Vu Nguyen DOAN, My Thi Ngoc NGUYEN, Ha Le Bao TRAN

In vitro study on chondrogenic differentiation of human adipose-derived stem cells on treated bovine pericardium

Bovine pericardium has been proposed as an available material for tissue engineering and bioprosthetic reconstruction. In thisstudy, bovine pericardium was fabricated into a scaffold for culturing and chondrogenic differentiation of human adipose-derived stemcells (hADSCs). Bovine pericardium was treated in 10 mM Tris-HCl and 0.15% SDS, followed by crosslinking in 0.1% glutaraldehyde.Treated bovine pericardium (tBP) was characterized as a slight yellowish thin membrane with enhanced tensile strength and strainproperty. The membrane maintained stability under enzymatic conditions for up to 16 days of incubation. The results confirmed tBPas a cell-friendly scaffold for hADSCs due to low cytotoxicity and its ability to support an appropriate attachment and proliferation ofhADSCs. Moreover, there was an accumulation of the extracellular matrix proteoglycan in tBP seeded with hADSCs after 7 and 14days of chondrogenic induction. COMP as a specific marker of chondrogenesis was detected after 7 days, whereas type X-a1 collagen(Col10a1) expression was stable up to day 14. However, minor expression of aggrecan was found. Taken together, these results indicatethat tBP is a potential scaffold for hADSCs for cartilage tissue engineering.

