VIRGINIA SARROPOULOU, KORTESSA DIMASSI-THERIOU, IOANNIS THERIOS

Effect of sodium nitroprusside on micropropagation and biochemical parameters of CAB-6P and Gisela 6 cherry rootstocks

The effects of sodium nitroprusside (SNP) on micropropagation of CAB-6P and Gisela 6 cherry rootstocks were investigated. Shoot multiplication of CAB-6P was reduced by adding 4.4 $\mu$M 6-benzylaminopurine (BA) and SNP. Gisela 6 microcuttings treated with BA + 30 $\mu$M SNP gave the highest average shoot number (4.15) and multiplication percentage (100%). In CAB-6P, $\alpha$-naphthaleneacetic acid (NAA) + 30 $\mu$M SNP led to the maximum root number (13.79) and fresh weight (0.336 g); average root length (82 mm) and rooting percentage (95.45%) were greatest after adding 40 and 10 $\mu$M SNP + NAA, respectively. In Gisela 6, NAA + 40 $\mu$M SNP resulted in the highest root number (9.75) and fresh weight (0.238 g), while NAA + 30 $\mu$M SNP enabled maximum root length (30.77 mm). Rooting percentage was greatest (84.62%) when Gisela 6 microcuttings were treated only with NAA. Application of SNP alone in CAB-6P reduced leaf chlorophyll, carbohydrate, and pr?line levels. In contrast, 30?50 $\mu$M SNP increased leaf chlorophyll levels of Gisela 6. SNP augmented leaf carbohydrate and proline content, but it diminished root carbohydrate content. In BA + SNP or NAA + SNP treatments, changes in biochemical constituents were dependent on SNP concentrations and genotypes.

Anahtar Kelimeler:

Auxin, carbohydrate level, chlorophyll content, cytokinin, endogenous proline concentration, nitric oxide, plant tissue culture, rhizogenesis, shoot proliferation

Effect of sodium nitroprusside on micropropagation and biochemical parameters of CAB-6P and Gisela 6 cherry rootstocks

Keywords:

Auxin, carbohydrate level, chlorophyll content, cytokinin, endogenous proline concentration, nitric oxide, plant tissue culture, rhizogenesis, shoot proliferation,

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