İsmail BAYSAL, Süleyman AKBULUT, Beşir YÜKSEL, Metin SERİN, Mahir ERDEM

Pathogenicity of Bursaphelenchus mucronatus in pine seedlings under greenhouse conditons

Özet: Çam odun nematoduyla {Bursaphelenchus xylophilus, karantina listesindeki bir zararlı) benzerlikleri çok olan Bursaphelenchus mucronatus türü Türkiye'nin farklı illerinde kurumuş olan bazı çam ağaçlarından elde edilmiştir. B. mucronatus'un bu kurumalardaki rolünün belirlenmesi için en çok yayılışa sahip üç çam türünün fidanları kullanılarak sera koşullarında patojenlik testi yapılmıştır. Her ağaç türünden 30 adet fidan kullanılmıştır. Aşılamalarda her fidan için 0.5 mi distile edilmiş su içersinde 1000-1100 adet nematod kullanılmıştır. Her ağaç türünden 20 adet fidana kontrol amaçlı olarak 0.5 mi distile edilmiş su inoküle edilmiştir. İlk tam kuruma, denemenin 5. haftasında gerçekleşmiştir. En yüksek ölüm yüzdesi (% 83) Pinus sylvestris fidanlarında ve takiben P. nigra fidanlarında (% 47) görülmüştür. P. brutia fidanlarından sadece iki tanesi ölmüştür (% 7). Bu sonuçlar, B. mucronatus'un P. sylvestris fidanlarında yüksek oranda öldürücü olduğunu göstermektedir.

Bursaphelenchus mucronatus' un Sera koşulları altında Çam fidanlarındaki patojenliği

Abstract: Bursaphelenchus mucronatus, a species closely related to the pinewood nematode (Bursaphelenchus xylophilus, a quarantine pest), was isolated from several wilted pine trees in different provinces of Turkey. To understand the role of B. mucronatus in these wilting cases, the pathogenicity of B. mucronatus on 3-year-old seedlings of the 3 pine species most widespread in Turkey was investigated under greenhouse conditions. In all, 30 seedlings of each tree species were used for nematode inoculation. For inoculation, 1000-1100 nematodes in 0.5 ml of distilled water were used per seedling. An additional 20 seedlings of each tree species were inoculated with an equal amount of distilled water and served as controls. The first complete wilting case was observed in the fifth week of the experiment. The highest rate of mortality occurred in Pinus sylvestris seedlings (83%), followed by P. nigra (47%). Only 2 seedlings of P. brutia wilted (7%). These results suggested that B. mucronatus was highly virulent to P. sylvestris seedlings.

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