An anti-Shigella dysenteriae bacteriocin from Pediococcus pentosaceus MTCC 5151 cheese isolate

A cheese isolate Pediococcus pentosaceus lactic acid bacterium, which has been deposited at the Microbial Type Culture Collection Centre Chandigarh with the accession number MTCC 5151, was tested for anti-Shigella dysenteriae activity and the bacteriocin was characterized. The protein band was observed with tricine sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) as a single band with a molecular mass of 23 kDa. This is a new and novel bacteriocin that inhibits S. dysenteriae and has not yet been reported from P. pentosaceus. It was purified on a Sephacryl column and the active fraction specific for anti-Shigella dysenteriae with 23 kDa was found and confirmed via liquid chromatography mass spectrometry (LC-MS). The effect of various physical parameters on bacteriocin activity was also studied, with the optimum conditions being determined at a pH level of 5.5 with an 18-h-grown culture.

Anahtar Kelimeler:

Key words: Bacteriocin, Pediococcus pentosaceus, purification, anti-Shigella dysenteriae

An anti-Shigella dysenteriae bacteriocin from Pediococcus pentosaceus MTCC 5151 cheese isolate

Keywords:

Key words: Bacteriocin, Pediococcus pentosaceus, purification, anti-Shigella dysenteriae,

PDF

___

Sur D, Ramamurthy T, Deen J et al. Shigellosis: challenges and management issues. Ind J Med Res 120: 454-462, 2004.
Lozano NJC, Meyer JN, Sletten K et al. Purifi cation and amino acid sequence of a bacteriocin produced by Pediococcus acidilactici. J Gen Microbiol 138: 1985-1990, 1992.
Schved F, Lalazar A, Henis Y et al. Purifi cation, partial characterization and plasmid linkage of pediocin SJ-1 a bacteriocin produced by Pediococcus acidilactici. J Appl Bacteriol 74: 67-77, 1993.
Motlagh AM, Bhunia AK, Szostek F et al. Nucleotide and amino acid sequence of pap-gene (pediocin AcH production) in Pediococcus acidilactici H. Lett Appl Microbiol 15: 45-48, 1992.
Daba H, Lacroix C, Huang J et al. Simple method of purifi cation and sequencing of a bacteriocin produced by Pediococcus acidilactici UL 5. J Appl Bacteriol 77: 682-688, 1994.
Lowry OH, Rosebrough NJ, Farr AL et al. Protein measurement with the folin phenol reagent. J Biol Chem 193: 265-275, 1951.
Bhunia AK, Johnson MC, Ray B. Direct detection of an antimicrobial peptide of Pediococcus acidilactici in sodium dodecyl sulfate-polyacrylamide gel electrophoresis. J Ind Microbiol 2: 319-322, 1987.
Schagger H, Jagow GV. Tricine–sodium dodecyl sulfate– polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa. Anal Biochem 166: 368-379, 1987.
Biswas SR, Ray P, Johnson MC et al. Infl uence of growth conditions on the production of a bacteriocin Pediocin AcH, Pediococcus acidilactici H. Appl Environ Microbiol 57: 1265- 1267, 1991.
Elegado FB, Kim WJ, Kwon DY. Rapid purifi cation, partial characterization and antimicrobial spectrum of the bacteriocin, pediocin AcM from Pediococcus acidilactici M. Int J Food Microbiol 37: 1-11, 1997.
Bhunia AK, Johnson MC, Ray B et al. Mode of action of pediocin AcH from Pediococcus acidilactici H on sensitive bacterial strains. J Appl Bacteriol 70: 25-33, 1991.
Joosten HMLJ, Numez M. Adsorption of nisin and enterocin 4 to polypropylene and glass surfaces and its prevention by Tween 80. Lett Appl Microbiol 21: 389-392, 1995.
Gonzales CF, Kunka BS. Plasmid associated bacteriocin production and sucrose fermentation in Pediococcus acidilactici. Appl Environ Microbiol 53: 2534-2538, 1987.
Pucci MJ, Vedamuthu ER, Kunka BS et al. Inhibition of Listeria monocytogenes by using bacteriocin PA-I produced by Pediococcus acidilactici PA C1.0. Appl Environ Microbiol 54: 2349-2353, 1988.
Choi SY, Beuchat LR. Growth inhibition of Listeria monocytogenes by a bacteriocin of pediococcus acidilactici M during fermentation of kimchi. Food Microbiol 11: 301-307, 1994.
Chikindas ML, Garcia-Garcera MJ, Driessen AJ et al. Pediocin PA-1, a bacteriocin from pediococcus acidilactici PAC 1.0 forms hydrophilic pores in the cytoplasmic membrane of target cells. Appl Environ Microbiol 59: 3577-3584, 1988.
Pieter VS, Jacobus S, Cornelis LP. Int.pat. No. WO/1993/018179. Bacteriocin from Pediococcus cerevisae (1993).
Jamuna M, Jeevaratnam K. Isolation and partial characterization of bacteriocins from pediococcus species. Appl Microbiol Biotechnol 65: 433-439, 1985.
Piva A, Headon DR. Pediocin A, A bacteriocin produced by P. pentosaceous FBB61. Microbiology 140: 697-702, 1994.
Wu CW, Yin LJ, Jiang ST. Purifi cation and characterization of bacteriocin from pediococcus pentosaceus ACCEL. J Agric Food Chem 54: 1146-1151, 2004.