dentification of Streptomyces strains isolated from Humulus lupulus rhizosphere and determination of plant growth promotion potential of selected strains

In the present study it was aimed to obtain novel strains of actinomycetes that have the ability to promote plant growth. Forthis, seven soil samples from the rhizosphere of Humulus lupulus (Pazaryeri, Bilecik) were used and potential isolates were obtained.16S rRNA genes of 30 isolates were amplified by PCR and sequenced. Eighteen isolates were found to be closely related to Streptomycetesspp. and they were tested for their proteolytic activity, cellulase activity, phosphate solubility, IAA production, biofilm formation, andgrowth in nitrogen-limited medium. Two isolates, HCI 12 and HCI 36, were selected according to the results of these tests and theireffects on growth of tomato plants (Solanum lycopersicum) were determined. Results indicated that the HCI 12 and HCI 36 strainscaused a significant increase in root and shoot lengths, dry/fresh weights, and vigor index values compared to the control. The changein macromolecular structure including carbohydrates, proteins, and lipids of tomato plants with HCI 12 and HCI 36 inoculation wasdetermined by Fourier transform infrared (FTIR) analysis. The results obtained from FTIR analysis were used in principal componentanalysis to evaluate changes in amide, carbohydrate, and lipid ratios of plant leaves due to microorganism application. Inoculation withthe HCI 12 and HCI 36 strains caused a significant increase in the total carbohydrate and lipid ratio of tomato.

