Ali Asghar MOSHTAGHIE, Pedram MALEKPOURI, Elham MOSHTAGHIE, Farhang RAHNAMAIE

Cobalt induces alterations in serum parameters associated with bone metabolism in male adult rat

The intention of this study was to investigate the toxic effect of cobalt on the bone metabolism of rats, including calcium, inorganic phosphorus, and bone-specific alkaline phosphatase of serum during short- and long-term experiments. Rats were injected intraperitoneally (IP) with either 16 mg kg-1 BW of cobalt for 5 and 10 days or 6 mg kg-1 BW for 20 and 40 days. It was found that IP administrations of cobalt could increase all serum parameters associated with bone metabolic processes. The observed effect was greatest for 16 mg kg-1 BW when used for 10 continuous days. By using gel filtration chromatography, we found that most of the activity of total serum alkaline phosphatase was related to low molecular weight isoenzymes, which can be used as a biomarker for bone metabolic disturbances. These results suggest that cobalt can induce bone resorption in adult rats and, therefore, behave as an osteoporotic agent.

Anahtar Kelimeler:

Alkaline phosphatase, bone metabolism, bone resorption, cobalt, trace elements

Cobalt induces alterations in serum parameters associated with bone metabolism in male adult rat

Keywords:

Alkaline phosphatase, bone metabolism, bone resorption, cobalt, trace elements,

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Andrews RE, Shah KM, Wilkinson JM, Gartland A (2011). Effects of cobalt and chromium ions at clinically equivalent concentrations after metal-on-metal hip replacement on human osteoblasts and osteoclasts: implications for skeletal health. Bone 49: 717–723.
Anissian L, Stark A, Dahlstrand H, Granberg B, Good V, Bucht E (2002). Cobalt ions influence proliferation and function of human osteoblast-like cells. Acta Orthop Scand 73: 369–374.
ATSDR (2004). Toxicological profile for cobalt. Atlanta, GA, USA: United States Department of Health and Human Services, Public Health Service, Agency for Toxic Substances and Disease Registry.
Baker SB, Worthley LI (2002). The essentials of calcium, magnesium and phosphate metabolism: part II. Disorders. Crit Care Resusc 4: 307–315.
Barceloux DG (1999). Cobalt. Clin Toxicol 37: 201–216.
Bessey OA, Lowry OH, Brock MJ (1946). A method for the rapid determination of alkaline phosphatase with five cubic millimeters of serum. J Biol Chem 164: 321–329.
Boyce BF, Fell GS, Elder HY, Junor BJ, Elliot HL, Beastall G, Fogelman I, Boyle IT (1982). Hypercalcaemic osteomalacia due to aluminium toxicity. Lancet 6: 1009–1013.
Campbell JR, Estey MP (2013). Metal release from hip prostheses: cobalt and chromium toxicity and the role of the clinical laboratory. Clin Chem Lab Med 51: 213–220.
Ciancaglini P, Pizauro JM, Leone FA (1995). Mechanism of action of cobalt ions on rat osseous plate alkaline phosphatase. J Inorg Biochem 60: 155–162.
Conti MI, Bozzini C, Facorro GB, Lee CM, Mandalunis PM, Piehl LL, Piñeiro AE, Terrizzi AR, Martínez MP (2012). Lead bone toxicity in growing rats exposed to chronic intermittent hypoxia. Bull Environ Contam Toxicol 89: 693–698.
Domingo J (1989). Cobalt in the environment and its toxicological implications. Rev Environ Contam Toxicol 108: 105–132.
Fiske CH, Subbarow Y (1925). The colorimetric determination of phosphorus. J Biol Chem 66: 375–400.
Goc Rasgele P, Kekecoglu M, Gokalp Muranli FD (2013). Induction of micronuclei in mice bone marrow cells by cobalt and copper chlorides. Arch Environ Prot 39: 75–82.
Gross M, Kumar R (1990). Physiology and biochemistry of vitamin D-dependent calcium binding proteins. Am J Physiol 259: F195–209.
Hamilton EI (1994). The geobiochemistry of cobalt. Sci Total Environ 150: 7–39.
Jefferson JA, Escudero E, Hurtado ME, Pando J, Tapia R, Swenson ER, Prchal J, Schreiner GF, Schoene RB, Hurtado A et al. (2002). Excessive erythrocytosis, chronic mountain sickness, and serum cobalt levels. Lancet 359: 407–408.
Jelkmann W (2012). The disparate roles of cobalt in erythropoiesis, and doping relevance. Open Journal of Hematology 3–6.
Kechrid ZF, Dahdouh RM, Djabar N, Bouzerna N (2006). Combined effect of water contamination with cobalt and nickel on metabolism of albino (Wistar) rats. Iran J Environ Health Sci Eng 3: 65–69.
Kozuka H (1995). Interactive exhibition of heavy metal toxicity in bone metabolism. From the viewpoint of deductive toxicology. J Pharm Soc Jpn (Yakugaku Zasshi). 115: 157–169 (article in Japanese).
Krishnan SS, Lui SM, Jervis RE, Harrison JE (1990). Studies of cadmium uptake in bone and its environmental distribution. Biol Trace Elem Res 26–27: 257–261.
