Bioaccumulation of tributyltin and its impact on spermatogenesis in mud crab Scylla serrata (Forskal)

The effects of tributyltin (TBT) on the spermatogenesis of mud crab Scylla serrata were studied. TBT-exposed crabs exhibited a decrease in the gonadosomatic Index and the hepatosomatic Index compared to the crabs in the control group. Bioaccumulation of the TBT levels also varied in different tissues. The treated crabs showed variation in the weight of the testes with respect to TBT concentrations. Histological results clearly indicated that the control crabs showed spermatophores and granular substances were seen filling up the lumen of the seminiferous tubules. The testes of the experimental crabs illustrated that disruption in the seminiferous tubule membrane architecture, arrangement of spermatophores, granular substance, vacuolation, and reduction in the number of epithelial cells of the membrane were apparent in the seminiferous tubules. Biochemical constituents such as protein and glycogen contents also decreased in experimental crabs. Thus, TBT had significantly retarded the reproductive activity and substantially reduced the biochemical constituents ultimately led to impairment of spermatogenesis in the mud crab S. serrata.

Bioaccumulation of tributyltin and its impact on spermatogenesis in mud crab Scylla serrata (Forskal)

The effects of tributyltin (TBT) on the spermatogenesis of mud crab Scylla serrata were studied. TBT-exposed crabs exhibited a decrease in the gonadosomatic Index and the hepatosomatic Index compared to the crabs in the control group. Bioaccumulation of the TBT levels also varied in different tissues. The treated crabs showed variation in the weight of the testes with respect to TBT concentrations. Histological results clearly indicated that the control crabs showed spermatophores and granular substances were seen filling up the lumen of the seminiferous tubules. The testes of the experimental crabs illustrated that disruption in the seminiferous tubule membrane architecture, arrangement of spermatophores, granular substance, vacuolation, and reduction in the number of epithelial cells of the membrane were apparent in the seminiferous tubules. Biochemical constituents such as protein and glycogen contents also decreased in experimental crabs. Thus, TBT had significantly retarded the reproductive activity and substantially reduced the biochemical constituents ultimately led to impairment of spermatogenesis in the mud crab S. serrata.

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  • Mochida K, Ito K, Kono K et al. Molecular and histological evaluation of tributyltin toxicity on spermatogenesis in a marine fish, mimmichong (Fundulus heteroclitus). Aquat Toxicol 83: 73–83, 2007.
  • Antizer-Ladislao B. Environmental levels, toxicity and human exposure to tributyltin (TBT)-contaminated marine environment. A review. Environ Int 34: 292–308, 2008.
  • Wade TL, Garcia-Romero B, Brooks JM. Tributyltin contamination in bivalves from United States coastal estuaries. Environ Sci Tech 22: 1488–1493, 1988.
  • Morcillo Y, Porte C. Interaction of tributyl and triphenyltin with the microsomal monooxygenase system of mollusks and fish from the western Mediterranean. Aquat Toxicol 38: 35–46, 19 Weis JS, Gottilieb J, Kwiatowski J. Tributyltin retards regeneration and produces deformities of limps in the fiddler crab, Uca pugilator. Arch Environ Contam Toxicol 16: 321– 326, 1987
  • Weis JS, Kim K. Tributyltin is a teratogen in producing deformities in limbs of the fiddler crab, Uca pugilartor. Arch Environ Contam Toxicol 17: 583–587, 1988.
  • Duncan J. The toxicology of molluscidies. The organotins. Pharmac Ther 10: 407–429, 1980.
  • Chiliamovitch VP, Kuhn C. Behavioral, hematological studies on acute toxicity of bis (tri-n-butyltin) oxide on Salmo gairdneri Richardson and Tilapia rendalli Boulenger. J Fish Biol 10: 575– 585, 1977.
  • Laughlin RB, French WJ. Comparative study of acute toxicity of a homologous series of trialkyltins to larval shore crabs, Hemigrapsus nudus, and lobster, Homarus americanus. Bull Environ Contam Toxicol 25: 802–809, 1980.
  • Bryan GW. Bioaccumulation of marine pollutants. Phil Trans R Soc Lond B 286: 483–505, 1979.
