Krystyna GÓRECKA, Waldemar KISZCZAK, Dorota KRZYZANOWSKA, Urszula KOWALSKA, Agata KAPUSCINSKA

Effect of polyamines on in vitro anther cultures of carrot (Daucus carota L.).

This study examines the influence of the polyamines putrescine and spermidine on the efficiency of androgenesis in anther cultures of 2 carrot cultivars, Kazan F1 and Narbonne F1, and the effect of putrescine on the process of plant regeneration from androgenetic embryos. In the Kazan F1 variety, an increase in the number of obtained embryos was achieved using each of the 2 polyamines separately and in combination. In contrast, no beneficial effects of polyamines on the efficiency of androgenesis were observed in the Narbonne F1 variety. Putrescine added to the regeneration medium increased the number of obtained plants. For embryos obtained on induction medium without putrescine, the best concentration was 160 mg putrescine in 1 L of regeneration medium, and for those obtained on the induction medium with putrescine the best concentration was 0.5 mg putrescine per 1 L. All of the resulting plants, both in the experimental and control combinations, had a doubled set of chromosomes. The vast majority of them were homozygous for both isoenzymes, glucose-6-phosphate isomerase and aspartate aminotransferase, and the distribution of homo- and heterozygous received combinations with the polyamines and in the control was very similar.

Anahtar Kelimeler:

Carrot, androgenesis, putrescine, spermidine, regeneration

Effect of polyamines on in vitro anther cultures of carrot (Daucus carota L.).

Keywords:

