ELZBIETA KACZMARSKA, JACEK GAWRONSKI, MAGDALENA DYDUCH-SIEMINSKA, AGNIESZKA NAJDA, WOJCIECH MARECKI, JADWIGA ZEBROWSKA

Genetic diversity and chemical characterization of selected Polish and Russian cultivars and clones of blue honeysuckle (Lonicera caerulea)

In traditional medicine, Lonicera caerulea has a notable place, and it has been used for thousands of years in East Asian countries for treating cancer, inflammation, hepatic complications, influenza, and wounds. However, the molecular and chemical characterization of this plant is not well defined. In this study, inter-simple sequence repeat (ISSR) markers were used to determine the genetic variability of 7 blue honeysuckle cultivars and 3 clones. To characterize these 10 accessions, total flavonoids, phenolic acids contents, and antioxidant activities were estimated. Twelve primers generated 101 polymorphic ISSR-polymerase chain reaction bands. Based on the ISSR amplification profiles, a cluster dendrogram was obtained, which showed that the similarity coefficients among the 10 genotypes of L. Caerulea ranged from 0.53 to 0.69. The study also identified promising genotypes in terms of flavonoid and phenolic acid contents and high antioxidant activities, as follows: cultivar Atut and clone P. A systematic cluster analysis conducted on the basis of chemical data showed that the 10 tested genotypes can be classified into 2 groups. The ISSR analysis together with data for antioxidant activities as well as flavonoid and phenolic acid contents in honeysuckle could be used to evaluate the genetic diversity and to make more efficient choices of parents in current blue honeysuckle breeding programs.

Anahtar Kelimeler:

DPPH (diphenyl picrylhydrazyl), flavonoids, genetic diversity, ISSR markers, Lonicera caerulea, phenolic acids

Genetic diversity and chemical characterization of selected Polish and Russian cultivars and clones of blue honeysuckle (Lonicera caerulea)

Keywords:

DPPH (diphenyl picrylhydrazyl), flavonoids, genetic diversity, ISSR markers, Lonicera caerulea, phenolic acids,

