Paecilomyces spp.’nin Sera Beyazsine¤ine, Trialeurodes vaporariorum, Patojenitesi ve Fungal Enfeksiyon Geliflimi Üzerine Baz› Gözlemler

Bu çal›flma dokuz Paecilomyces fumosoroseus ile bir Paecilomyces lilacinus izolat›n›n sera beyazsine¤i (Trialeurodes vaporariorum)’ni enfekte etme oranlar›n› belirlemek için yürütülmüfltür. Ayr›ca, fungal etmen P. fumosoroseus’un T. vaporariorum üzerinde gerçeklefltirdi¤i enfeksiyon aflamalar› ›fl›k ve elektron mikroskoplar› kullan›larak gözlemlenmifltir. Biyotestler 2. dönemnimfler üzerine fungal spor süspansiyonu inokule edilerek yürütülmüfltür. Spor suspansiyonunun inokule edilmesini takip eden 3. ve 6. günlerde ölümler kaydedilmifltir. Bütün Paecilomyces izolatlar›n›n etkinlikleri inkübasyon süresi uzat›ld›kça art›rm›flt›r. Test edilen izolatlardan 7 tanesi sera beyazsine¤i nimfleri üzerinde önemli oranda ölümlere neden olmufltur. ‹zolatlar aras›nda en yüksek virulens gösteren 2658, 4400, 4406, 4408 ve 4415 nolu izolatlar inokulasyondan alt› gün sonra % 70’i aflan ölümlere neden olmufltur. Enfeksiyonun geliflim aflamalar› suni olarak enfekte edilmifl nimfler üzerinde 12 saat aral›klar ile takip edilmifltir. Fungusun sera beyazsine¤ini enfekte etme aflamalar› di¤er böceklerde funguslar taraf›ndan gerçeklefltirilen enfeksiyonlarla benzerlik göstermektedir. Çal›flman›n sonuçlar› fungusun konukçusunu k›sa sürede öldürdü¤ünü, sera beyazsine¤ine karfl› patojenik oldu¤unu ve bu nedenle sera beyazsine¤inin seralarda mücadelesinde biyokontrol etmeni olarak bu fungus üzerinde daha fazla çal›flman›n gereklili¤ini göstermifltir.

Anahtar Kelimeler:

Paecilomyces fumosoroseus, Paecilomyces lilacinus, entomopatojenik fungus, Trialeurodes vaporariorum, sera beyazsine¤i, biyolojik mücadele

Pathogenicity of Paecilomyces spp. to the Glasshouse Whitefly, Trialeurodes vaporariorum, with Some Observations on the Fungal Infection Process

This study was carried out to determine the infection rates of 9 Paecilomyces fumosoroseus isolates and 1 Paecilomyces lilacinus isolate on the glasshouse whitefly (GWF), Trialeurodes vaporariorum. Furthermore, the infection progress of P. fumosoroseus was observed on the insect using light and scanning electron microscopes. Bioassays were carried out on second instar nymphs by the application of a spore suspension of each isolate. The mortality data were recorded 3 and 6 days after the application. The effect of Paecilomyces isolates increased as the incubation time was extended. Seven isolates caused significant mortality to the GWF nymphs. The most virulent isolates, 2658, 4400, 4406, 4408 and 4415, killed over 70% of the inoculated nymphs 6 days after inoculation. The development of infection was monitored on artificially infected nymphs at 12-h intervals. Infection by the fungus on the GWF was similar to those reported for other fungus-insect cases. The results of this study showed that the fungus was pathogenic to T. vaporariorum, rapidly killing its host, and thus further studies are required to determine the potential use of the fungus as a biocontrol agent against GWF in greenhouses.

Keywords:

Paecilomyces fumosoroseus, Paecilomyces lilacinus, Entomopathogenic fungus, Trialeurodes vaporariorum, Glasshouse whitefly, biological control,

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