Identification of Root-Knot Nematodes in the Mediterranean Region of Turkey by Using rDNA and mtDNA Markers

Fragments containing the internal transcribed spacer (ITS) ribosomal rDNA were amplified from Meloidogyne incognita, M. javanica, M. arenaria and M. hapla by polymerase chain reaction (PCR). Only digestion of the ITS region with the enzyme RsaI significantly distinguished M. hapla from other species, while other enzymes, EcoRI and BamHI, were not effective in distinguishing these nematode species. PCR was also done with mitocondrial DNA (mtDNA) markers for species discrimination. The obtained amplification product of 600 bp was digested with HinfI restriction endonuclease allowing discrimination among the four root-knot species. mtDNA was found to be a useful tool for discriminating between the important Meloidogyne species in this study.

Identification of Root-Knot Nematodes in the Mediterranean Region of Turkey by Using rDNA and mtDNA Markers

Fragments containing the internal transcribed spacer (ITS) ribosomal rDNA were amplified from Meloidogyne incognita, M. javanica, M. arenaria and M. hapla by polymerase chain reaction (PCR). Only digestion of the ITS region with the enzyme RsaI significantly distinguished M. hapla from other species, while other enzymes, EcoRI and BamHI, were not effective in distinguishing these nematode species. PCR was also done with mitocondrial DNA (mtDNA) markers for species discrimination. The obtained amplification product of 600 bp was digested with HinfI restriction endonuclease allowing discrimination among the four root-knot species. mtDNA was found to be a useful tool for discriminating between the important Meloidogyne species in this study.

Kaynak Göster

Bibtex @ { tbtkagriculture138638, journal = {Turkish Journal of Agriculture and Forestry}, issn = {1300-011X}, eissn = {1303-6173}, address = {}, publisher = {TÜBİTAK}, year = {2002}, volume = {26}, pages = {337 - 341}, doi = {}, title = {Identification of Root-Knot Nematodes in the Mediterranean Region of Turkey by Using rDNA and mtDNA Markers}, key = {cite}, author = {Devran, Zübeyir} }
APA Devran, Z . (2002). Identification of Root-Knot Nematodes in the Mediterranean Region of Turkey by Using rDNA and mtDNA Markers . Turkish Journal of Agriculture and Forestry , 26 (6) , 337-341 .
MLA Devran, Z . "Identification of Root-Knot Nematodes in the Mediterranean Region of Turkey by Using rDNA and mtDNA Markers" . Turkish Journal of Agriculture and Forestry 26 (2002 ): 337-341 <
Chicago Devran, Z . "Identification of Root-Knot Nematodes in the Mediterranean Region of Turkey by Using rDNA and mtDNA Markers". Turkish Journal of Agriculture and Forestry 26 (2002 ): 337-341
RIS TY - JOUR T1 - Identification of Root-Knot Nematodes in the Mediterranean Region of Turkey by Using rDNA and mtDNA Markers AU - Zübeyir Devran Y1 - 2002 PY - 2002 N1 - DO - T2 - Turkish Journal of Agriculture and Forestry JF - Journal JO - JOR SP - 337 EP - 341 VL - 26 IS - 6 SN - 1300-011X-1303-6173 M3 - UR - Y2 - 2021 ER -
EndNote %0 Turkish Journal of Agriculture and Forestry Identification of Root-Knot Nematodes in the Mediterranean Region of Turkey by Using rDNA and mtDNA Markers %A Zübeyir Devran %T Identification of Root-Knot Nematodes in the Mediterranean Region of Turkey by Using rDNA and mtDNA Markers %D 2002 %J Turkish Journal of Agriculture and Forestry %P 1300-011X-1303-6173 %V 26 %N 6 %R %U
ISNAD Devran, Zübeyir . "Identification of Root-Knot Nematodes in the Mediterranean Region of Turkey by Using rDNA and mtDNA Markers". Turkish Journal of Agriculture and Forestry 26 / 6 (Haziran 2002): 337-341 .
AMA Devran Z . Identification of Root-Knot Nematodes in the Mediterranean Region of Turkey by Using rDNA and mtDNA Markers. Turkish Journal of Agriculture and Forestry. 2002; 26(6): 337-341.
Vancouver Devran Z . Identification of Root-Knot Nematodes in the Mediterranean Region of Turkey by Using rDNA and mtDNA Markers. Turkish Journal of Agriculture and Forestry. 2002; 26(6): 337-341.
IEEE Z. Devran , "Identification of Root-Knot Nematodes in the Mediterranean Region of Turkey by Using rDNA and mtDNA Markers", Turkish Journal of Agriculture and Forestry, c. 26, sayı. 6, ss. 337-341, Haz. 2002