Çiğdem KÜÇÜK, Merih KIVANÇ, Engin KINACI

Characterization of Rhizobium Sp. Isolated from Bean

Thirty nodule isolates from bean (Phaseolus vulgaris L.) grown in Eskişehir were studied for their physiological and biochemical characteristics. Most isolates produced abundant extracellular polysaccharides, tolerated high salt concentration (5% NaCl), grew at a temperature of 42 °C, and synthesized melanin. They were able to grow at pHs ranging from 3.5 to 9.0. The majority of the isolates showed an intrinsic resistance to the antibiotics chloramphenicol (20 and 50 µg ml-1), erythromycin (30 µg ml-1), kanamycin (10 µg ml-1), and streptomycin (40, 80, and 100 µg ml-1). Plasmid DNA profiles of the isolates were identified and it was determined that 2 isolates contained plasmid DNA from 1 to 3.

Anahtar Kelimeler:

Rhizobium sp., bean (Phaseolus vulgaris L.), isolation, isolate

Characterization of Rhizobium Sp. Isolated from Bean

Keywords:

Rhizobium sp., bean (Phaseolus vulgaris L.), isolation, isolate,

PDF

___

fiehirali S, Gürgün V, Gençtan T et al. Bakteri Aflılaması ve Değiflik Azot Dozlarının Fasulyede (Phaseolus vulgaris L.) Tane Verimi ve Protein Kapsamı Üzerine Etkisi. Toprak Su Arafltırma Raporu, 1981. 2. Jordan DC. Family III. Rhizobiaceae. In: Krieg NR, Holt JG. (Eds.), Bergey’s Manual of Systematic Bacteriology Williams & Wilkins, Baltimore. 1984, pp. 234-242.
Martinez-Romero E, Segovia L, Mercante FM et al. Rhizobium tropici, a novel species nodulating Phaseolus vulgaris L. beans and Leucaena spp. tree. Int J Syst Bacteriol 41: 417-426, 1991.
Segovia L, Young JPW, Martinez-Romero E. Reclassification of American Rhizobium leguminosarum biovar phaseoli type I strains as Rhizobium etli spp. nov. Int J Syst Bacteriol 43: 374-377, 1993. 5. Hardarson G. Methods for enhancing symbiotic nitrogen fixation. Plant and Soil 152: 1-17, 1993.
ETİM, Eskiflehir Tarım İl Müdürlüğü Eskiflehir İli Tarım Master Planı, 2002, pp. 1-12. 7. Graham PH. Some problems of nodulation and symbiotic nitrogen fixation in Phaseolus vulgaris L. Field Crops Res 4: 93-112, 1981. 8. Michiels J, Dombrecht B, Vermeiren N et al. Phaseolus vulgaris L. is a nonselective host for nodulation. FEMS Microbiol. Ecology 26, 193-205, 1998.
Rodriguez-Navarro DN, Buendia AM, Camaso M et al. Characterization of Rhizobium spp. bean isolates from South- West Spain. Soil Biol Biochem 32: 1601-1613, 2000.
Graham P, Sadowsky MJ, Keyser HH et al. Proposed minimal standards for the description of new genera and species of root and stem nodulating bacteria. Int J Syst Bacteriol 41: 582-587, 1991.
El Sheikh EAE and Wood M. Response of chickpea and soybean rhizobia to salt: osmotic and specific ion effects of salts. Soil Biol Biochem 21: 889-985, 1989.
Hashem FM, Swelim DM, Kuykendall LD et al. Identification and characterization of salt and thermo-tolerant Leucaena nodulating Rhizobium strains. Biol Fertil Soils 27: 335-341, 1998.
Lindström K, Lehtomaiki S. Metabolic properties maximum growth temperature and phage sensitivity of Rhizobium sp. (Galega) compared with other fast growing rhizobia. FEMS Microbiol Letters, 50: 277-287, 1988.
Cubo MT, Buendia-Claveria AM, Beringer JE et al. Melanin production by Rhizobium strains. Appl Environ Microbiol 54: 1812-1817, 1988.
Zerhari K, Aurag K, Khbaya B et al. Phenotypic characteristics of rhizobia isolates nodulating Acacia species in the arid and Saharan regions of Morocco. Letters Appl Microbiol 30: 351-357, 2000.
Sajnaga E, Malek W. Numerical taxonomy of Sarothamnus scoparius Rhizobia. Current Microbiol 42: 26-31, 2001.
Hungria M, Andrade DS, Chueira LM et al. Isolation and characterization of new efficient and competitive bean (Phaseolus vulgaris L.) rhizobia in Brazil. Soil Biol. Biochem 32, 1515-1528, 2000.
Hungria M, Vargas MAT. Environmental factors affecting nitrogen fixation in grain legumes in the tropics with an emphasis on Brazil. Field Crops Res 65: 151-164, 2000.
Eckhardt T. A rapid method for the identification of plasmid deoxyribonucleic acid in bacteria. Plasmid 1: 584-588, 1978.
Weaver RW. Stability of plasmids in Rhizobium phaseoli during culture. Soil Biol Biochem 22: 465-469, 1990.