Ali İrfan İLBAŞ, Yasemin EROĞLU, Halil Erhan EROĞLU

Effects of Dosages and Application Periods of EDTA on Morphological and Cytogenetic Characters of Barley (Hordeum vulgare L.) Seedlings

Ethylenediaminetetraacetic acid (EDTA) has many applications in plant research in addition to other applications, such as chelation and medical therapy. The morphological and cytogenetic effects of EDTA on barley seedlings were investigated in the present study. Seeds of Hordeum vulgare L. were treated with different doses of EDTA (30, 60, 90, 120 and 150 mmol/l) and 2 different application periods (20 and 22 h). Except mitotic index, all of the measurements for the investigated characters were carried out on days 7 and 14. EDTA treatments affected germination rates in nearly all treatments. In general, EDTA treatments decreased the root, leaf and coleoptile lengths, but it had the greatest effect on coleoptile length. Increasing the EDTA dosages and treatment periods also decreased the mitotic index compared to the untreated control. The inhibitory effects of EDTA dosages on the mitotic index indicate that EDTA can have genotoxic and mutagenic effects on barley seedlings.

Anahtar Kelimeler:

EDTA, Hordeum vulgare, mitotic index

Effects of Dosages and Application Periods of EDTA on Morphological and Cytogenetic Characters of Barley (Hordeum vulgare L.) Seedlings

Keywords:

EDTA, Hordeum vulgare, mitotic index,

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Yuan HY, Zhang ZL. Effect of free radicals and temperature on sister chromatid exchanges in Hordeum vulgare L. Acta Bot Sin 35: 20-26, 1993.
Blattner FR. Phylogenetic analysis of Hordeum (Poaceae) as inferred by nuclear rDNA ITS sequences. Mol Phylogenet Evol 33: 289-299, 2004.
Fishbein L, Flamm WG, Falk HL. Chemical mutagens’ environmental effects on biological systems. In: An Interdisciplinary Monograph Series, New York, San Fransisco, London; 1970: pp. 364. 4. Redei GP.
Arabidopsis thaliana (L.) Heynh. A review of the
genetics and biology, Biblio Genet 21; 1969: pp. 1-151.
Shen ZG, Si XD, Wang CC et al. Lead phytoextraction from contaminated soil with high-biomass plant species. Journal of Environmental Quality 31: 1893-1900, 2002. 6. King RW, Zeevaart JAD. Enhancement of phloem exudation from cut petioles by chelating agents. Plant Physiol 56: 96-103, 1974.
Urquhart AA, Joy KW. Use of phloem exudate technique in the study of amino acid transport in pea plants. Plant Physiol 68: 750-754, 1981.
Sen SK, Ghunti P. Effect of pre-sowing seed treatments on the germination and seedling growth in papaya. Orissa Journal of Horticulture 4: 38-43, 1976.
Soetisna U, King MW, Roberts EH. Germination test recommendations for estimating the viability of moist or dry seeds of lemon (Citrus limon L. Burm.) and lime (C. aurantifolia (Christm.) Swing.). Seed Sci Technol 13: 87-110, 1985.
Savaflkan Ç, Toker MC. The effects of various doses of gamma irradiation on the seed germination and root tips chromosomes of rye (Secale cereale L.). Turk J Bot 15: 349-359, 1991.
Mazaheri R. Mutation Study on Arabidopsis thaliana with sodium azide and EDTA. Master Thesis, Ankara University Institute of Science, Turkey, 2000.
Soyfer NV. Influence of physiological conditions on DNA repair and mutagenesis in higher plants. Physiol Plant 58: 373-380, 1983.