PDF

___

Chaovanalikit A, Thompson M, Wrolstad R (2004). Characterization and quantification of anthocyanins and polyphenolics in blue honeysuckle (Lonicera caerulea L.). J Agr Food Chem 52: 848– 852.
Chen D, Zhang X, Li L (2012). Analysis of genetic diversity and genetic relationships of Lonicera macranthoides cultivars. Zhongguo Zhong Yao Za Zhi 37: 1912–1916 (in Chinese with English abstract).
Chen JH, Ho CT (1997). Antioxidant activities of caffeic acid and its related hydroxycinnamic acid compounds. J Agr Food Chem 45: 2374–2378.
Debnath SC (2007). Inter simple sequence repeat (ISSR) to assess genetic diversity within a collection of wild lingonberry (Vaccinium vitisidaea L.) clones. Can J Plant Sci 87: 337–344.
Debnath SC, Ricard E (2009). ISSR, anthocyanin content and antioxidant activity analyses to characterize strawberry genotypes. J Appl Hort 11: 83–89.
Doyle JJ, Doyle JL (1987). A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem Bull 19: 11–15.
Frejnagel S (2007). Comparison of polyphenolic composition of extracts from honeysuckle, chokeberries and green tea – a short report. Pol J Food Nutr Sci 57: 83–86.
Garcia MG, Ontivero M, Diaz Ricci JC, Castagnaro A (2002). Morphological traits and high resolution RAPD markers for the identification of the main strawberry varieties cultivated in Argentina. Plant Breeding 121: 76–80.
Gilbert JE, Lewis RV, Wilkinson MJ, Caligari PDS (1999). Developing an appropriate strategy to assess genetic variability in plant germplasm collections. Theor Appl Genet 98: 1125–1131.
Hoppula KB, Karhu ST (2006). Strawberry fruit quality responses to the production environment. J Food Agri Environ 4: 166–170. Hummer KE (2006). Blue honeysuckle: a new berry crop for North America. J Am Pomol Soc 60: 3–8.
Jurikova T, Sochor J, Rop O, Mlček J, Balla Š, Szekeres L, Žitný R, Zitka O, Adam V, Kizek R (2012). Evaluation of polyphenolic profile and nutritional value of non-traditional fruit species in the Czech Republic - a comparative study. Molecules 17: 8968–8981.
Lamoureux D, Sorokin A, Lefevre I, Alexanian S, Eyzaguirre P, Hausman JF (2011). Investigation of genetic diversity in Russian collections of raspberry and blue honeysuckle. Plant Genet Resources 9: 202–205.
Langridge P, Chalmers K (2004). The principle: identification and application of molecular markers. In: Lörz H, Wenzel G, editors. Biotechnology in Agriculture and Forestry. New York, NY, USA: Spring, pp. 3–22.
Małodobry M, Bieniasz M, Dziedzic E (2010). Evaluation of the yield and some components in the fruit of blue honeysuckle (Lonicera caerulea var. edulis Turcz. Freyn.). Folia Horticulturae 22/1: 45–50.
Miyashita T, Ohashi T, Shibata F, Araki H, Hoshino Y (2009). Plant regeneration with maintenance of the endosperm ploidy level by endosperm culture in Lonicera caerulea var. emphyllocalyx. Plant Cell Tiss Org 98: 291–301.
Morales RGF, Resende JTV, Faria MV, Andrade MC, Resende LV, Delatorre CA, Silva PR (2011). Genetic similarity among strawberry cultivars assessed by RAPD and ISSR markers. Sci Agr 68: 665–670.
Najda A, Dyduch-Siemińska M, Dyduch J, Gantner M (2014). Comparative analysis of secondary metabolites content in Fragaria vesca L fruits. Ann Agr Env Med 21: 243–252.
Naugžemys D, Žilinskaitė S, Kleizaitė V, Skridaila A, Žvingila D (2011). Assessment of genetic variation among elite and wild germplasm of blue honeysuckle (Lonicera caerulea L). Balt For 17: 8–16.
Nei M, Li WH (1979). Mathematical model for studying genetic variation in terms of restriction endonucleases. P Natl Acad Sci USA 76: 5269–5273.
Ochmian I, Skupień K, Grajkowski J, Smolik M, Ostrowska K (2012). Chemical composition and physical characteristics of fruits of two cultivars of blue honeysuckle (Lonicera caerulea L.) in relation to their degree of maturity and harvest date. Not Bot Horti Agrobo 40: 155–162.
Polish Pharmaceutical Society (2005). Polish Pharmacopoeia VII. Warsaw, Poland: Polish Pharmaceutical Society
Polish Pharmaceutical Society (2011). Polish Pharmacopoeia IX. Warsaw, Poland: Polish Pharmaceutical Society.
Prevost A, Wilkinson MJ (1999). A new system of comparing PCR primers applied to ISSR fingerprinting of potato cultivars. Theor Appl Genet 98: 107–112.
Rohlf FJ (2001). NTSYS-pc Numerical Taxonomy and Multivariate Analysis System. Version 5.1. Setauket, NY, USA: Exeter Publishing Ltd.
Sestili S, Giardini A, Ficcadenti N (2011). Genetic diversity among Italian melon inodorus (Cucumis melo L.) germplasm revealed by ISSR analysis and agronomic trait. Plant Genet Resources 9: 214–217.
Skupień K, Ochmian I, Grajkowski J (2009). Influence of ripening time on fruit chemical composition of two blue honeysuckle cultigens. J Fruit Ornam Plant Res 17: 101–111.
Skupień K, Oszmiański J, Ochmian I, Grajkowski J (2007). Characterization of selected physico-chemical features of blue honeysuckle fruit cultivar Zielona. Polish Journal of Natural Sciences 4: 101–107.
Smolik M, Ochmian I, Grajkowski J (2010). Genetic variability of Polish and Russian accessions of cultivated blue honeysuckle (Lonicera caerulea). Plant Genetics 46: 960–966.
Smolik M, Zieliński J, Rzepka-Plevneš D, Adamska K (2006). Polymorphism of microsatellite sequences in morphologically and phenologically different genotypes of Lonicera periclymenu. J Food Agri Environ 4: 226–233.
Strelcina SA, Sorokin AA, Plekhanova MN, Lobanova EV (2006). Sostav biologičeski aktivnyh fenolnyh soiedinienii sortov zhimolosti v usloviah severno-zapadnoi zony plodovodstva RF. Agrarnaia Rossia 6: 67–72 (in Russian).
Sun F, Huo J, Qin D (2011). Establishment and optimization of SRAP amplification system in Lonicara caerulea L. Journal of Northeast Agricultural University (English Edition) 18: 26–31.
Svarcova I, Heinrich J, Valentova K (2007). Berry fruits as a source of biologically active compounds: the case of Lonicera caerulea. Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub 151: 163–174.
Wang X, Xie B, Li J, Zeng H, Li Y (2008). Analysis of honeysuckles’ genetic diversity by ISSR molecular markers. Journal of Central South University of Forestry & Technology 2008: 141–147.
Zadernowski R, Naczk M, Nestorowicz J (2005). Phenolic acid profiles in some small berries. J Agr Food Chem 53: 2118–2124.