Hatice OGUTCU, Yasemin ERBEY, Medine GÜLLÜCE, Burak ALAYLAR4, , Mehmet KARADAYI, Selma SEZEN, Mahmut ERBEY

Isolation and identification of Bacillus pumilus YHH-2, a potential pathogen to the alfalfa weevil (Hypera postica Gyllenhal)

Alfalfa weevil (Hypera postica Gyllenhal, 1813) samples were collected from different locations in Kirsehir, Turkey, during May-August in 2014. They were immediately transferred to the research laboratory and dissected under aseptic conditions. Then serial dilutions were prepared from digestive tract samples and the spread plate technique was used for obtaining pure cultures. The conventional and molecular methods that include morphological and physiological examinations, biochemical tests, 16S rDNA sequencing, and the basic local alignment search tool (BLAST) were used for identification of the bacterial isolates. According to the findings of the present study, the YHH-2 isolate was found as the main pathogenic bacterium that dominates the digestive tract microflora of the alfalfa weevil. 16S rDNA sequencing and the BLAST data revealed that YHH-2 showed 100% similarity to Bacillus pumilus, recently described as an entomopathogenic strain. In conclusion, the results of the present study are important due to showing for the first time the presence of Bacillus pumilus Y HH-2 in the digestive tract of the alfalfa weevil and it may be used as a potential biocontrol agent against the harmful effects of this insect species.

Keywords:

Alfalfa weevil, Bacillus pumilus, biocontrol, entomopathogenic bacterium,

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Cawoy H, 2011, PESTICIDES IN THE MODERN WORLD - PESTICIDES USE AND MANAGEMENT, P273
Dharne M, 2006, J BIOSCIENCES, V31, P293, DOI 10.1007/BF02704100
Earl AM, 2007, J BACTERIOL, V189, P1163, DOI 10.1128/JB.01343-06
Efil L, 2018, TURKISH J AGR NAT SC, V5, P86
Gogusgeren N, 2009, DETERMINATION STRAIN
Handtke S, 2014, PLOS ONE, V9, DOI 10.1371/journal.pone.0085625
Iwase S, 2015, BIOL INVASIONS, V17, P3639, DOI 10.1007/s10530-015-0985-1
JOHANSEN CA, 1983, ENVIRON ENTOMOL, V12, P1513, DOI 10.1093/ee/12.5.1513
KINGSLEY PC, 1993, ENVIRON ENTOMOL, V22, P1234, DOI 10.1093/ee/22.6.1234
Kloepper JW, 2004, PHYTOPATHOLOGY, V94, P1259, DOI 10.1094/PHYTO.2004.94.11.1259
Lu F, 2013, ENVIRON ENTOMOL, V42, P874, DOI 10.1603/EN13111
Nielsen P, 1997, FEMS MICROBIOL ECOL, V22, P183, DOI 10.1016/S0168-6496(96)00089-X
Ozmen M, 2009, EFFECTS INSECTICIDE .
Pellissier ME, 2017, J INTEGR PEST MANAG, V8, DOI 10.1093/jipm/pmw018
Pitts-Singer Theresa L., 2008, P105 .
Pu YC, 2016, J APPL ENTOMOL, V140, P617, DOI 10.1111/jen.12293
Pu YC, 2017, PEST MANAG SCI, V73, P1494, DOI 10.1002/ps.4485
Reddy GVP, 2016, TOXICOL REP, V3, P473, DOI 10.1016/j.toxrep.2016.05.003
Rishad KS, 2017, PESTIC BIOCHEM PHYS, V137, P36, DOI 10.1016/j.pestbp.2016.09.005
Seyma S.K, 2014, ERCIYES U J I SCI TE, V30, P338
Slepecky RA, 2006, PROKARYOTES: A HANDBOOK ON THE BIOLOGY OF BACTERIA, VOL 4, THIRD EDITION, P530, DOI 10.1007/0-387-30744-3_16
Summers C. G., 1998, Integrated Pest Management Reviews, V3, P127, DOI 10.1023/A:1009654901994
Talwar N, 2015, CANAKKALE ONSEKIZ MA, V3, P9
Temiz A, 2010, GENEL MIKROBIYOLOJI .
Tetik Z, 2007, MICROORGANISMS ISOLA .
Tuatay N, 1952, PLANT PROTECTION B, V4, P10
Wilson K., 1997, CURRENT PROTOCOLS MO, V56, P241