Soumik SARKAR, Pradeepa NARAYANAN, Ajay DIVAKARAN

The in vitro effect of certain fungicides, insecticides, and biopesticides on mycelial growth in the biocontrol fungus Trichoderma harzianum

The in vitro effect of certain fungicides, insecticides, and biopesticides on mycelial growth of Trichoderma harzianum was evaluated. Seven systemic fungicides, 2 contact fungicides, and 4 biopesticides were tested. A progressive increase in percent inhibition of radial growth in the fungus was observed as the concentrations of the fungicides increased. Among the systemic fungicides, hexaconazole was the most toxic, followed by propiconazole and triflumizole. Toxicity of the contact fungicides was lower than that of the systemic fungicides, among which copper oxychloride and copper hydroxide were highly compatible; no inhibition was observed at lower concentrations. In terms of the insecticides tested, quinalphos and dicofol exhibited toxicity (quinalphos more so), even at the low concentration of 10 ppm. Endosulfan, fenpropathrin, and propargite were less toxic, which indicated the compatibility of these insecticides with T. harzianum. A varying level of inhibition was observed in response to the biopesticides. Although neem-based biopesticides completely inhibited the growth of the biocontrol strain, ovis was compatible with the growth of Trichoderma harzianum. The present results will help delineate the possibility of combining T. harzianum biocontrol agents and agrochemicals for use in an integrated pest management approach.

Anahtar Kelimeler:

Key words: Biological control, Trichoderma harzianum, fungicides, insecticides, biopesticides

The in vitro effect of certain fungicides, insecticides, and biopesticides on mycelial growth in the biocontrol fungus Trichoderma harzianum

Keywords:

Key words: Biological control, Trichoderma harzianum, fungicides, insecticides, biopesticides,

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Monte, E. Understanding Trichoderma: between biotechnology and microbial ecology. Int Microbiol 4: 1-4, 2001.
Kredics L, Antal Z, Manczinger L, Szekeres A, Kevei F, Nagy E. Influence of environmental parameters on Trichoderma strains with biocontrol potential. Food Technol Biotechnol 41: 37-42, 2003.
Locke JC, Marois JJ, Papavizas GC. Biological control of Fusarium wilts of greenhouse-grown Chrysanthemums. Plant Dis 69: 167-169, 1985.
Dhingra OD, Sinclair JB. Basic Plant Pathology Method. Florida. p 132-163. CRC Press, Inc. Boca Raton, 1985
Sundar AR, Das ND, Krishnaveni D. In-vitro antagonism of Trichoderma spp. against two fungal pathogens of Castor. Indian J Plant Protec 23: 152-155, 1995.
Anonymous. ‘ANVIL’ Fungicide (PP 523): Technical Bulletin. Imperial Chemical Industries PLC. Plant Protection Division. Pp.12. Fernhurst, Haslemere, Surrey, England, 1985.
Mizuno M. “Triflumizole (Trifmine(R)): a new broad spectrum fungicide”. Japan Pesticide Information 52:27-30, 1988.
Nene YL, Thapliyal PN. In Fungicides in plant disease control. Pp-691, Oxford & IBH Publishing Company (P) Limited. New Delhi, 1993.
De Cal A, Pascual S, Melgarejo P. In vitro studies on the effects of fungicides on beneficial fungi of peach twig mycoflora. Mycopathologia 126: 15-20, 1994.
Isaiah A, Jain A, Paul MS. Compatibility of Beauveria bassiana with Multineem and Chemical pesticides. Ann Pl Protec Sci 13: 213-269, 2005.
Harlapur SI, Kulkarni MS, Wali MC, et al. Evaluation of plant extract, bio agents and fungicides against Exserohilum turcicum (Pass) Leonard and Suggs. causing turcicum leaf blight of maize. Karnataka J Agric Sci 20: 541-544, 2007.
Biswas K, Chattopadhyay I, Banerjee RK et al. Biological activities and medicinal properties of neem (Azadirachta indica). Current Sci 82: 1336-1345, 2002.