Mehmet Sait EKİNCİ, Emin ÖZKÖSE, İsmail AKYOL

Effects of sequential sub-culturing on the sirvival and enzyme activity of Neocallimastix hurleyensis

Bu çalışmada rumen fungusunun farklı üç enerji kaynağı üzerinde yetiştirilerek uzun süreli adaptasyonu incelenmiştir. Rumen anaerobik fungusu Neocallimastix hurleyensis avisel, ksilan ve glikoz üzerinde 31 alt kültüre kadar yetiştirilmiş ve fungusun hayatta kalabilmesi ve enzimatik aktivitesi gözlemlenmiştir. Anaerobik fungusun bu enerji kaynaklarının her biri üzerinde yaşayabildiği gözlemlenmiştir. N. hurleyensis'm ksilan üzerindeki 31'nci alt kültürden sonraki ksilanaz aktivitesinde ve avisel üzerindeki 31'nci alt kültürden sonraki aviselaz ve karboksimetilselulaz aktivitelerinde önemli miktarda artış olduğu gözlemlenmiştir. N. hurleyensis'm enerji kaynağı olarak glikoz içeren besi ortamı üzerindeki 31'nci alt kültürden sonraki ksilanaz, aviselaz ve karboksimetilselulaz aktivitelerinde önemli düzeyde düşüşler belirlenmiştir.

Neocallimastix hurleyensis' in ardışık olarak alt kültüre alınmasının yaşamına ve enzim aktivitesine etkileri

This study investigated the long-term adaptation of a rumen anaerobic fungus, Neocallimastix hurleyensis, grown on each of 3 different substrates as the sole energy source. The rumen anaerobic fungus was cultivated on Avicel, Xylan and glucose for up to 31 subcultures, and its survival and enzymatic activity were monitored. The fungus was able to survive on each of these substrates. Significant increases in the xylanase, avicelase and carboxymethylcellulase (CMCase) activities of N. hurleyensis were observed after 31 subcultures on Avicel and Xylan. However, there was a significant decrease in these enzyme activities after 31 consecutive subcultures of the N. hurleyensis fungus on the medium containing glucose as the sole energy source.

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