Using smoke solutions in grass pea (Lathyrus sativus L.) to improve germination and seedling growth and reduce toxic compound ODAP
Using smoke solutions in grass pea (Lathyrus sativus L.) to improve germination and seedling growth and reduce toxic compound ODAP
The effects of smoke solutions derived from wheat straw on the germination, seedling growth characteristics, and biochemicalcontent of grass pea (Lathyrus sativus L.) were examined in this study. The smoke solutions were applied to petri and pot media atdifferent concentrations. Both experiments were conducted in a randomized plot design with 3 replications. In the pot experiments,the smoke solutions were applied through the initial irrigation at 5 different concentrations (1%, 5%, 10%, 20%, and 40%) while 6concentrations were used for petri experiments (0.5%, 1%, 2%, 4%, 8%, 10%) and results were compared to a control (distilled water).The smoke solutions increased root and shoot growth but decreased the germination rate and speed of grass pea in both media. Ingeneral, there were positive outcomes for the investigated traits with moderate concentrations of solution, but negative outcomes withlow and high concentrations. The smoke solution treatments improved the shoot dry weighs, K, and protein contents, but decreased theacid detergent fiber and neutral detergent fiber contents and the toxic component oxalyldiaminopropionic acid (ODAP). Accordingly,the smoke solutions stimulated the development of grass pea and promising outcomes were achieved in terms of high protein and lowODAP contents, which are the main targets of grass pea breeding studies. It was concluded, based on the present findings, that smokesolutions derived from wheat straw could be used as an environmentally friendly practice in grass pea culture to increase yield andquality. However, it should be kept in mind that concentration was a critical issue for ensuring the expected outcomes.
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- Aletor VA, Abd El Moneim AM, Goodchild AV (1994). Evaluation
of the seeds of selected lines of three Lathyrus species for b-Noxalylyamino-L-alanine (BOAA), tannins, trypsin inhibitor
activity and certain in-vitro characteristics. J Sci Food Agric
65: 143-151.
- Aslam MM, Akhter A, Jamil M, Khatoon A, Malook I, Rehman S
(2014). Effect of plant-derived smoke solution on root of
ıpomoea marguerite cuttings under cobalt stress. J Bio Mol Sci
2: 6-11.
- Basaran U, Acar Z, Karacan M, Onar AT (2013). Variation and
correlation of morpho-agronomic traits and biochemical
contents (protein and βOdap) in Turkish grass pea (Lathyrus
sativus L.) landraces. Turkish Journal of Field Crops 18: 166-
173.
- Basaran U, Mut H, Gulumser E, Copur Dogrusoz M (2016).
Evaluation of Turkish grass pea (Lathyrus sativus L.) collections
for its agronomic characters with a special reference to ODAP
content. Legume Res 39: 876-882.
- Campbell CG (1997). Grass Pea. Lathyrus sativus L: Promoting the
Conservation and Use of Underutilized and Neglected Crops.
Vol. 18. Rome, Italy: Institute of Plant Genetics and Crop Plant
Research /International Plant Genetic Resources Institute.
Catav SS, Bekar I, Ates BS, Ergan G, Oymak F, Ulker ED, Tavsanoglu
C (2012). Germination response of five eastern Mediterranean
woody species to smoke solutions derived from various plants.
Turk J Bot 36: 480-487.
- Czabator FJ (1962). Germination value: an index combining speed
and completeness of pine seed germination. Forest Sci 8:386-
395.
- De Lange JH, Boucher C (1990). Autecological studies on Audouinia
capitata (Bruniaceae). I. Plant-derived smoke as a seed
germination cue. S Afr J Bot 56: 700-703.
- Demir I, Ozuaydin F, Yasar J, Van Staden J (2012) Effect of smokederived butenolide priming treatment on pepper and salvia
seeds in relation to transplant quality and catalase activity.
South Afr J Bot 78: 83-87.
- Drewes FE, Smith MT, Van Staden J (1995). The effect of a plantderived smoke extract on the germination of light-sensitive
lettuce seed. Plant Growth Regul 16: 205-209.
- Ghebrehiwot HM, Kulkarni GM, Bairu MW, Van Staden J (2013).
Plant-derived aerosol-smoke and smoke solutions influence
agronomic performances of the traditional cereal crop, tef.
Exp Agric 49: 244-255.
- Ghebrehiwot HM, Kulkarni GM, Kirkman KP, Van Staden J (2009).
Smoke solutions and temperature influence the germination
and seedling growth of South African mesic grassland species.
Rangeland Ecol Manag 62: 572-578.
- Ghebrehiwot HM, Kulkarni GM, Kirkman KP, Van Staden J (2011).
Germination activity of smoke residues in soils following a fire.
S Afr J Bot 77: 718-724.
- Ghebrehiwot HM, Kulkarni GM, Kirkman KP, Van Staden J (2012).
Smoke and heat: influence on seedling emergence from the
germinable soil seed bank of mesic grassland in South Africa.
Plant Growth Regul 66: 119-127.