PDF

___

Aguiari P, Fiorese M, Iop L, Gerosa G, Bagno A (2015). Mechanical testing of pericardium for manufacturing prosthetic heart valves. Interactive Cardiovascular and Thoracic Surgery 22 (1): 72-84. doi: 10.1093/icvts/ivv282
Alkan Z, Yigit O, Acioglu E, Bekem A, Azizli E et al. (2011). Tensile characteristics of costal and septal cartilages used as graft materials. Archives of Facial Plastic Surgery 13 (5): 322-326. doi: 10.1001/archfacial.2011.54
Alves da Silva ML, Martins A, Costa-Pinto AR, Correlo VM, Sol P et al. (2011). Chondrogenic differentiation of human bone marrow mesenchymal stem cells in chitosan-based scaffolds using a flow-perfusion bioreactor. Journal of Tissue Engineering and Regenerative Medicine 5 (9): 722-732. doi: 10.1002/term.372
Galeano-Garces C, Camilleri ET, Riester SM, Dudakovic A, Larson DR et al. (2017). Molecular validation of chondrogenic differentiation and hypoxia responsiveness of platelet-lysate expanded adipose tissue-derived human mesenchymal stromal cells. Cartilage 8 (3): 283-299. doi: 10.1177/1947603516659344
Gupta M, Lyon F, Singh AD, Rundle PA, Rennie IG (2006). Bovine pericardium (Tutopatch) wrap for hydroxyapatite implants. Eye 21: 476. doi: 10.1038/sj.eye.6702227
Herman CK, Strauch B (2008). Dorsal augmentation rhinoplasty with irradiated homograft costal cartilage. Seminars in Plastic Surgery 22 (2): 120-123. doi: 10.1055/s-2008-1063571
Ishii CH (2014). Current update in Asian rhinoplasty. Plastic and Reconstructive Surgery Global Open 2 (4): e133-e133. doi: 10.1097/GOX.0000000000000081
Kameli SM, Khorramirouz R, Eftekharzadeh S, Fendereski K, Daryabari SS et al. (2018). Application of tissue‐engineered pericardial patch in rat models of myocardial infarction. Journal of Biomedical Materials Research Part A 106 (10): 2670-2678. doi: 10.1002/jbm.a.36464
Kuroda T, Matsumoto T, Mifune Y, Fukui T, Kubo S et al. (2011). Therapeutic strategy of third-generation autologous chondrocyte implantation for osteoarthritis. Upsala Journal of Medical Sciences 116 (2): 107-114. doi: 10.3109/03009734.2011.552812
Legendre F, Ollitrault D, Gomez-Leduc T, Bouyoucef M, Hervieu M et al. (2017). Enhanced chondrogenesis of bone marrowderived stem cells by using a combinatory cell therapy strategy with BMP-2/TGF-β1, hypoxia, and COL1A1/HtrA1 siRNAs. Scientific Reports 7 (1): 3406-3406. doi: 10.1038/s41598-017- 03579-y
Li R, Guo W, Yang B, Guo L, Sheng L et al. (2011). Human treated dentin matrix as a natural scaffold for complete human dentin tissue regeneration. Biomaterials 32 (20): 4525-4538. doi: 10.1016/j.biomaterials.2011.03.008
Liu ZZ, Wong ML, Griffiths LG (2016). Effect of bovine pericardial extracellular matrix scaffold niche on seeded human mesenchymal stem cell function. Scientific Reports 6: 37089- 37089. doi: 10.1038/srep37089
Madeira C, Santhagunam A, Salgueiro JB, Cabral JM (2015). Advanced cell therapies for articular cartilage regeneration. Trends in Biotechnology 33 (1): 35-42. doi: 10.1016/j. tibtech.2014.11.003
Malone M, Pearlman S (2015). Dorsal augmentation in rhinoplasty: a survey and review. Facial Plastic Surgery 31 (3): 289-294. doi: 10.1055/s-0035-1555616
Marlovits S, Zeller P, Singer P, Resinger C, Vécsei V (2006). Cartilage repair: generations of autologous chondrocyte transplantation. European Journal of Radiology 57 (1): 24-31. doi: 10.1016/j. ejrad.2005.08.009
Miana VV, González EAP (2018). Adipose tissue stem cells in regenerative medicine. Ecancermedicalscience 12: 822-822. doi: 10.3332/ecancer.2018.822
Nguyen AH, Bartlett EL, Kania K, Bae SM (2015). Simple implant augmentation rhinoplasty. Seminars in Plastic Surgery 29 (4): 247-254. doi: 10.1055/s-0035-1566112
Park SW, Kim JH, Choi CY, Jung KH, Song JW (2015). Various applications of deep temporal fascia in rhinoplasty. Yonsei Medical Journal 56 (1): 167-174. doi: 10.3349/ymj.2015.56.1.167
Park YB, Ha CW, Rhim JH, Lee HJ (2018). Stem cell therapy for articular cartilage repair: review of the entity of cell populations used and the result of the clinical application of each entity. American Journal of Sports Medicine 46 (10): 2540-2552. doi: 10.1177/0363546517729152
Pourfarhangi KE, Mashayekhan S, Asl SG, Hajebrahimi Z (2018). Construction of scaffolds composed of acellular cardiac extracellular matrix for myocardial tissue engineering. Biologicals 53 10-18. doi: 10.1016/j.biologicals.2018.03.005
Shiffman MA, Di Giuseppe A (2013). Advanced Aesthetic Rhinoplasty: Art, Science, and New Clinical Techniques. 1st ed. New York, NY, USA: Springer Science & Business Media.
Sung HW, Chang Y, Chiu CT, Chen CN, Liang HC (1999). Crosslinking characteristics and mechanical properties of a bovine pericardium fixed with a naturally occurring crosslinking agent. Journal of Biomedical Materials Research Part A 47: 116-126. doi: 10.1002/(sici)1097-4636(199911)47:2<116::aidjbm2>3.0.co;2-j Tran CT, Huynh DT, Gargiulo C, Tran Le BH, Huynh MH et al. (2013). Adipose tissue can be generated in vitro by using adipocytes from human fat tissue mesenchymal stem cells seeded and cultured on fibrin gel sheet. Cell and Tissue Banking 14 (1): 97-106. doi: 10.1007/s10561-012-9304-6 Tran HLB, Dinh TTH, Nguyen MTN, To QM, Pham ATT (2016). Preparation and characterization of acellular porcinepericardium for cardiovascular surgery. Turkish Journal of Biology 40: 1243-1250. doi: 10.3906/biy-1510-44
Wang CZ, Eswaramoorthy R, Lin TH, Chen CH, Fu YC et al. (2018). Enhancement of chondrogenesis of adipose-derived stem cells in HA-PNIPAAm-CL hydrogel for cartilage regeneration in rabbits. Scientific Reports 8 (1): 10526. doi: 10.1038/s41598- 018-28893-x
Wang Y, Lu C, He C, Chen B, Zheng Y et al. (2018). Construction of a multilayered mesenchymal stem cell sheet with a 3D dynamic culture system. Journal of Visualized Experiments 2018 (140): e58624. doi: 10.3791/58624
Wee JH, Park MH, Oh S, Jin HR (2015). Complications associated with autologous rib cartilage use in rhinoplasty: a metaanalysis. JAMA Facial Plastic Surgery 17 (1): 49-55. doi: 10.1001/jamafacial.2014.914
Wu Q, Dai M, Xu P, Hou M, Teng Y et al. (2015). In vivo effects of human adipose-derived stem cells reseeding on acellular bovine pericardium in nude mice. Experimental Biology and Medicine 241 (1): 31-39. doi: 10.1177/1535370215597193
Wu SC, Chang JK, Wang CK, Wang GJ, Ho ML (2010). Enhancement of chondrogenesis of human adipose derived stem cells in a hyaluronan-enriched microenvironment. Biomaterials 31 (4): 631-640. doi: 10.1016/j.biomaterials.2009.09.089
Xu J, Wang W, Ludeman M, Cheng K, Hayami T et al. (2008). Chondrogenic differentiation of human mesenchymal stem cells in three-dimensional alginate gels. Tissue Engineering Part A 14 (5): 667-680. doi: 10.1089/tea.2007.0272
Yi HG, Choi YJ, Jung JW, Jang J, Song TH et al. (2019). Threedimensional printing of a patient-specific engineered nasal cartilage for augmentative rhinoplasty. Journal of Tissue Engineering 10 2041731418824797. doi: 10.1177/2041731418824797
Zhao L, Detamore MS (2010). Chondrogenic differentiation of stem cells in human umbilical cord stroma with PGA and PLLA scaffolds. Journal of Biomedical Science and Engineering 3 (11): 1041-1049. doi: 10.4236/jbise.2010.311135