Luis GALIANA BALAGUER, Jaime CEBOLLA CORNEJO, Gines IBANEZ, Salvador ROSELLO

Evaluation of germplasm in Solanum section Lycopersicon for tomato taste improvement

The genetic potential of accessions from Solanum section Lycopersicon (S. lycopersicum L., S. lycopersicum var. cerasiforme, Spimpinellifolium L., and S. habrochaites Knaap & Spooner) for breeding tomato taste has been studied in three environments with clonalreplicates. The environment clearly affected the accumulation and level of variation of sugars and acids and derived variables througha direct effect. It seems that photosynthetically active radiation would exert a major effect on sugar accumulation while in the caseof organic acids the effect of temperature might be more important. Even more, important genotype × environment interactions canconsiderably modify the real value of germplasm, being considerably higher in wild species. The environment affected not only meancontents but also the levels of variation. Thus, the need to develop multienvironmental screening programs is suggested to identifysolid sources of variation. An important intraaccession variability was also found in wild germplasm, emphasizing the need to analyzea high number of plants per accession in order to identify sources of variation. Accessions with a significant genotypic contribution tothe accumulation of sucrose within the Lycopersicon group were identified and may be interesting to analyze the regulation of vacuolarinvertase. Accessions with different genotypic contributions to citric, malic, and glutamic acid accumulation have also been identified.These accessions will be valuable for the development of breeding programs considering the acid component of taste. Additionally, thesegenetic resources will be interesting to study the regulation of the tricarboxylic acid cycle and the gamma-aminobutyric acid shunt.

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