Carrot, androgenesis, putrescine, spermidine, regeneration,

PDF

___

Bajaj YPS (1990). In vitro production of haploids and their use in cell genetics and plant breeding. In: Bajaj YPS, editor. Haploids in Crop Improvement. Berlin: Springer-Verlag, pp. 3–30.
Bhojwani SS, Dantu PK (2010). Haploid plants. In: Davey MR, Anthony P, editors. Plant Cell Culture: Essential Methods. Chichester, UK: Wiley-Blackwell, pp. 61–76.
Chiancone B, Tassoni A, Bagni N, Germanà MA (2006). Effect of polyamines on in vitro anther culture of Citrus clementina Hort. ex Tan. Plant Cell Tiss Org 87: 145–153.
Cho UH, Kasha KJ (1992). Relationship of senescence to androgenesis in barley (Hordeum vulgare L. cv. Klages). J Plant Physiol 139: 299–302
Cohen SS (1998). A Guide to the Polyamine Metabolism. New York, NY, USA: Oxford University Press.
Datta SK (2005). Androgenic haploids: factors controlling development and its application in crop improvement. Curr Sci India 89: 1870–1878.
Dewi IS, Purwoko BS, Aswidinnoor H, Somantri IH (2004). Rice anther culture in media containing polyamines. J Biotek Pertan 9: 4–19.
Dewi IS, Purwoko BS (2008). Role of polyamines in inhibition of ethylene biosynthesis and their effects on rice anther culture development. Indones J Agric Sci 9: 60–67.
Dewi IS, Purwoko BS, Aswidinnoor H, Somantri IH, Chozin MA (2006). Plant regeneration from anther culture of several accessions of Indica rice tolerant to aluminum. J AgroBiogen 2: 30–35 (article in Indonesian with abstract in English).
Forodi BR, Hassandokht M, Kashi A, Sepahvand N (2009). Influence of spermidine on haploid plant production in Iranian onion (Allium cepa L.) populations through in vitro culture. Hort Environ Biotech 50: 461–466.
Galston AW, Kaur-Sawhney R (1995). Polyamines as endogenous growth regulators. In: Davies PJ, editor. Plant Hormones: Physiology, Biochemistry and Molecular Biology. Dordrecht, the Netherlands: Kluwer Academic Publishers, pp. 158–178.
Gamborg OL, Miller RA, Ojima K (1968). Nutrient requirements of suspension cultures of soybean root cells. Exp Cell Res 50: 148–151.
Garrido D, Vicente O, Heberle-Bors E, Isabel Rodriguez-Garcia M (1995). Cellular changes during the acquisition of embryogenic potential in isolated pollen grains of Nicotiana tabacum. Protoplasm 186: 220–230.
Geoffriau E, Kahane R, Martin-Tanguy J (2006). Polyamines are involved in the gynogenesis process in onion. Physiol Plantantarum 127: 119–129.
Gottlieb LD (1973). Enzyme differentiation and phylogeny in Clarkia franciscana, C. rubicunda and C. amoena. J Evol 27: 205–214.
Górecka K, Cvikrova M, Kowalska U, Eder J, Szafrańska K, Górecki R, Janas KM (2007). The impact of Cu treatment on phenolic and polyamine levels in plant material regenerated from embryos obtained in anther culture of carrot. Plant Physiol Biochem 45: 54–61.
Górecka K, Krzyżanowska D, Górecki R (2005a). The influence of several factors on the efficiency of androgenesis in carrot. J Appl Genet 46: 265–269.
Górecka K, Krzyżanowska D, Kiszczak W, Górecki R (2005b). Embryo induction in anther culture of Daucus carota L. Veget Crops Res Bull 63: 25–32.
Górecka K, Krzyżanowska D, Kiszczak W, Kowalska U, Górecki R (2009). Obtaining plants by inducing secondary embryogenesis in carrot androgenetic embryos. Advan Agricul Science Probl Issu 534: 43–50.
Hema BP, Murthy HN (2008). Improvement of in vitro androgenesis in niger using amino acids and polyamines. Biol Plant 52: 121–125.
Jabłońska-Trypuć A, Czerpak R (2007). Biological and therapeutical activity of polyamines. Postępy Fitoterapii1: 32–38 (article in Polish with abstract in English).
Kakkar RK, Nagar PK, Ahuja PS, Rai VK (2000). Polyamines and plant morphogenesis. Biol Plant 43: 1–11.
Kevers C, Gal NL, Monteiro M, Dommes J, Gaspar T (2000). Somatic embryogenesis of Panax ginseng in liquid cultures: a role for polyamines and their metabolic pathways. Plant Growth Reg 31: 209–214.
Kiszczak W, Górecka K, Krzyżanowska D, Kowalska U (2005). Size of flower buds in carrot (Daucus carota L.) as an indicator of a stage of microsporogenesis and its suitability for induction of androgenesis. Veget Crops Res Bull 62: 81–90.
Kumar HG, Altabella AT, Taylor MA, Tiburcio AF (1997). Recent advances in polyamine research. Trends Plant Sci 2: 124–130.
Kumar HG, Ravishankar BV, Murthy HN (2004). The influence of polyamines on androgenesis of Cucumis sativus L. Europ. J Hort Sci 69: 201–205.
Liu ZH, Wang WC, Yan SY (1997). Effect of hormone treatment on callus formation and endogenous indoleacetic acid and polyamine contents of soybean hypocotyl cultivated in vitro. Bot Bull Acad Sin 38: 171–176.
Martinez L, Agüero C, López M, Galmarini C (2000). Improvement of in vitro gynogenesis induction in onion (Allium cepa L.) using polyamines. Plant Sci 156: 221–226.
Niklas A, Butowt R, Jażdżewska E, Majewska-Sawka A (1998). Polyamines in plant cell: synthesis, the mechanisms of action and functions. Advan Cel Biol 25: 33–49.
Özkum Çiner D, Tıpırdamaz R (2002). The effects of cold treatment and charcoal on the in vitro androgenesis of pepper (Capsicum annuum L.). Turk J Bot 26: 131–139.
Paul A, Mitter K, Raychaudhuri S (2009). Effect of polyamines on in vitro somatic embryogenesis in Momordica charantia L. Plant Cell Tiss Organ Cult 97: 303–311.
Purwoko BS, Dewi IS, Usman AW (2001). Poliamina meningkatkan regenerasi tanaman hijau pada kultur antera padi cv Taipei 309. Hayati 8: 117–120 (article in Indonesian with abstract in English).
Redha A, Suleman P (2011). Effects of exogenous application of polyamines on wheat anther cultures. Plant Cell Tiss Organ Cult 105: 345–353.
Savaşkan Ç, Szarejko I, Toker MC (1999). Callus production and plant regeneration from anther culture of some Turkish barley cultivars. Turk J Bot 23: 359–366.
Selander RK, Smith MH, Yang SY, Johnson WE, Gentry JB (1971). Biochemical polymorphism and systematics in the genus Peromyscus. Variation in the old-field mouse (Peromyscus polionotus). Univ Texas Pub 7103: 49–90.
Silveira V, Santa-Catarina C, Tun NN, Scherer GFE, Handro W, Guerra MP, Floh EIS (2006). Polyamine effects on the endogenous polyamine contents, nitric oxide release, growth and differentiation of embryogenic suspension cultures of Araucaria angustifolia (Bert) O Ktze. Plant Sci 171: 91–98.
Sińska I (1986). Polyamines as regulators of plant growth and development. Wiad Botan 30: 9–24 (article in Polish with abstract in English).
Steiner N, Santa-Catarina C, Silveira V, Floh EIS, Guerra MP (2007). Polyamine effects on growth and endogenous hormones levels in Araucaria angustifolia embryogenic cultures. Plant Cell Tiss Organ Cult 89: 55–62.
Szafrańska K, Cvikrova M, Kowalska U, Górecka K, Górecki R, Martinova O, Janas KM (2011). Influence of copper ions on growth, lipid peroxidation, and proline and polyamines content in carrot rosettes obtained from anther culture. Acta Physiol Plant 33: 851–859.
Takeda T, Hayakawa F, Oe K, Matsuoka H (2002). Effects of exogenous polyamine on embryogenic carrot cells. Biochem Eng J 12: 21–28.
Tiainen T (1992). The role of ethylene and reducing agents on anther culture response of tetraploid potato (Solanum tuberosum L.). Plant Cell Rep 10: 604–607.
Tiburcio AF, Campos JL, Figueras X, Besford RT (1993). Recent advances in the understanding of polyamine functions during plant development. Plant Growth Reg 12: 331–340.
Watson MB, Malmberg RL (1996). Regulation of Arabidopsis thaliana L. Heyenh arginine decarboxylase by potassium deficiency stress. Plant Physiol 111: 1077–1083.
Weeden FN, Gottlieb LD (1980). Isolation of cytoplasmic enzymes from pollen. Plant Physiol 66: 400–403.