Decolorization of Orange II dye was demonstrated with crude culture filtrate of white rot fungus, Coriolus versicolor. It was determined that the rate and extent of decolorization were depend-ent on the age (growth phase) of the crude culture filtrate (CCF). Dye decolorization abilities of the CCF of Funalia trogii, Pleurotus sajor-caju and Phanerochaete chrysosporium ME446, after incubation for 12 days, were also studied. Phanerochaete chrysosporium ME446 was observed to be unable to decolorize any of 4 dyes used. Cyanide and azide, which are known inhibitors of enzyme, inhibited dye decolorization ability of the CCF. Heat treatment terminated decolorization activity of CCF. The decol-orization activity determined in this study was manganase independent activity and veratryl alcohol had no influence on it. Results showed that both H 2 O 2 dependent and independent enzymes could play a role in the reduction of the color of Orange II dye. Moreover, this is the first study which correlates the age of culture filtrate with decolorization activity.

Decolorization of Orange II dye was demonstrated with crude culture filtrate of white rot fungus, Coriolus versicolor. It was determined that the rate and extent of decolorization were depend-ent on the age (growth phase) of the crude culture filtrate (CCF). Dye decolorization abilities of the CCF of Funalia trogii, Pleurotus sajor-caju and Phanerochaete chrysosporium ME446, after incubation for 12 days, were also studied. Phanerochaete chrysosporium ME446 was observed to be unable to decolorize any of 4 dyes used. Cyanide and azide, which are known inhibitors of enzyme, inhibited dye decolorization ability of the CCF. Heat treatment terminated decolorization activity of CCF. The decol-orization activity determined in this study was manganase independent activity and veratryl alcohol had no influence on it. Results showed that both H 2 O 2 dependent and independent enzymes could play a role in the reduction of the color of Orange II dye. Moreover, this is the first study which correlates the age of culture filtrate with decolorization activity.