Mustafa CÜCE, Ersan BEKTAŞ, Atalay SÖKMEN

Micropropagation of Vaccinium arctostaphylos L. via lateral-bud culture

The aim of this study was to determine the most suitable growth medium for rapid and efficient micropropagation of Vaccinium arctostaphylos L. (Ericaceae) via lateral-bud cultures. According to preliminary studies, the best explant collection time was late April and May. Accordingly, cuttings were taken from naturally growing V. arctostaphylos populations, and lateral shoot buds were used as explant sources. Anderson's rhododendron medium (AN), McCown's woody plant medium (WPM), and Murashige and Skoog (MS) basal medium, each supplemented with zeatin/indole-3-butyric acid (IBA) (1.0/0.1 mg L-1), were tested to determine the best basal medium for multiple shoot formation; WPM was the most effective basal medium for this purpose. Subsequent shoot multiplication and development studies were carried out with WPM supplemented with an auxin, IBA (0.1 mg L-1), and 2 different cytokinins, zeatin and thidiazuron (TDZ), in various concentrations (0.5, 1.0, and 2.0 mg L-1, respectively). After a 6-week photoperiod (16/8 h, light/ dark) application, zeatin/IBA combinations were found to be the most suitable for shoot multiplication and growth. The highest shoot length (106.53%) was obtained from the medium supplemented with 1.0/0.1 mg L-1 zeatin/IBA, while the highest increases in leaf number (141.89%) and multiple shoot proliferation (10.55-fold) were obtained from medium supplemented with 2.0/0.1 mg L-1 zeatin/IBA. WPM was also employed as a rooting medium supplemented with different concentrations of IBA (from 0.5 mg L-1 to 8.0 mg L-1) and a 16/8 photoperiod or a completely dark regime. At this stage, the medium supplemented with 0.5 mg L-1 IBA was the best rooting growth regulator with 100% rooting success (16/8 photoperiod). Rooted plantlets transplanted into peat and perlite (2:1) substrate were subsequently acclimatized under climate chamber conditions.

Anahtar Kelimeler:

Key words: Indole-3-butyric acid, shoot-bud culture, thidiazuron, Vaccinium arctostaphylos, whortleberry, zeatin

Micropropagation of Vaccinium arctostaphylos L. via lateral-bud culture

Keywords:

Key words: Indole-3-butyric acid, shoot-bud culture, thidiazuron, Vaccinium arctostaphylos, whortleberry, zeatin,

PDF

___

Debnath SC, McRae KB (2001) In vitro culture of lingonberry (Vaccinium vitis-idaea L.): the influence of cytokinins and media types on propagation. Small Fruits Rev 1: 3–19.
Debnath SC, McRae KB (2005) A one-step in vitro cloning procedure for cranberry (Vaccinium macrocarpon Ait.): the influence of cytokinins on shoot proliferation and rooting. Small Fruits Rev 4: 57–75.
Eccher T, Noe N (1989) Comparison between 2iP and zeatin in the micropropagation of highbush blueberry (Vaccinium corymbosum). ISHS Acta Hortic 241: 185–190.
Jaakola L, Maatta K, Pirttila AM, Törrönen N, Karenlampi S, Hohyola A (2002) Expression of genes involved in anthocyanin biosynthesis in relation to anthocyanin, proanthocyanidin, and flavonol levels during bilberry fruit development. Plant Physiol 130: 729–739.
Lloyd G, McCown B (1980) Commercially feasible micropropagation of mountain laurel (Kalmia latifolia) by use of shoot tip culture. Comb Proc Intl Plant Prop Soc 30: 421–427.
Marcotrigiano M, McGlew SP, Hackett G, Chawla B (1996) Shoot regeneration from tissue-cultured leaves of the cranberry (Vaccinium macrocarpon) plant cell. Plant Cell Tissue Organ Cult 44: 195–199.
Meiners J, Schwab M, Szankowski I (2007) Efficient in vitro regeneration systems for Vaccinium species. Plant Cell Tissue Organ Cult 89: 169–176.
Morrison S, Smagula JM (2000) Morphology, growth, and rhizome development of Vaccinium angustifolium Ait. seedlings, rooted softwood cuttings, and micropropagated planted. HortScience 35: 738–741.
Ostrolucká MG, Libiaková G, Ondrušková A, Gajdošová A (2004) In vitro propagation of Vaccinium species. Acta Univ Latv 676: 207–212.
Reed BM, Abdelnour-Esquivel A (1991) The use of zeatin to initiate in vitro cultures of Vaccinium species and cultivars. HortScience 26: 1320–1322.