Ayper Boğa PEKMEZEKMEK, Uğur Seçil BİNOKAY, Kübra AKILLIOĞLU, Yaşar SERTDEMİR

Evaluation of E330-induced developmental toxicity using FETAX

The present study evaluates the teratogenic and toxicological effects of citric acid (E330), a food additive, on Xenopus laevis embryos. The embryos were exposed to a range of E330 concentrations, from stage 8 to 11 of development, for 96 h under static renewal test conditions. The median lethal concentrations, no-observed adverse effect concentration (NOAEC), lowest observed adverse effect concentration (LOAEC), and minimum concentration to inhibit growth (MCIG) values were calculated. The lethal concentration (LC) values LC10, LC20, LC30, LC40, and LC50 determined for E330 exposure were 0.0113, 0.0117, 0.0119, 0.0122, and 0.0124 g/L, respectively. NOAEC and LOAEC values were calculated as 0.001 and 0.01 g/L. Since the effective concentration (EC50) value could not be determined, the E330 teratogenic index (TI), which is LC50/EC50, was not calculated. MCIG was calculated to be 0.010 g/L. The anomaly rate in Xenopus embryos treated with E330 was quite low. Therefore, the endpoint for the Xenopus embryos was whether they were alive or dead at the end of the study. It can be concluded from these observations that using unlimited amounts of E330 may result in preterm birth or abortion in humans, and E330 usage must be reevaluated and, potentially, limited. Moreover, our results confirm that the Frog Embryo Teratogenesis Assay Xenopus (FETAX) can be a useful pretest for integrated biological hazard assessment.

Anahtar Kelimeler:

Key words: Citric acid, E330, Xenopus, FETAX, teratogenicity

Evaluation of E330-induced developmental toxicity using FETAX

Keywords:

Key words: Citric acid, E330, Xenopus, FETAX, teratogenicity,

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Yurttagün M, Ayaz A. Additives: Mistakes and Lines. Hacettepe University Health Science Faculty, Department of Nutrition and Dietetics; Ministry of Health Publication No. 727, Klasmat Printing House, Ankara, Turkey, 2008.
Yurttagün M. Gıda Katkı Maddeleri Kullanımı Hakkında Geniş Kapsamlı Bir Araştırma. Sağlık Vakfı Online, İstanbul. Available from http://www.saglikvakfi.org.tr/html/gkmy.asp?id=58, last accessed 11 April 2013 (in Turkish).
International Programme on Chemical Safety (IPCS INCHEM). Summary of evaluations performed by the joint FAO/WHO expert committee on food additives. Available from http://www. inchem.org/pages/about.html, last accessed 13 December 2010.
Karakaya E. Gıda kontaminantları ve katkı maddeleri. Turkish Society of Toxicology, Ankara. Available from http://www. turktox.org.tr/gida/fr.1-link.html, last accessed 13 December 2010 (in Turkish).
Joint FAO/WHO Expert Committee on Food Additives (JECFA). Evaluation of E330. Available from http://www.inchem.org/ documents/jecfa/jeceval/jec-alt436htm, last accessed 13 December 2010.
US Food and Drug Administration. Inventory of GRAS Notices: Summary of all GRAS Notices. US FDA/CFSAN. 2008-10 Available at http://www.cfsan.fda.gov/~rdb/opa-gras.html. Archived from the original on 11 October 2008.
Berovic M, Legisa M. Citric acid production. Biotechnol Annu Rev 13: 303–343, 2007.
Khadijah AK, Mazharuddin KM. Production of citric acid from citrus fruit wastes by local isolate and MTCC 281 Aspergillus niger strains. IJEST 3: 4849–4856, 2011.