Langton DJ, Jameson SS, Joyce TJ, Webb J, Nargol AV (2008). The effect of component size and orientation on the concentrations of metal ions after resurfacing arthroplasty of the hip. J Bone Joint Surg Br 90: 1143–1151.
Larese FL, Maina G, Adami G, Venifer M, Coceani N, Bussani R, Massiccio M, Barbieri P, Spinelli P (2004). In vitro percutaneous absorption of cobalt. Int Arch Environ Health 77: 85–95.
Lasfargues G, Lison D, Maldague P, Lauwerys R (1992). Comparative study of the acute lung toxicity of pure cobalt powder and cobalt-tungsten carbide mixture in rat. Toxicol Appl Pharmacol 112: 41–50.
Linna A, Oksa P, Groundstroem K, Halkosaari M, Palmroos P, Huikko S, Uitti J (2004). Exposure to cobalt in the production of cobalt and cobalt compounds and its effect on the heart. Occup Environ Med 61: 877–885.
Lison D, De Boeck M, Verougstraete V, Kirsch-Volders M (2001). Update on the genotoxicity and carcinogenicity of cobalt compounds. Occup Environ Med 58: 619–625.
Lison D (2007). Cobalt. In: Nordberg GF, Fowler BA, Nordberg M, Friberg L, editors. Handbook of the Toxicology of Metals. 3rd ed. Amsterdam, the Netherlands: Elsevier, pp. 511–528.
Malekpouri P, Moshtaghie AA, Kazemian M, Soltani M (2011). Protective effect of zinc on related parameters to bone metabolism in common carp fish (Cyprinus carpio L.) intoxified with cadmium. Fish Physiol Biochem 37: 187–196.
Mirhashemi SM, Moshtaghie AA, Ani M, Aarabi MH (2009). Changes in serum, liver, and brain high and low molecular weight alkaline phosphatase following manganese toxicity in rats. Toxicol Environ Chem 91: 349–355.
Moorehead WR, Briggs HC (1974). 2-Amino-2-methyl-1-propanol as the alkalizing agent it an improved continuous flow cresolphthalein complexone procedure for calcium in serum. Clin Chem 20: 1458–1460.
Moshtaghie AA, Ani M, Soltani M (1995). High molecular weight alkaline phosphatase as a tumor marker for liver cancer; a comparative study with bone and intestine cancers. Clin Chem Enzymol Commun 7: 9–16.
Moshtaghie AA, Ani M, Mirhashemi SM (2006a). Comparative effects of aluminium on serum, liver and brain high and low molecular weight alkaline phosphatase in rats. J Med Sci 6: 848–852.
Moshtaghie AA, Ani M, Mirhashemi SM (2006b). Comparative effects of lead on serum, liver and brain high molecular weight alkaline phosphatase in rats. Pak J Biol Sci 9: 2278–2282.
Patntirapong S, Habibovic P, Hauschka PV (2009). Effects of soluble cobalt and cobalt incorporated into calcium phosphate layers on osteoclast differentiation and activation. Biomaterials 30: 548–555.
Pedigo NG, George WJ, Anderson MB (1988). Effects of acute and chronic exposure to cobalt on male reproduction in mice. Reprod Toxicol 2: 45–53.
Qureshi AA, Virdi AS, Didonna ML, Jacobs JJ, Masuda K, Paprosky WP, Thonar EJ, Sumner DR (2002). Implant design affects markers of bone resorption and formation in total hip replacement. J Bone Miner Res 17: 800–807.
Romslo I, Sagen N, Haram K (1975). Serum alkaline phosphatase in pregnancy. I. A comparative study of total, L-phenylanine- sensitive and heat-stable alkaline phosphatase at 56 degrees C and 65 degrees C in normal pregnancy. Acta Obstet Gynecol Scand 54:437–42.
Sansone V, Pagani D, Melato M (2013). The effects on bone cells of metal ions released from orthopaedic implants. A review. Clin Cases Miner Bone Metab 10: 34–40.
Sigma-Aldrich (2006). Material Safety Data Sheet. www.sigma- aldrich.com (29 August 2006).
Simonsen LO, Harbak H, Bennekou P (2012). Cobalt metabolism and toxicology—a brief update. Sci Total Environ. 432: 210– 215.
Sinko PJ (2006). Martin’s Physical Pharmacy and Pharmaceutical Sciences: Physical Chemical and Biopharmaceutical Principles in the Pharmaceutical Sciences. Baltimore, MD, USA: Lippincott Williams and Wilkins.
Stepensky D, Kleinberg L, Hoffman A (2003). Bone as an effect compartment: models for uptake and release of drugs. Clin Pharmacokinet 42: 863–881.
Sun ZL, Wataha JC, Hanks CT (1997). Effects of metal ions on osteoblast-like cell metabolism and differentiation. J Biomed Mater Res 34: 29–37.
Teitelbaum SL (2000). Bone resorption by osteoclasts. Science 289: 1504–1508.
Yamaguchi M, Inamoto K, Suketa Y (1986). Effect of essential trace metals on bone metabolism in weanling rats: comparison with zinc and other metals’ actions. Res Exp Med (Berl) 186: 337– 342.
Zijlstra WP, Bulstra SK, van Raay JJ, van Leeuwen BM, Kuijer R (2012). Cobalt and chromium ions reduce human osteoblast- like cell activity in vitro, reduce the OPG to RANKL ratio, and induce oxidative stress. J Orthop Res 30: 740–747.