Melahat BAGIROVA, Rabia ÇAKIR KOÇ, Adil ALLAHVERDİYEV, Melike ERSÖZ

Effect of cell-conditioned media on biomass production of Leishmania parasites

A large amount of parasite biomass production necessitates a higher volume of culture media, and this further increases the overall cost. For this reason, novel and alternative culture supplementary materials have been continuously investigated. The aim of this study was therefore to evaluate the effect of cell-conditioned media, collected as waste material during routine cell culture, on the growth and development of Leishmania parasites in vitro. In this study, for the first time, we show that cell-conditioned medium obtained from MCF-7 culture increased the growth of Leishmania parasites in vitro. These results show that the metabolites that are present in cell-conditioned media may play an important role in in vitro parasite culture. Cell-conditioned media may be used as an economic tool for isolation, cultivation, and obtaining biomass of Leishmania parasites in laboratory conditions.

Anahtar Kelimeler:

Key words: Culture, medium, Leishmania, large-scale, biomass

Effect of cell-conditioned media on biomass production of Leishmania parasites

Keywords:

Key words: Culture, medium, Leishmania, large-scale, biomass,

PDF

___

Datta AK, Datta R, Sen B. Antiparasitic chemotherapy: tinkering with the purine salvage pathway. Adv Exp Med Biol 625: 116-132, 2008.
Wheeler RJ, Gluenz E, Gull K. The cell cycle of Leishmania: morphogenetic events and their implications for parasite biology. Mol Microbiol 79: 647-662, 2011.
Özer N. Emerging vector-borne diseases in a changing environment. Turk J Biol 29: 125-135, 2005.
Allahverdiyev AM, Cakir Koc R, Canim Ates S et al. Leishmania tropica: The effect of darkness and light on biological activities in vitro. Exp Parasitol 128: 318-323, 2011.
Visvesvara GS, Garcia LS. Culture of protozoan parasites. Clin Microbiol Rev 15: 327-328, 2002.
Merlen T, Sereno D, Brajon N et al. Leishmania spp.: completely defined medium without serum and macromolecules (CDM/ LP) for the continuous in vitro cultivation of infective promastigote forms. Am J Trop Med Hyg 60: 41-50, 1999.
Iovannisci DM, Ullman B. High efficiency plating method for Leishmania promastigotes in semi-defined or completely defined medium. J Parasitol 69: 633-636, 1983.
Melo NM, De Azevedo W, Roitman I et al. A new defined medium for cultivating Leishmania promastigotes. Acta Trop 42: 137-141, 1985.
O’Daly JA, Rodriguez MB. Differential growth requirements of several Leishmania spp. in chemically defined culture media. Acta Trop 45: 109-126, 1988.
Jackson JE, Tally JD, Tang DB. An in vitro micromethod for drug sensitivity testing of Leishmania. Am J Trop Med 41: 318- 330, 1989.
Kar K, Mukerji K, Naskar K et al. Leishmania donovani: a chemically defined medium suitable for cultivation and cloning of promastigotes and transformation of amastigotes to promastigotes. J Eukaryot Microbiol 37: 277-279, 1990.
Gupta AK, Saran R. In vitro maintenance of Leishmania donovani promastigotes in a cheap, serum-free, hemin-based, autoclavable culture medium. J Commun Dis 23: 276-277, 1991.
McCarthy-Burke C, Bates PA, Dwyer DM. Leishmania donovani: use of two different, commercially available, chemically defined media for the continuous in vitro cultivation of promastigotes. Exp Parasitol 73: 385-387, 1991.
van der Valk J, Mellor D, Brands R et al. The humane collection of fetal bovine serum and possibilities for serum-free cell and tissue culture. Toxicol in Vitro 18: 1-12, 2004.
Başalp A. Simple production and purification of monoclonal antibodies in serum-free medium. Turk J Biol 24: 189-196, 2000.
Allakhverdiev AM, Akinshina GT. Stimulation of the development of chloroquine-sensitive and -resistant strains of Plasmodium falciparum by cocultivation with Mastomys natalensis cells. Med Parazitol 3: 70-72, 1988 (article in Russian).
Gregoraszczuk E, Ptak A. Involvement of caspase-9 but not caspase-8 in the anti-apoptotic effects of estradiol and 4-OH- Estradiol in MCF-7 human breast cancer cells. Endocr Regul 45: 3-8, 2011.
Chance ML, Peters W, Shchory L. Biochemical taxonomy of Leishmania. I. Observations on DNA. Ann Trop Med Parasitol 68: 307-316, 1974.
Hendricks LD, Wood DE, Hajduk ME. Haemoflagellates: commercially available liquid media for rapid cultivation. Parasitology 76: 309-316, 1978.
Childs GE, Foster KA, McRoberts MJ. Insect cell culture media for cultivation of New World Leishmania. Int J Parasitol 8: 255- 258, 1978.
Childs GE, McRoberts MJ, Moussa MA. Systems for the in vitro large-scale propagation of New World Leishmania. Ann Trop Med Parasitol 73: 395-396, 1979.
Elgazwy ASH, Yasinzai MM. Sensitivity of kinetoplastids to aminoglycoside: correlation with the 3’ region of the small subunit rRNA gene. Turk J Biol 32: 167-174, 2008.
Hodgson J. To treat or not to treat: that is the question for serum. Nat Biotechnol 13: 333-334, 1995.
Jochems CE, van der Valk JB, Stafleu FR et al. The use of fetal bovine serum: ethical or scientific problem? Altern Lab Anim 30: 219-227, 2002.
Allahverdiyev A, Bağırova M, Çakır Koç R et al. Approaches and problems in vaccine development against leishmaniasis. T Parazitol Derg 34:122-130, 2010 (article in Turkish with English abstract).
Halama A, Moller G, Adamski J. Metabolic signatures in apoptotic human cancer cell lines. OMICS 15: 1-11, 2011.
Mazier D, Druilhe P, Guguen-Guillouzo C et al. Hepatocytes as feeder-layers for in vitro cultivation of Plasmodium falciparum blood-stages. Trans R Soc Trop Med Hyg 78: 330-334, 1984.