PDF

___

Bortoletto DR, Lima CA, Zezell D, Sato ET, Martinho H (2018). Vibrational spectra calculation of squamous cell carcinoma in the amide band region. Vibrational Spectroscopy 97: 135-139.
Bouyanfif A, Liyanagea S, Hequeta E, Moustaid-Moussaa N, Abidia N (2019). Vibrational spectra calculation of squamous cell carcinoma in the amide band region. Vibrational Spectroscopy 102: 8-15.
Brandenburg K, Funari S, Koch M, Seydel U (1999). Investigation into the acyl chain packing of endotoxins and phospholipids under near physiological conditions by WAXS and FTIR spectroscopy. Journal of Structural Biology 128 (2): 175-186.
Brandenburg K, Seydel U (1996). Fourier transform infrared spectroscopy of cell surface polysaccharides. In: Mantsch HH, Chapman D (editors). Infrared Spectroscopy of Biomolecules. Chichester, UK: Wiley, pp. 159-202.
Cabra Cendales T, Rodríguez González CA, Villota Cuásquer CP, Tapasco Alzate OA, Hernández Rodríguez A (2017). Bacillus effect on the germination and growth of tomato seedlings (Solanum lycopersicum L). Acta Biologica Colombiana 22: 37- 44.
Dean AP, Sigee DC, Estrada B, Pittman JK (2010). Using FTIR spectroscopy for rapid determination of lipid accumulation in response to nitrogen limitation in freshwater microalgae. Bioresource Technology 101 (12): 4499-4507.
de Oliveira Garcia D, Timenetsky J, Baquerizo Martinez M, Francisco W, Sinto SI et al. (2002). Proteases (caseinase and elastase), hemolysins, adhesion and susceptibility to antimicrobials of Stenotrophomonas maltophilia isolates obtained from clinical specimens. Brazilian Journal of Microbiology 33: 157-162.
Dietel K, Beator B, Budiharjo A, Fan B, Borriss R (2013). Bacterial traits involved in colonization of Arabidopsis thalina roots by Bacillus amyloliquefaciens FZB42. Plant Pathology 29 (1): 59- 66.
Fontaine T (1942). Spectrophotometric determination of phosphorus. Industrial Engineering Chemistry Analytical Edition 14 (1): 77-78.
Gong Y, Bai JL, Yang HT, Zhang WD, Xiong YW et al. (2018). Phylogenetic diversity and investigation of plant growthpromoting traits of actinobacteria in coastal salt marsh plant rhizospheres from Jiangsu, China. Systematic and Applied Microbiology 41: 516-527. doi: 10.1016/j.syapm.2018.06.003 Gordon SA, Weber RP (1951). Colorimetric estimation of indoleacetic acid. Plant Physiology 26 (1): 192-195.
Jukes TH, Cantor CR (1969). Evolution of protein molecules. In: Munro HN (editor). Mammalian Protein Metabolism. New York, NY, USA: Academic Press, pp. 21-132.
Kaiser TDL, Pereira EM, dos Santos KRN, Maciel ELN, Schuenck RP et al. (2013). Modification of the Congo red agar method to detect biofilm production by Staphylococcus epidermidis. Diagnostic Microbiology and Infectious Disease 75 (3): 235- 239.
Kazanas N (1968). Proteolytic activity of microorganisms isolated from freshwater fish. Applied Environment Microbiology 16 (1): 128-132.
Kothari R, Prasad R, Kumar V, Singh D (2013). Production of biodiesel from microalgae Chlamydomonas polypyrenoideum grown on dairy industry wastewater. Bioresource Technology 144: 499-503.
Lane DJ (1991). 16S/23S rRNA sequencing. In: Stackebrandt E, Goodfellow M (editors). Nucleic Acid Techniques in Bacterial Systematics. New York, NY, USA: John Wiley and Sons, pp. 115-175.
Li YT, Hwang SG, Huang YM, Huang CH (2018). Effects of Trichoderma asperellum on nutrient uptake and Fusarium wilt of tomato. Crop Protection 110: 275-282.
Ma Q, Wood TK (2009). OmpA influences Escherichia coli biofilm formation by repressing cellulose production through the CpxRA two‐component system. Environmental Microbiology 11 (10): 2735-2746.
Nautiyal CS (1999). An efficient microbiological growth medium for screening phosphate solubilizing microorganisms. FEMS Microbiology Letters 170 (1): 265-270.
Noiret Gros MF, Shinde S, Larsen PE, Zerbs S, Korajczyk PJ et al. (2018). Dynamics of Aspen roots colonization by pseudomanads reveals strain-specific and mycorrhizal-specific patterns of biofilm formation. Frontiers in Microbiology 9: 853.
Rodrı́guez H, Fraga R (1999). Phosphate solubilizing bacteria and their role in plant growth promotion. Biotechnology Advances 17 (4-5): 319-339.
Rodríguez-Navarro DN, Dardanelli MS, Ruíz-Saínz JE (2007). Attachment of bacteria to the roots of higher plants. FEMS Microbiology Letters 272: 127-136.
Sadeghi A, Karimi E, Dahaji PA, Javid MG, Dalvand Y et al. (2012). Plant growth promoting activity of an auxin and siderophore producing isolate of Streptomyces under saline soil conditions. World Journal of Microbiology and Biotechnology 28: 1503- 1509.
Saitou N, Nei M (1987). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Molecular Biology and Evolution 4 (4): 406-425.
Sathya A, Vijayabharathi R, Gopalakrishnan S (2017). Plant growthpromoting actinobacteria: a new strategy for enhancing sustainable production and protection of grain legumes. Biotechnology 7 (2): 102.
Seipke RF, Barke J, Brearley C, Hill L, Yu DW et al. (2011). A single Streptomyces symbiont makes multiple antifungals to support the fungus farming ant Acromyrmex octospinosus. PLoS One 6: e22028.
Sembiring L, Ward AC, Goodfellow M (2000). Selective isolation and characterisation of members of the Streptomyces violaceusniger clade associated with the roots of Paraserianthes falcataria. Antonie van Leeuwenhoek 78 (3-4): 353-366.
Sousa CdS, Soares ACF, Garrido MdS (2008). Characterization of streptomycetes with potential to promote plant growth and biocontrol. Scientia Agricola 65 (1): 50-55.
Tamura K, Stecher G, Peterson D, Filipski A, Kumar S (2013). MEGA6: Molecular Evolutionary Genetics Analysis version 6.0. Molecular Biology and Evolution 30 (12): 2725-2729.
Tokala RK, Strap JL, Jung CM, Crawford DL, Salove MH et al. (2002). Novel plant microbe rhizosphere interaction involving Streptomyces lydicus WYEC108 and the pea plant (Pisum sativum). Applied Environment Microbiology 68: 2161-2171.
Trujillo ME, Alonso Vega P, Rodríguez R, Carro L, Cerda E et al. (2010). The genus Micromonospora is widespread in legume root nodules: the example of Lupinus angustifolius. ISME Journal 4 (10): 1265-1281.
Valli S, Suvathi SS, Aysha O, Nirmala P, Vinoth KP et al. (2012). Antimicrobial potential of Actinomycetes species isolated from marine environment. Asian Pacific Journal of Tropical Biomedicine 2 (6): 469-473.
Vasconcellos RLFd, Silva MCPd, Ribeiro CM, Cardoso EJBN (2010). Isolation and screening for plant growth-promoting (PGP) actinobacteria from Araucaria angustifolia rhizosphere soil. Scientia Agricola 67 (6): 743-746.
Venturi V, Keel C (2016). Signaling in the rhizosphere. Trends in Plant Science 21 (3): 187-198.
Verma VC, Singh SK, Prakash S (2011). Bio-control and plant growth promotion potential of siderophore producing endophytic Streptomyces from Azadirachta indica A. Juss. Journal of Basic Microbiology 51: 550-556. doi: 10.1002/jobm.201000155
Winn M, Casey E, Habimana O, Murphy CD (2014). Characteristics of Streptomyces griseus biofilms in continuous flow tubular reactors. FEMS Microbiology Letters 352: 157-164.