Helianthus annuus L. var.santafe (Ayçiçeği) tohumlarının çimlenmesi ve erken büyüme üzerine tuz ve dışsal putresin'in etkileri

Kontrollu iklim kosullarında, ayçiçegi (Helianthus annuus L. var. Santafe) tohumlarının çimlenmesi ve bazı büyüme parametreleri üzerine tuz (NaCl) ve putresin (Put.)’in ayrı ayrı ve birlikte etkileri incelenmistir. Çalısmada üç farklı tuz konsantrasyonu (50, 100, 200mM) ile üç farklı seviyede (0.01, 1, 2mM) Put. kullanılmıstır. Tek basına tuz, konsantrasyona baglı olarak, tohumların çimlenmesini engellemis yada geciktirmistir. Ayrıca çimlenme döneminde incelenen bazı büyüme parametreleri (radikula uzunlugu, taze ve kuru agırlık) de tuzdan olumsuz yönde etkilenmistir. Tek basına kullanılan Put., konsantrasyona baglı olmaksızın, hem çimlenme yüzdesi hem de incelenen bazı büyüme parametreleri üzerinde etkisiz bulunmustur. Buna karsılık çesitli konsantrasyonlardaki tuz+Put. kombinasyonlarında, Put., tuz stresi altındaki tohumların çimlenmesini arttırmıstır. Özellikle 200mM NaCl+1 ve/veya 2mM Put., kombinasyonlarındaki Put., tuzun çimlenme üzerindeki engelleyici etkisini tamamen ortadan kaldırmıs ve çimlenmeyi, kendi kontrollarına göre, sırasıyla 4.57 ve 3.21 kat arttırmıstır. Yine aynı tuz+Put. kombinasyonlarında radikula uzunlugu, taze ve kuru agırlıkta da önemli artıslar saptanmıstır.

Effects of salt and exogenous putrescine on seed germination and early seedling growth of helianthus annuus.L var.santafe

Effects of salt (NaCl) and putrescine (Put.) and various combinations of these (salt+Put.) on germination and some early growth parameters of sunflower (Helianthus annuus L. var. Santafe) plants, grown under the controlled conditions, were investigated. Salt was used in 3 different concentrations (50, 100, 200mM) and also, Put. was applied in 3 levels (0.01, 1, 2 mM). The salt treatment alone inhibited or delayed the seed germination, depending on concentration. Furthermore, some growth parameters (length of radicle, fresh and dry weights) studied in germination period were adversely affected by salt. In the treatment alone of Put., regardless of the concentration, it was found to be ineffective on germination percentage as well as on some growth parameters under investigation. However, the use of salt+Put. combinations at various concentrations stimulated the germination of seeds under the salt stress. Particularly in 200mM NaCl+1 and/or 2mM Put. combinations, Put. completely overcame the germination-inhibiting effect of salt and so the germinations in these combinations, as compared to their controls, were increased as much as 4.57 and 3.21 fold respectively. It was also shown that the length of radicle, fresh and dry weights in the same salt+Put. combinationswere increased significantly.

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