- Girma A, Tefera B, Dadi L (2011). Grass pea and neurolathyrism:
farmers’ perception on its consumption and protective measure
in North Shewa, Ethiopia. Food Chem Toxicol 49: 668-672.
- Govindaraj M, Masilamani P, Albert AV, Bhaskaran M (2016).
Plant derived smoke stimulation for seed germination and
enhancement of crop growth: a review. Agr Rev 37: 87-100.
- Hanbury CD, Siddique KHM, Galwey NW, Cocks PS (1999).
Genotype-environment interaction for seed yield and ODAP
concentration of Lathyrus sativus L. and L. cicero L. in
Mediterranean-type environments. Euphytica 110: 445-460.
- Hardegree SP, Van Vactor SS (2000). Germination and emergence of
primed grass seeds under field and simulated-field temperature
regimes. Ann Bot London 85: 379-390.
- Iqbal M, Asif S, Ilyas N, Ul-Hassan F, Raja NI, Hussain M, Ejaz H,
Saira M (2017). Smoke produced from plants waste material
elicits growth of wheat (Triticum aestivum L.) by improving
morphological, physiological and biochemical activity.
Biotechnol Rep (Amst) 13: 35-44.
- Kamran M, Imran Q, Khatoon A, Lee I, Rehman S (2013). Effect of
plant extracted smoke and reversion of abscisic acid stress on
lettuce. Pak J Bot 45: 1541-1549.
- Kulkarni MG, Ascough GD, Van Staden J (2007). Effects of foliar
applications of smoke-water and a smoke-isolated butenolide
on seedling growth of okra and tomato. HortScience 42: 179-
182.
- Kumar S, Bejiga G, Ahmed S, Nakkoul H, Sarker A (2011). Genetic
improvement of grass pea for low neurotoxin (beta-ODAP)
content. Food Chem Toxicol 49: 589-600.
- Light ME, Gardner MJ, Jäger AK, Van Staden J (2002). Dual
regulation of seed germination by smoke solutions. Plant
Growth Regul 37: 135-141.
- Light ME, Van Staden J, Bornman CH (2004). The potential of smoke
in seed technology. S Afr J Bot 70: 97-101.
- Massawe F, Mayes S, Cheng A (2016). Crop diversity: an unexploited
treasure trove for food security. Trends Plant Sci 21: 365-368.
- Mehta SL, Ali K, Barna KS (1994). Somaclonal variation in a food
legume Lathyrus sativus. J. Plant Biochem 3: 73-77.
- Polignano GB, Bisignano V, Tomaselli V, Uggenti P, Alba V, Della
Gatta C (2009). Genotype x environment interaction in grass
pea (Lathyrus sativus L.) lines. Int J Agron 2009: 1-7.
- Rao S, Adiga P, Sarma P (1964). The isolation and characterization of
β-N-oxalyl-L-α, β-diaminopropionic acid: a neurotoxin from
the seeds of Lathyrus sativus. Biochemistry 3: 432-436.
- Rao SLN (1978). A sensitive and specific colorimetric method for
determination of α, β-diaminopropionic acid and Lathyrus
sativus neurotoxin. Anal. Biochem 86: 386-395.
- Ren L, Bai YG (2016). Smoke originated from different plants has
various effects on germination and seedling growth of species
in fescue prairie. Botany 94: 1141-1150.
- Rybiñski W, Blaszcak W, Fornal J (2006). Seed microstructure and
genetic variation of characters in selected grass-pea mutants
(Lathyrus sativus L.). Agrophysics 20: 317-326.
- Van Staden J, Jager AK, Light ME, Burger BV (2004). Isolation of the
major germination cue from plant-derived smoke. S Afr J Bot
70: 654-659.
- Waheed MA, Muhammad J, Muhammad DK, Shakirullah KS, UrRehman S (2016). Effect of plant-derived smoke solutions on
physiological and biochemical attributes of maize (Zea mays
L.) under salt stress. Pak J Bot 48: 1763-1774.
- Wang F, Chen X, Chen Q, Qin X, Li Z (2000). Determination of
neurotoxin b-N oxalyl-L-a,b-diaminopropionic acid and
nonprotein amino acids in Lathyrus sativus by precolumn
derivatization with 1-fluoro-2,4-dinitrobenzene. J Chromatogr
A 883: 113-118.
- Yan ZY, Spencer PS, Li ZX, Liang YM, Wang YF, Wang CY, Li FM
(2006). Lathyrus sativus (grass pea) and its neurotoxin ODAP.
Phytochemistry 67: 107-121.
- Zhao L, Chen XG, Hu ZD, Li QF, Chen Q, Li ZX (1999). Analysis
of β-N-oxalyl-l-α, β-diaminopropionic acid and homoarginine
in Lathyrus sativus by capillary zone electrophoresis. J
Chromatogr A 857: 295-302.
- Zhelyazkova T, Pavlov D, Delchev G, Stoyanova A (2016).
Productivity and yield stability of six grain legumes in the
moderate climatic conditions of Bulgaria. Scientific Papers A
Agronomy 9: 478-487.