Induction of Phytoalexin Accumulation in Broad Bean (Vicia faba L.) Cotyledons Following Treatments with Biotic and Abiotic Elicitors

Broad bean (Vicia faba) cotyledons that were inoculated artificially with fungal pathogens or exposed to various abiotic agents were analysed for phytoalexin production. Biotic elicitors, such as Botrytis cinerea and B. allii, and abiotic elicitors, such as ultraviolet (UV) radiation (254 nm) and freezing-thawing, were used to induce phytoalexin accumulation. Wyerone and other wyerone derivatives were the major phytoalexins responding in broad bean cotyledons. The quantities of wyerone within elicitor-treated tissues were examined by thin layer chromatography. The highest amount of wyerone was induced by B. cinerea (943 mg/g fresh weight). Treatment of cotyledons with UV radiation (452 mg/g f.wt), B. alli (325 mg/g f.wt) and freezing-thawing (288 mg/g f.wt) also caused considerable activation of the phytoalexin synthesis. Cell necrosis and wyerone accumulation were closely associated, and the highest concentration of wyerone was in tissue bearing brown lesions. Only very low concentrations of wyerone accumulated at sites of mechanical damage. The results indicate that the presence of both damaged and healthy tissues is necessary for phytoalexin production.

Induction of Phytoalexin Accumulation in Broad Bean (Vicia faba L.) Cotyledons Following Treatments with Biotic and Abiotic Elicitors

Broad bean (Vicia faba) cotyledons that were inoculated artificially with fungal pathogens or exposed to various abiotic agents were analysed for phytoalexin production. Biotic elicitors, such as Botrytis cinerea and B. allii, and abiotic elicitors, such as ultraviolet (UV) radiation (254 nm) and freezing-thawing, were used to induce phytoalexin accumulation. Wyerone and other wyerone derivatives were the major phytoalexins responding in broad bean cotyledons. The quantities of wyerone within elicitor-treated tissues were examined by thin layer chromatography. The highest amount of wyerone was induced by B. cinerea (943 mg/g fresh weight). Treatment of cotyledons with UV radiation (452 mg/g f.wt), B. alli (325 mg/g f.wt) and freezing-thawing (288 mg/g f.wt) also caused considerable activation of the phytoalexin synthesis. Cell necrosis and wyerone accumulation were closely associated, and the highest concentration of wyerone was in tissue bearing brown lesions. Only very low concentrations of wyerone accumulated at sites of mechanical damage. The results indicate that the presence of both damaged and healthy tissues is necessary for phytoalexin production.

Kaynak Göster

Bibtex @ { tbtkagriculture138639, journal = {Turkish Journal of Agriculture and Forestry}, issn = {1300-011X}, eissn = {1303-6173}, address = {}, publisher = {TÜBİTAK}, year = {2002}, volume = {26}, pages = {343 - 348}, doi = {}, title = {Induction of Phytoalexin Accumulation in Broad Bean (Vicia faba L.) Cotyledons Following Treatments with Biotic and Abiotic Elicitors}, key = {cite}, author = {Soylu, Soner} }
APA Soylu, S . (2002). Induction of Phytoalexin Accumulation in Broad Bean (Vicia faba L.) Cotyledons Following Treatments with Biotic and Abiotic Elicitors . Turkish Journal of Agriculture and Forestry , 26 (6) , 343-348 . Retrieved from https://dergipark.org.tr/tr/pub/tbtkagriculture/issue/11642/138639
MLA Soylu, S . "Induction of Phytoalexin Accumulation in Broad Bean (Vicia faba L.) Cotyledons Following Treatments with Biotic and Abiotic Elicitors" . Turkish Journal of Agriculture and Forestry 26 (2002 ): 343-348 <https://dergipark.org.tr/tr/pub/tbtkagriculture/issue/11642/138639>
Chicago Soylu, S . "Induction of Phytoalexin Accumulation in Broad Bean (Vicia faba L.) Cotyledons Following Treatments with Biotic and Abiotic Elicitors". Turkish Journal of Agriculture and Forestry 26 (2002 ): 343-348
RIS TY - JOUR T1 - Induction of Phytoalexin Accumulation in Broad Bean (Vicia faba L.) Cotyledons Following Treatments with Biotic and Abiotic Elicitors AU - Soner Soylu Y1 - 2002 PY - 2002 N1 - DO - T2 - Turkish Journal of Agriculture and Forestry JF - Journal JO - JOR SP - 343 EP - 348 VL - 26 IS - 6 SN - 1300-011X-1303-6173 M3 - UR - Y2 - 2021 ER -
EndNote %0 Turkish Journal of Agriculture and Forestry Induction of Phytoalexin Accumulation in Broad Bean (Vicia faba L.) Cotyledons Following Treatments with Biotic and Abiotic Elicitors %A Soner Soylu %T Induction of Phytoalexin Accumulation in Broad Bean (Vicia faba L.) Cotyledons Following Treatments with Biotic and Abiotic Elicitors %D 2002 %J Turkish Journal of Agriculture and Forestry %P 1300-011X-1303-6173 %V 26 %N 6 %R %U
ISNAD Soylu, Soner . "Induction of Phytoalexin Accumulation in Broad Bean (Vicia faba L.) Cotyledons Following Treatments with Biotic and Abiotic Elicitors". Turkish Journal of Agriculture and Forestry 26 / 6 (Haziran 2002): 343-348 .
AMA Soylu S . Induction of Phytoalexin Accumulation in Broad Bean (Vicia faba L.) Cotyledons Following Treatments with Biotic and Abiotic Elicitors. Turkish Journal of Agriculture and Forestry. 2002; 26(6): 343-348.
Vancouver Soylu S . Induction of Phytoalexin Accumulation in Broad Bean (Vicia faba L.) Cotyledons Following Treatments with Biotic and Abiotic Elicitors. Turkish Journal of Agriculture and Forestry. 2002; 26(6): 343-348.
IEEE S. Soylu , "Induction of Phytoalexin Accumulation in Broad Bean (Vicia faba L.) Cotyledons Following Treatments with Biotic and Abiotic Elicitors", Turkish Journal of Agriculture and Forestry, c. 26, sayı. 6, ss. 343-348, Haz. 2002