Özlem BARIŞ, Medine GÜLLÜCE, Fikrettin ŞAHİN, Hakan ÖZER, Hamdullah KILIÇ, Hakan ÖZKAN

Biological Activities of the Essential Oil and Methanol Extract of Achillea biebersteinii Afan. (Asteraceae)

The present work examined the in vitro antimicrobial and antioxidant activities of the essential oil and methanol extract from Achillea biebersteinii Afan. (Asteraceae). The essential oil exhibited antimicrobial activity against 8 bacteria, 14 fungi and the yeast C. albicans, whereas methanolic extract remained inactive. The antioxidative capacity of the samples was evaluated by using DPPH (2,2-diphenyl-1-picrylhydrazyl) and b-carotene/linoleic acid assays. In both assays, the extract showed better antioxidative capacity than the oil. The extract reduced the stable free radical DPPH with lower IC50 value (89.90 µg/ml) than the oil (8900 µg/ml). In the b-carotene/linoleic acid assay, the samples were not effectively able to inhibit the linoleic acid oxidation, exhibiting only 22.7% (the extract) and 16% (the essential oil) inhibitions at 2 mg/ml, far below than that of BHT (97.0%). Total phenolic constituent of the extract was 51 µg/mg (5.1%, w/w) as gallic acid equivalent. GC-MS analysis of the essential oil resulted in the identification of 64 components representing 92.24% of the oil. Piperitone, camphor and 1,8-cineole (eucalyptol) were the main constituents.

Anahtar Kelimeler:

Achillea biebersteinii, Antimicrobial activity, Antioxidant activity, GC-MS analysis, piperitone, camphor, eucalyptol

Biological Activities of the Essential Oil and Methanol Extract of Achillea biebersteinii Afan. (Asteraceae)

Keywords:

Achillea biebersteinii, Antimicrobial activity, Antioxidant activity, GC-MS analysis, piperitone, camphor, eucalyptol,

PDF

___

Adams RP. Identification of Essential Oils components by Gas Chromatography/ Quadrupole Mass Spectroscopy, Allured Publishing Corporation. Illinois, USA, 2001.
Gulluce M, Sokmen M, Daferera D et al. The in vitro antibacterial, antifungal and antioxidant activities of the essential oil and methanol extracts of herbal parts and callus cultures of Satureja hortensis L. Journal of Agricultural and Food Chemistry, 51: 3958-3965, 2003.
Sahin F, Karaman I, Gulluce M et al. Evaluation of antimicrobial activities of Satureja hortensis L. Journal of Ethnopharmacology, 87: 61-65, 2003.
Burits M, Bucar F. Antioxidant activity of Nigella sativa essential oil. Phytotheraphy Research, 14: 323-328, 2000.
Dapkevicius A, Venskutonis R, Van Beek TA et al. Antioxidant activity of extracts obtained by different isolation procedures from some aromatic herbs grown in Lithuania. J of the Science of Food and Agriculture, 77: 140-146, 1998.
Chandler SF, Dodds JH. The effect of phosphate, nitrogen and sucrose on the production of phenolics and solasidine in callus cultures of Solanum lacinitum. Plant Cell Reports, 2: 105, 1983.
Küsmenoğlu S, Bafler KHC, Özek T et al. Constituents of the essential oil of Achillea biebersteinii Afan. J Essent Oil Res 7: 527- 528, 1995.
Jaimand K, Rezaee MB. Comparative study of the essential oils of three Achillea species from Iran. J Essent Oil Res 13: 354-356, 2001.
Rustaiyan A, Komeilizadeh H, Shariatpanahi MS. Comparative study of the essential oils of three Achillea Species from Iran. J Essent Oil Res 10: 207-209, 1998.
Chialva F, Monguzzi F, Manitto P et al. Essential oil constituents of Achillea biebersteinii Afan. J Essent Oil Res 5: 87-88, 1993.
Weyerstahl P, Marschall H, Seelmann I et al. Constituents of the essential oil of Achillea eriophora DC. Flavour Fragrance J 12: 71- 78, 1997.
Chalchat JC, Gorunovic MS, Petrovic SD. Aromatic plants of Yugoslavia. I. chemical composition of the oils of Achillea millefolium L. ssp. pannonica (Scheele) Hayak, A. Crithmifolia W. et K., A. serbica Nym. and A. tanacetifolia All. J Essent Oil Res 11: 306-310, 1999.
Ruberto G, Barrata MT. Antioxidant activity of selected essential oil components in two lipid model systems. Food Chemistry, 69: 167-174, 2000.