  • Jacobson T, Sundelin B, Yang G et al. Low dose TBT exposure decreases amphipod immunocompetence and reproductive fitness. Aquat Toxicol 101: 72–77, 2011.
  • Chapman RF. The Insects: Structure and Function. Cambridge University Press. Cambridge; 1972.
  • Sant’Anna BS, Santos DM, Sandron DC et al. Hermit crabs as bioindicators of recent tributyltin (TBT) contamination. Eco Ind 14: 184–188, 2012.
  • Revathi P, Munuswamy N. Effect of TBT on the early embryonic development in the freshwater prawn Macrobrachium rosenbergii (De Man). Chemosphere 79: 922–927, 2010.
  • Küçükoğlu M, Binokay US, Boğa Pekmezekmek A. The effects of zinc chloride during early embryonic development in zebrafish Brachydanio rerio. Turk J Biol 37: 158–164, 2013.
  • Duft M, Schulte-Oehlmann U, Tillman M et al. Biological impact of organotin compounds on mollusks in marine and freshwater ecosystems. Coast Mar Sci 29: 95–110, 2005.
  • Saha S, Ray M, Ray S. Activity of phosphatases in the hemocytes of estuarine edible mud crab, Scylla serrata exposed to arsenic. J Environ Biol 30: 655–658, 2009.
  • Reddy PS, Bhagyalakshmi A. Changes in oxidative metabolism in selected tissues of the crab (Scylla serrata) in response to cadmium toxicity. Ecotoxicol Environ Saf 29: 255–64, 1994.
  • Vijayavel K, Balasubramanian MP. Changes in oxygen consumption and respiratory enzymes as stress indicators in an estuarine edible crab Scylla serrata exposed to naphthalene. Chemosphere 63: 1523–1531, 2006.
  • Singaram G, Harikrishnan T, Chen FY et al. Modulation of immune-associated parameters and antioxidant responses in the crab (Scylla serrata) exposed to mercury. Chemosphere 90: 917–928, 2013.
  • Ruscoe IM, Shelly CC, Williams GR. The combined effects of temperature and salinity on growth and survival of juvenile mud crab (Scylla serrata Forskal). Aquaculture 238: 239–247, 200 Waldock MJ, Waite ME, Miller D et al. Aquatic environmental production: Analytical Methods. The Determination of Total Tin and Organic Compounds in Environmental Samples. Ministry of Agriculture Fisheries and Food Directorate Fisheries. Lowestoft, UK; 1989.
  • Pandey S, Parvezb S, Ansaria RA et al. Effects of exposure to multiple trace metals on biochemical, histological and ultrastructural features of gills of a freshwater fish, Channa punctata Bloch. Chem Biol Interact 174: 183–192, 2008.
  • Bradford MM. A rapid and sensitive method for the qualification of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72: 248–254, 19 Dezwann D, Zandee I. The utilization of glycogen and accumulation of some intermediates during anaerobiosis in Mytilus edulis. Comp Biochem Physiol 43B: 47–52, 1972.
  • Tietz NW. Clinical Guide to Laboratory Tests. WB Saunders. Philadelphia; 1976.
  • Shimizu A, Kimura S. Effect of bis (tributyltin) oxide on gonadal development of a salt water goby, Chasmichtys dolichognatbus: exposure during maturating period. Bull Tokai Reg Fish Res Lab 123: 45–49, 1987 (in Japanese with English abstract).
  • Omura M, Rika O, Kazuhiko K et al. Two-generation reproductive toxicity study of tributyltin chloride in male rats. Toxicol Sci 64: 224–232, 2001.
  • Jegou B, The Sertoli cells in vivo and in vitro. Cell Biol Toxicol 8: 49–54, 1992.
  • Fent K, Hunn J. Organotins in freshwater harbours and rivers: temporal distribution, annual trends and fate. J Environ Toxicol Chem 14: 1123–1132, 1995.
  • Zhang J, Zuo Z, Chen Y et al. Effect of tributyltin on the development of ovary in female cuvier (Sebasticus marmoratus). Aquat Toxicol 83: 174–179, 2007.
  • Lee RF. Metabolism of tributyltin by marine animals and possible linkages to effects. Mar Environ Res 32: 29–35, 1991.
