Fehmi ODABAŞOĞLU, İsmail ÇELİK, Eşref YEĞİN

Effect of experimental diabetes mellitus on plasma lactate deydrogenase and glutamic oxaloacetic transaminase levels in rabbits

Bu çalışmada, diabet 20 erkek, beyaz, Yeni Zelanda ırkı tavşan (Oryctolagus cuniculus huxley) alloxan verilerek (100 mg/kg vucut i.v.) oluşturuldu ve insulin tedavisi yapılmaksızın 16 hafta bekletildi. Plazma enzimlerini ölçümü için kan örnekleri hem diabetik hemde kontrol grublarından alındı. Muameleden sonra laktat dehidrogenaz (LDH) ve glutamik oksalasetik transaminaz (GOT) seviyeleri belirlendi. LDH ve GOT seviyeleri sırasıyla, kontrol grubunda 155,6 ± 41,9 U/L ve 47,0 ± 20,4 U/L iken, diabetli grubunda 286,9 ± 142,9 U/L ve 93,9 ± 62,5 U/L idi. Yapılan istatistiki analiz sonucu, diabetli, plazma LDH değerinin önemli oranda (p < 0,05) yükseldiği ancak GOT yüksekliğinin önemsiz olduğu (p > 0,05) görüldü. Sonuç olarak, LDH diabetes mellitus için bir indikatör olabilirler ancak, diabetes mellitus’un mevcut karakteristik paremetreleri daha iyi birer indikatördürler.

Tavşanlarda deneysel diyabetin plazma laktat dehidrogenaz ve glutamik oksalasetik transaminaz enzim düzeylerine etkisi

Hyperglycemia was induced in 20 male, white, New Zealand rabbits (2-3 kg) (Oryctolagus cuniculus huxley) by alloxan (100 mg/kg body weight, I.V.) and maintained for 16 weeks without insulin treatment. Blood samples were taken from both diabetic and non-diabetic rabbits at the same time for measurement of plasma enzymes. Levels of glutamic oxalacetic transaminase (GOT) and lactate dehydrogenase (LDH) were determined after treatment. The levels of LDH and GOT were 155.6 $pm$ 41.9 U/L and 47.0 $pm$ 20.4 U/L in the control group respectively. These parameters were 286.9 $pm$ 142.9 U/L and 93.9 $pm$62.5 U/L in the diabetic group respectively. It was observed that LDH (p < 0.05) was significantly increased, whereas GOT was not significantly increased (p > 0.05) in the diabetic group. In conclusion, we agree that routine use of LDH measurement to monitor diabetic status may be useful, but the characteristic parameters of diabetes mellitus are better indicators.

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