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Decolorization of orange II dyey with the crude culture of white rot fungus, Coriolus versicolor

Bu çalışmada beyaz çürükçül fungus olan Coriolus versicolor'un kültür filtratının Orange II boyasının renk giderimi üzerine etkisi araştırılmıştır. Renk giderim hızı ve genişliğinin fungus ham kültür süzüntüsünün yaşına (üreme safhasına) bağlı olarak değiştiği saptanmıştır. 12 gün üretilmiş Funalia trogii, Pleurotus sajor-caju and Phanerochaete chrysosporium ME446'nın ham kültür filtratlarının da renk giderim yetenekleri araştırılmıştır. Phanerochaete chrysosporium ME446 kullanılan 4 boyanın hiçbirinin rengini giderememiştir. Enzim inhibitörü olan siyanid ve azid, kültür filtratının boya rengini giderim yeteneğini inhibe etmişlerdir. Kültür filtratının ısı ile muamelesi de renk giderim aktivitesini sonlandırmıştır Bu çalışmada elde edilen renk giderim aktivitesi mangan bağımsız bir aktivitedir ve veratril alkolün etkisi yoktur. Sonuçlar Orange II nin renginin gideriminde hem H O bağımlı ve hem de H O bağımsız enzimlerin rol oynayabileceğini göstermiştir. Ayrıca, bu çalışma kültür süzüntüsünün yaşı ile renk giderim aktivitesinin ilişkisini ortaya koyan ilk çalışmadır.

Coriolus versicolor ham kültür filtratı kullanarak orange II boyasının renginin giderimi

Decolorization of Orange II dye was. demonstrated with crude culture filtrate of white rot fungus, Coriolus versicolor. It was determined that the rate and extent of decolorization were depend¬ent on the age (growth phase) of the crude culture filtrate (CCF). Dye decolorization abilities of the CCF of Funalia trogii, Pleurotus sajor-caju and Phanerochaete chrysosporium ME446, after incubation for 12 days, were also studied. Phanerochaete chrysosporium ME446 was observed to be unable to decolorize any of 4 dyes used. Cyanide and azide, which are known inhibitors of enzyme, inhibited dye decol-orization ability of the CCF. Heat treatment terminated decolorization activity of CCF. The decolorization activity determined in this study was manganase independent activity and veratryl alcohol had no influence on it. Results showed that both H202 dependent and independent enzymes could play a role in the reduction of the color of Orange 11 dye. Moreover, this is the first study which correlates the age of culture filtrate with decolorization activity.

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1.Cripps C., Bumpus A. J., Aust D. S., Biodegradation of azo and heterocyclic dyes by Phanerochaete chrysospori-um. Appl. Environ. Microbiol. 56: 1114-118, 1990.
2.Ollikka P., Alhonmaki K., Leppanen V-M., Glumoff T., Raijola T., Suominen I., Decolorization of azo, triphenylmethane, heterocyclic, and polymeric dyes by lignin peroxidase yzoenzymes from Phanerochaete chrysosporium.Appl. Environ. Microbiol. 59: 4010-4016, 1993.
3.Meyer U., Biodegradation of synthetic organic colorants. FEMS Symp. 12: 371-385, 1981.
4.Bumpus A. J., Brock J. B., Biodegradation of crystal violet by white rot fungus Phanerochaete chrysosporium.Appl. Environ. Microbiol. 58: 3598-3604, 1988.
5.Paszczynski A., Pasti-Grigsby B. M., Goszczynski S., Crawford L. R., Crawford L. D., Mineralization sulfonated azodyes and sulfanalic acid by Phanerochaete chrysosporiumand Streptomyces chromofuscus. Appl. environ.Microbiol. 58: 3598-3604, 1992
6.Yadav S. J., Reddy A. C., Mineralization of 2, 4-dichlorophenoxyacetic acid (2, 4- D and mixtures of 2,4-D and 2,4, 5-trichlorophenoxyacetic acid by Phanerochaete chrysosporium. Appl. Environ. Microbiol. 59: 2904-2908,1993.
7.Yeşilada Ö., Fışkın, K.., Decolorization of alcholic waste water by white rot fungi Coriolus versicolor, Funalia trogiiand Phanerochaete chrysosporium ME446. Tr. J. of Biology. 19: 191-200, 1995.
8.Yeşilada Ö., Fışkın, K.., Yeşilada E., The use of white rot fungus Funalia trogii(Malatya) for the decolourizationand phenol removal from olive mill wastewater. Environ. Technol. 16 : 95-100, 1995.
9.Paszczynski A., Crawford L. R., Degardation of azo compounds by ligninase from Phanerochaete chrysosporium:Involvement of veratryl alcohol. Biochemical and Biophysical Research Communications. 136: 220-227, 1992.
10. Paszczynski A., Pasti-Grigsby B. M., Foszczynski S., Crawford L. D., Crawford L. R., New approach to improvedegradation of recalcitrant azo dyes by Streptomyces spp. and Phanerochaete chrysosporium. Enzyme Microb.Technol. 13: 378-384, 1991.
11. Pasti-Grigsby B. M., Paszczynski A., Goszczynski S., Crawford L. D., Crawford L. R., Influence of aromatic sub-stution patterns on azo dye degradability by Streptomyces spp. and Phanerochaete chrysosporium. Appl. Environ.Microbiol. 58: 3605-3613, 1991.
12. Spadaro T. J., Gold H. M., Renganathan V., Degardation of azo dyes by the lignindegrading fungus Phanerochaetechrysosporium. Appl. Environ. Microbiol. 58: 2397-2401, 1992.
13.Greene V. R., Gould M. J., H2O2-dependent decolorization of Poly R-481 by particulate fractions fromPhanerochaete chrysosporium. Biochemical and Biophysical Research Communications. 136: 220-227, 1986.
14.Vyas M., R. B., Molitoris P. H., Involvement of an extracellular H202-dependent ligninolytic activity of the whiterot fungus Pleurotus ostreatusin the decolorization of Remazol Brilliant Blue R. Appl. Environ. microbiol. 61: 11:3919-3927, 1995.
15.Işıloğlu M., Watling R., Macromycetes of Mediteranean Turkey, Edinburgh Journal of Botany, 49: 99-121, 1992.
16.Işıloğlu M., Önder N., Malatya yöresinin makrofungusları (Tr. J. of Biology, in press).
17.Ander P., Eriksson EK., The importance of phenol oxidase activity in lignin degradation by the white rot fungusSporotrichum pulverulentum. Archives of Microbiology. 1090: 1-8, 1976.
18.Arora D. S., Sandhu D. K.,, Laccase production and wood degradation by a white rot fungus Daedalea flavida.Enzyme Microb. Technol. 7: 405-408, 1985.
19.Yeşilada Ö., Fışkın K.,, The use of white rot fungi for evaluation and degradation of vinasse. Tr. J. of Biology, 19:181-189, 1995.
20.Kim J. S., Ishıkawa K., Hirai M., Shoda M., Characteristics of a newly ısolated fungus, Geotrichum candidum Dec1, which decolorizes various dyes. Journal of Fermentation and Bioengineering. 79: 601-607, 1995.
21.Chivukula M., Renganathan V., Phenolic azo dye oxidation by laccase from Pyricularia oryzae. Appl. Environ.Microbiol. 61: 4374-4377, 1995.
22.Yeşilada Ö., Decolourization of crystal violet by fungi. World Journal of Microbiology & Biotechnology. 11: 601-602, 1995.