cr(VI) resistant bacteria isolated from chromium contaminated soil by tannery activity. Curr Microbiol 46: 1-5, 2003.
  • Pacheco SV, Miranda R, Cervantes C. Inorganic ion resistance by bacteria isolated from Mexico City freeway. Antonie van Leeuwenhoek 67: 333-337, 1995.
  • Hada HS, Sizemore RK. Incidence of plasmids in marine Vibrio spp. isolated from an oil field in the northwestern Gulf of Mexico. Appl Environ Microbiol 41: 199-202, 1981.
Turkish Journal of Biology-Cover
  • ISSN: 1300-0152
  • Yayın Aralığı: 6
  • Yayıncı: TÜBİTAK
Arşiv
Sayıdaki Diğer Makaleler

Siderophore, hemolytic, protease, and pyrazinamidase activities and antibiotic resistance in motile Aeromonas isolated from fish

Belgin ERDEM, Ergin KARİPTAŞ, Tayfun KAYA

Molecular fingerprinting of some clinically significant Nocardia and related strains by restriction polymorphism ribosomal RNA analyses

Kamil IŞIK, Michael GOODFELLOW

Phenolic compounds and antioxidant and antimicrobial properties of Helichrysum species collected from eastern Anatolia, Turkey

Sevil ALBAYRAK, Ahmet AKSOY, Osman SAĞDIÇ, Ümit BUDAK

The in vitro effect of certain fungicides, insecticides, and biopesticides on mycelial growth in the biocontrol fungus Trichoderma harzianum

Soumik SARKAR, Pradeepa NARAYANAN, Ajay DIVAKARAN

Biological activities of Cu(II) and Hg(II) complexes of a heptadentate Schiff base ligand

Emel ERGENE, Hülya SİVAS, Kadriye BENKLİ

Heavy metal effects on P, Ca, Mg, and total protein contents in embryonic pleopodal eggs and stage-1 juveniles of freshwater crayfish Astacus leptodactylus (Eschscholtz, 1823)

Utku GÜNER

Investigation of heavy metal resistance in some bacterial strains isolated from industrial soils

Ersoy SEVGİ, Gökhan CORAL, A. Murat GİZİR, M. Kemal SANGÜN

Endüstriyel topraklardan izole edilen bazı bakterilerde ağır metal dirençliliğinin araştırılması

Gökhan CORAL, M. Kemal SANGÜN, A. Murat GİZİR, Ersoy SEVGİ

Cadmium-induced antioxidant status and sister-chromatid exchanges in Vicia faba L.

Serpil ÜNYAYAR, Ayşin Güzel DEĞER, Ayla ÇELİK, Fazilet Özlem ÇEKİÇ, Sertan ÇEVİK

Maternal donors of polyploids in Pseudoroegneria (Poaceae: Triticeae) and related genera inferred from chloroplast trnL-F sequences

Yu HAI-QING, Zhang CHUN, Ding CHUN-BANG, Ma XIAO, Zhou YONG-HONG