Berrin ÖZÇELIK, Didem Deliorman ORHAN, Nilüfer ORHAN, Fatma ERGUN

Biological activities of Vitis vinifera L. leaves

Bu çalışmada, V. vinifera yapraklarının sulu ekstresinin fraksiyonlanması ile farklı polaritede fraksiyonlar elde edilmiştir (Kloroform, etilasetat, n-butanol ve kalan su fraksiyonları). Bu fraksiyonların antibakteriyal, antifungal, antiviral, antioksidan aktiviteleri ve toplam fenolik içerikleri incelenmiştir. Kloroform fraksiyonu Herpes simplex virüs tip-1 (HSV-1) ve Parainfluenza virüslerine (PIV) karşı ümit verici bir etki göstermiştir. Elde edilen sonuçlar, tüm fraksiyonların gram-pozitif bakterilere (S. aureus ve E. faecalis) gram-negatif bakterilerden (E. coli ve P. aeruginosa) daha belirgin etki gösterdiğini işaret etmektedir. Diğer yandan, fraksiyonlar Candida cinsine (C. albicans ve C. parapsilosis) karşı eşit antifungal aktivite göstermiştir. Ayrıca, V. vinifera fraksiyonlarının antioksidan potansiyeli değişik antioksidan ölçüm yöntemleri (2,2-difenil-2-pikrilhidrazil (DPPH) radikal süpürücü, metal bağlayıcı ve süperoksit radikal temizleyici etki) kullanılarak değerlendirilmiştir. Demir bağlama aktivitesi dışında en yüksek antioksidan aktiviteyi etilasetat fraksiyonu göstermiştir. Diğer yandan, kloroform fraksiyonu en güçlü etkiyi demir bağlama üzerinde göstermiştir. Çalışmamızda, fraksiyonlar ve toplam fenolik içerikleri arasında pozitif bir ilişki görülmüştür. Fakat bu ilişki antimikrobiyal ve antiviral aktivite arasında kurulamamıştır. Sonuç olarak, çalışmada sunulan veriler $CHCI_3$ Fr.’nunun, PIV ve HSV-1’e karşı anlamlı bir aktiviteye sahip olduğunu göstermiştir. Antiviral ve antioksidan aktivite sonuçları, V. vinifera yapraklarının bazı folklorik kullanımlarını destekleyebilir.

Vitis vinifera L. yapraklarının biyolojik aktiviteleri

The fractions of different polarity, namely chloroform, ethylacetate, n-butanol, and remaining water fractions, were fractionated from an aqueous extract of V. vinifera leaves. The antibacterial, antifungal, antiviral, antioxidant activities, and total phenolic content of these fractions were investigated. The chloroform fraction showed a promising effect against Herpes simplex virus type-1 (HSV-1) and Parainfluenza viruses (PIV). The results indicated that all the fractions were more pronounced against gram-positive bacteria (S. aureus and E. faecalis) than against gram-negative bacteria (E. coli and P. aeruginosa). On the other hand, the fractions showed equal antifungal activity against the genus Candida (C. albicans and C. parapsilosis). Additionally, the antioxidative potential of different fractions of V. vinifera was evaluated using various antioxidant measurements, i.e. 2,2-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging, metal chelating, and superoxide radical scavenging effects. The ethylacetate fraction possessed the highest antioxidant activities except in the case of iron chelation. On the other hand, the chloroform fraction showed the best performance in the iron chelation assay. In our study, the analysis of the relationship between the antioxidant activity of the fractions and their phenolic content showed a positive correlation coefficient. However, such a correlation was not found for the antimicrobial and antiviral properties. In conclusion, the data presented in the study demonstrated that $CHCI_3$ Fr. produced significant activity against PIV and HSV-1. The results of the antiviral and antioxidant activities supported the utilization of V. vinifera leaves in folk medicine.

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