  • Laughlin RB, Guard HE, Coleman WM. Tributyltin in seawater: speciation and octanol-water partition coefficient. Environ Sci Technol 20: 201–204, 1986.
  • Sokal RZ, Madding CE, Swerdloff RS, Lead toxicity and the hypothalamic pituitary testicular axis. Bio Reprod 33: 722–728, 19 Ruby SM, Idler DR, So YP. The effect of sublethal cyanide exposure on plasma vitellogenesis levels in rainbow trout, Salmo gaidneri during early vitellogenesis. Arch Environ Contam Toxicol 15: 603–607, 1986.
  • Ruby SM, Idler DR, So YP. Changes in plasma, liver and ovary vitellogenin in land-locked Atlantic salmon following exposure to sublethal cyanide. Arch Environ Contam Toxicol 16: 507– 510, 1987.
  • Mercier A, Pelletier E, Hamel JF. Metabolism and subtle toxic effects of butyltin compounds in starfish. Aquat Toxicol 28: 259–273, 1994.
  • Kinnberg K, Korsgarrd B, Bjerregaard P. Concentrationdependent effects of nonylphenol on testis structure in adult platyfish, Xiphophorus maculates. Mar Environ Res 50: 169– 173, 2000.
  • Zutshi B, Murthy PS. Ultrastructural changes in testis of gold fish Glossogobius giuris (Ham.) induced by fenthion. Ind J Exp Biol 39: 170–173, 2001.
  • Reddy PS, Bhagyalakshmi A. Elimination of diurnal rhythm of respiration by methyl parathion in crab, Oziotelphusa sensex sensex (Fabricius). Bull Environ Contam Toxicol 53: 192–197, 19 Sujatha CH, Nair SM, Chako J. Tributyltin oxide induced physiological and biochemical changes in trophic estuarine clam. Bull Environ Contam Toxicol 56: 303–310, 1996.
  • Prasantananda B, Panda N, Behera MK. Nickel induced alterations in protein level of some tissues of Heteropneustes fossilis. J Enviorn Biol 21: 117–119, 2000.
  • Nataraj GM, Sundararajulu S, Sivagamasundari S et al. Effect of sublethal concentration of metacystox on the circadian rhythm of bio model oxygen uptake in Channa striatus. Cur Sci 52: 675–678, 1985.
  • Shah DSM, Rajaramani V, Sathyapriya R et al. Effect of tributyltin oxide (TBTO) on glycogen metabolism in estuarine edible blood clam, Anadara rhombea. J Ecotoxicol Environ Monit 8: 133–135, 1998.
  • Kharat PS, Laxmi B, Shejule KB et al. Effect of TBTCL on glycogen profile in freshwater prawn, Macrobrachium kistnensis. World Appl Sci J 7: 1534–1539, 2009.
  • Reddy PS, Devi M, Sorijini R et al. Cadmium and naphthaleneinduced hyperglycemia in the fiddler crab, Uca pugilator: differential modes of action on the neuro endocrine system. Comp Biochem Physiol 107: 57–61, 1994.
  • Reddy PS, Katyayani RV, Fingerman M. Cadmium and naphthalene-induced hyperglycemia in the fiddler crab, Uca pugilator. Bull Environ Contam Toxicol 56: 425–443, 1996.
  • Sarojini R, Indira B, Nagabhushanam R. Effects of the lethal and sub lethal concentrations of two antifouling organometallic compounds CuSO 4 and TBTO on eyestalks of freshwater prawn Cardina weberi. J Freshwater Biol 2: 29–35, 1990.
  • Mane UH, Kulkarni SD. Acute toxicity effect of cadmium on biochemical composition of different body parts of the bivalve mollusc Lamellidens marginalis. Nat Symp Eco Physiol and Conserv Aqua Recourses Abst (8–11 January), Mumbai; 1999.
  • Suresh V. A study on the effects of heavy metals toxicity on a brackish water crab Uca (celuca) Lacteal annulipes (Crane 1975) of Pulicat Lake Tamil Nadu. PhD, University of Madras, Tamil Nadu, India, 2001.
  • Mali RP. Heavy metal induced changes in the glycolatic potential of freshwater female crab, Barytelphusa guerini. J Toxicol Physiol 1: 115–117, 2003.
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