Rnf genes in purple sulfuur bacterium Allochromatiuum vinosum

Allochromatium vinosum fotosentetik, diazotrof, hidrojen sülfit, elementer sulfur ve tiyosülfit gibi kükürtlü bileşikleri yükseltgeyen bir mor kükürt bakterisidir. Bu makalede, bazı organizmalarda azot fiksasyonunda görev alan rnf genlerinin, Allochromatium vinosum'da varlığını gösteriyoruz.

Mor kükürt Bakterisi Allochromatium vinosum' da bulunan Rnf genleri

Allochromatium vinosum is a photosynthetic, diazotrophic purple sulfur bacterium that oxidizes reduced sulfur compounds hydrogen sulfide, elemental sulfur and thiosulfide. In this article, we report the presence of rnf genes in Allochromatium vinosum, some of which have been reported to take part in nitrogen fixation in some species.

PDF

___

Wahlund TM, Madigan MT. Nitrogen fixation by the thermophilic green sulfur bacterium Chlorobium tepidum. J Bacteriol 175: 474-478, 1993.
Hageman RV, Burris RH. Nitrogenase and nitrogenase reductase associate and dissociate with each catalytic cycle. Proc Natl Acad Sci USA 75: 2699-2702, 1978.
Filler WA, Kemp RM, Ng JC et al. The nifH gene product is required for the synthesis or stability of the iron-molybdenum cofactor of nitrogenase from Klebsiella pneumoniae. Eur J Biochem 160: 371-377, 1986.
Shah VK, Chisnell JR, Brill WJ. Acetylene reduction by the iron- molybdenum cofactor from nitrogenase. Biochem Biophys Res Commun 81: 232-23, 1978.
Schmehl M, Jahn A, Meyer zu Vilsendorf A et al. Identification of a new class of nitrogen fixation genes in Rhodobacter capsulatus: a putative membrane complex involved in electron transport to nitrogenase. Mol Gen Genetics 241: 602-615, 1993.
Jouanneau Y, Jeong H-S, Hugo N et al. Overexpression in Escherichia coli of the rnf genes from Rhodobacter capsulatus. Characterization of two membrane-bound iron-sulfur proteins. Eur J Biochem 251: 54-64, 1998.
Jouanneau Y, Meyer C, Asso M et al. Characterization of a nif- regulated flavoprotein (FprA) from Rhodobacter capsulatus. Redox properties and molecular interaction with a [2Fe-2S] ferredoxin. Eur J Biochem 267: 780-787, 2000.
Jeong H-S, Jouanneau Y Enhanced nitrogenase activity in strains of Rhodobacter capsulatus that overexpress the rnf genes. J Bacteriol 182: 1208-1214, 2000.
Curatti L, Brown CS, Ludden PW et al. Genes required for rapid expression of nitrogenase activity in Azotobacter vinelandii. Proc Natl Acad Sci USA 102: 6291-6296, 2005.
Koo MS, Lee JH, Rah SY. A reducing system of the superoxide sensor SoxR in Escherichia coli. EMBO Journal 22: 2614-2622, 2003.
Steuber J. Na(+) translocation by bacterial NADH:quinone oxidoreductases: an extension to the complex-1 family of primary redox pumps. Biochim Biophys Acta, 1505: 45-56, 2001.
Pfennig N, Trüper H. The family Chromatiaceae' in Phototrophic Prokaryotes, ed. RK Clayton WR Systrom, Plenum Pub. Company, 1978.
Sambrook J., Maniatis T, Fritsch EF. Molecular Cloning. A Laboratory Manual. Cold Spring Harbor, NY, Cold Spring Harbor Laboratory Press, 1989
Chen WP, Kuo TT. A simple and rapid method for the preparation of Gram-negative bacterial genomic DNA. Nuc Acids Res 21: 2260, 1993
Higgins D, Thompson J, Gibson T. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nuc Acids Res. 22: 4673-4680, 1994.
Kumar S, Tamura K, Nei M. MEGA3: Integrated software for Molecular Evolutionary Genetics Analysis and sequence alignment. Briefings in Bioinformatics 5: 150-163, 2004.
Saitou N, Nei M. The neighbor-joining method: A new method for reconstructing phylogenetic trees. Mol Biol Evol, 4: 406-425, 1987.